GCKR Mouse Monoclonal Antibody [Clone ID: OTI1E7]

CAT#: TA502152

GCKR mouse monoclonal antibody, clone OTI1E7 (formerly 1E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GCKR (Cat# RC214230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCKR antibody (Cat# TA502152). Positive lysates LY400576 (100ug) and LC400576 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GCKR monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GCKR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502152)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GCKR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502152)

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  Anti-GCKR mouse monoclonal antibody (TA502152) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GCKR (RC214230).

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Specifications

Product Data

• Clone Name: OTI1E7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GCKR (NP_001417) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.5 kDa
• Gene Name: glucokinase regulator
• Database Link:
  NP_001477
  Entrez Gene 25658 RatEntrez Gene 231103 MouseEntrez Gene 2646 Human
  UniProt ID Q14397
• Background: This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq]
• Synonyms: FGQTL5; GKRP