NCOA2 (NM_006540) Human Tagged ORF Clone

CAT#: RG212235

NCOA2 (tGFP-tagged) - Human nuclear receptor coactivator 2 (NCOA2)

Product Images

Circular map for RG212235

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NCOA2
• Synonyms: bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212235 representing NM_006540
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGGATGGGAGAAAATACCTCTGACCCCTCCAGGGCAGAGACAAGAAAGCGCAAGGAATGTCCTG
  ACCAACTTGGACCCAGCCCCAAAAGGAACACTGAAAAACGTAATCGTGAACAGGAAAATAAATATATAGA
  AGAACTTGCAGAGTTGATTTTTGCAAATTTTAATGATATAGACAACTTTAACTTCAAACCTGACAAATGT
  GCAATCTTAAAAGAAACTGTGAAGCAAATTCGTCAGATCAAAGAACAAGAGAAAGCAGCAGCTGCCAACA
  TAGATGAAGTGCAGAAGTCAGATGTATCCTCTACAGGGCAGGGTGTCATCGACAAGGATGCGCTGGGGCC
  TATGATGCTTGAGGCCCTTGATGGGTTCTTCTTTGTAGTGAACCTGGAAGGCAACGTTGTGTTTGTGTCA
  GAGAATGTGACACAGTATCTAAGGTATAACCAAGAAGAGCTGATGAACAAAAGTGTATATAGCATCTTGC
  ATGTTGGGGACCACACGGAATTTGTCAAAAACCTGCTGCCAAAGTCTATAGTAAATGGGGGATCTTGGTC
  TGGCGAACCTCCGAGGCGGAACAGCCATACCTTCAATTGTCGGATGCTGGTAAAACCTTTACCTGATTCA
  GAAGAGGAGGGTCATGATAACCAGGAAGCTCATCAGAAATATGAAACTATGCAGTGCTTCGCTGTCTCTC
  AACCAAAGTCCATCAAAGAAGAAGGAGAAGATTTGCAGTCCTGCTTGATTTGCGTGGCAAGAAGAGTTCC
  CATGAAGGAAAGACCAGTTCTTCCCTCATCAGAAAGTTTTACTACTCGCCAGGATCTCCAAGGCAAGATC
  ACGTCTCTGGATACCAGCACCATGAGAGCAGCCATGAAACCAGGCTGGGAGGACCTGGTAAGAAGGTGTA
  TTCAGAAGTTCCATGCGCAGCATGAAGGAGAATCTGTGTCCTATGCTAAGAGGCATCATCATGAAGTACT
  GAGACAAGGATTGGCATTCAGTCAAATCTATCGTTTTTCCTTGTCTGATGGCACTCTTGTTGCTGCACAA
  ACGAAGAGCAAACTCATCCGTTCTCAGACTACTAATGAACCTCAACTTGTAATATCTTTACATATGCTTC
  ACAGAGAGCAGAATGTGTGTGTGATGAATCCGGATCTGACTGGACAAACGATGGGGAAGCCACTGAATCC
  AATTAGCTCTAACAGCCCTGCCCATCAGGCCCTGTGCAGTGGGAACCCAGGTCAGGACATGACCCTCAGT
  AGCAATATAAATTTTCCCATAAATGGCCCAAAGGAACAAATGGGCATGCCCATGGGCAGGTTTGGTGGTT
  CTGGGGGAATGAACCATGTGTCAGGCATGCAAGCAACCACTCCTCAGGGTAGTAACTATGCACTCAAAAT
  GAACAGCCCCTCACAAAGCAGCCCTGGCATGAATCCAGGACAGCCCACCTCCATGCTTTCACCAAGGCAT
  CGCATGAGCCCTGGAGTGGCTGGCAGCCCTCGAATCCCACCCAGTCAGTTTTCCCCTGCAGGAAGCTTGC
  ATTCCCCTGTGGGAGTTTGCAGCAGCACAGGAAATAGCCATAGTTATACCAACAGCTCCCTCAATGCACT
  TCAGGCCCTCAGCGAGGGGCACGGGGTCTCATTAGGGTCATCGTTGGCTTCACCAGACCTAAAAATGGGC
  AATTTGCAAAACTCCCCAGTTAATATGAATCCTCCCCCACTCAGCAAGATGGGAAGCTTGGACTCAAAAG
  ACTGTTTTGGACTATATGGGGAGCCCTCTGAAGGTACAACTGGACAAGCAGAGAGCAGCTGCCATCCTGG
  AGAGCAAAAGGAAACAAATGACCCCAACCTGCCCCCGGCCGTGAGCAGTGAGAGAGCTGACGGGCAGAGC
  AGACTGCATGACAGCAAAGGGCAGACCAAACTCCTGCAGCTGCTGACCACCAAATCTGATCAGATGGAGC
  CCTCGCCCTTAGCCAGCTCTTTGTCGGATACAAACAAAGACTCCACAGGTAGCTTGCCTGGTTCTGGGTC
  TACACATGGAACCTCGCTCAAGGAGAAGCATAAAATTTTGCACAGACTCTTGCAGGACAGCAGTTCCCCT
  GTGGACTTGGCCAAGTTAACAGCAGAAGCCACAGGCAAAGACCTGAGCCAGGAGTCCAGCAGCACAGCTC
  CTGGATCAGAAGTGACTATTAAACAAGAGCCGGTGAGCCCCAAGAAGAAAGAGAATGCACTACTTCGCTA
  TTTGCTAGATAAAGATGATACTAAAGATATTGGTTTACCAGAAATAACCCCCAAACTTGAGAGACTGGAC
  AGTAAGACAGATCCTGCCAGTAACACAAAATTAATAGCAATGAAAACTGAGAAGGAGGAGATGAGCTTTG
  AGCCTGGTGACCAGCCTGGCAGTGAGCTGGACAACTTGGAGGAGATTTTGGATGATTTGCAGAATAGTCA
  ATTACCACAGCTTTTCCCAGACACGAGGCCAGGCGCCCCTGCTGGATCAGTTGACAAGCAAGCCATCATC
  AATGACCTCATGCAACTCACAGCTGAAAACAGCCCTGTCACACCTGTTGGAGCCCAGAAAACAGCACTGC
  GAATTTCACAGAGCACTTTTAATAACCCACGACCAGGGCAACTGGGCAGGTTATTGCCAAACCAGAATTT
  ACCACTTGACATCACATTGCAAAGCCCAACTGGTGCTGGACCTTTCCCACCAATCAGAAACAGTAGTCCC
  TACTCAGTGATACCTCAGCCAGGAATGATGGGTAATCAAGGGATGATAGGAAACCAAGGAAATTTAGGGA
  ACAGTAGCACAGGAATGATTGGTAACAGTGCTTCTCGGCCTACTATGCCATCTGGAGAATGGGCACCGCA
  GAGTTCGGCTGTGAGAGTCACCTGTGCTGCTACCACCAGTGCCATGAACCGGCCAGTCCAAGGAGGTATG
  ATTCGGAACCCAGCAGCCAGCATCCCCATGAGGCCCAGCAGCCAGCCTGGCCAAAGACAGACGCTTCAGT
  CTCAGGTCATGAATATAGGGCCATCTGAATTAGAGATGAACATGGGGGGACCTCAGTATAGCCAACAACA
  AGCTCCTCCAAATCAGACTGCCCCATGGCCTGAAAGCATCCTGCCTATAGACCAGGCGTCTTTTGCCAGC
  CAAAACAGGCAGCCATTTGGCAGTTCTCCAGATGACTTGCTATGTCCACATCCTGCAGCTGAGTCTCCGA
  GTGATGAGGGAGCTCTCCTGGACCAGCTGTATCTGGCCTTGCGGAATTTTGATGGCCTGGAGGAGATTGA
  TAGAGCCTTAGGAATACCCGAACTGGTCAGCCAGAGCCAAGCAGTAGATCCAGAACAGTTCTCAAGTCAG
  GATTCCAACATCATGCTGGAGCAGAAGGCGCCCGTTTTCCCACAGCAGTATGCATCTCAGGCACAAATGG
  CCCAGGGTAGCTATTCTCCCATGCAAGATCCAAACTTTCACACCATGGGACAGCGGCCTAGTTATGCCAC
  ACTCCGTATGCAGCCCAGACCGGGCCTCAGGCCCACGGGCCTAGTGCAGAACCAGCCAAATCAACTAAGA
  CTTCAACTTCAGCATCGCCTCCAAGCACAGCAGAATCGCCAGCCACTTATGAATCAAATCAGCAATGTTT
  CCAATGTGAACTTGACTCTGAGGCCTGGAGTACCAACACAGGCACCTATTAATGCACAGATGCTGGCCCA
  GAGACAGAGGGAAATCCTGAACCAGCATCTTCGACAGAGACAAATGCATCAGCAACAGCAAGTTCAGCAA
  CGAACTTTGATGATGAGAGGACAAGGGTTGAATATGACACCAAGCATGGTGGCTCCTAGTGGTATCCCAG
  CAACTATGAGCAACCCTCGGATTCCCCAGGCAAATGCACAGCAGTTTCCATTTCCTCCAAACTACGGAAT
  AAGTCAGCAACCTGATCCAGGCTTTACTGGGGCTACGACTCCCCAGAGCCCACTTATGTCACCCCGAATG
  GCACATACACAGAGTCCCATGATGCAACAGTCTCAGGCCAACCCAGCCTATCAGGCCCCCTCCGACATAA
  ATGGATGGGCGCAGGGGAACATGGGCGGAAACAGCATGTTTTCCCAGCAGTCCCCACCACACTTTGGGCA
  GCAAGCAAACACCAGCATGTACAGTAACAACATGAACATCAATGTGTCCATGGCGACCAACACAGGTGGC
  ATGAGCAGCATGAACCAGATGACAGGACAGATCAGCATGACCTCAGTGACCTCCGTGCCTACGTCAGGGC
  TGTCCTCCATGGGTCCCGAGCAGGTTAATGATCCTGCTCTGAGGGGAGGCAACCTGTTCCCAAACCAGCT
  GCCTGGAATGGATATGATTAAGCAGGAGGGAGACACAACACGGAAATATTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212235 representing NM_006540
  Red=Cloning site Green=Tags(s)
  
  MSGMGENTSDPSRAETRKRKECPDQLGPSPKRNTEKRNREQENKYIEELAELIFANFNDIDNFNFKPDKC
  AILKETVKQIRQIKEQEKAAAANIDEVQKSDVSSTGQGVIDKDALGPMMLEALDGFFFVVNLEGNVVFVS
  ENVTQYLRYNQEELMNKSVYSILHVGDHTEFVKNLLPKSIVNGGSWSGEPPRRNSHTFNCRMLVKPLPDS
  EEEGHDNQEAHQKYETMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPVLPSSESFTTRQDLQGKI
  TSLDTSTMRAAMKPGWEDLVRRCIQKFHAQHEGESVSYAKRHHHEVLRQGLAFSQIYRFSLSDGTLVAAQ
  TKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQTMGKPLNPISSNSPAHQALCSGNPGQDMTLS
  SNINFPINGPKEQMGMPMGRFGGSGGMNHVSGMQATTPQGSNYALKMNSPSQSSPGMNPGQPTSMLSPRH
  RMSPGVAGSPRIPPSQFSPAGSLHSPVGVCSSTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDLKMG
  NLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAESSCHPGEQKETNDPNLPPAVSSERADGQS
  RLHDSKGQTKLLQLLTTKSDQMEPSPLASSLSDTNKDSTGSLPGSGSTHGTSLKEKHKILHRLLQDSSSP
  VDLAKLTAEATGKDLSQESSSTAPGSEVTIKQEPVSPKKKENALLRYLLDKDDTKDIGLPEITPKLERLD
  SKTDPASNTKLIAMKTEKEEMSFEPGDQPGSELDNLEEILDDLQNSQLPQLFPDTRPGAPAGSVDKQAII
  NDLMQLTAENSPVTPVGAQKTALRISQSTFNNPRPGQLGRLLPNQNLPLDITLQSPTGAGPFPPIRNSSP
  YSVIPQPGMMGNQGMIGNQGNLGNSSTGMIGNSASRPTMPSGEWAPQSSAVRVTCAATTSAMNRPVQGGM
  IRNPAASIPMRPSSQPGQRQTLQSQVMNIGPSELEMNMGGPQYSQQQAPPNQTAPWPESILPIDQASFAS
  QNRQPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALRNFDGLEEIDRALGIPELVSQSQAVDPEQFSSQ
  DSNIMLEQKAPVFPQQYASQAQMAQGSYSPMQDPNFHTMGQRPSYATLRMQPRPGLRPTGLVQNQPNQLR
  LQLQHRLQAQQNRQPLMNQISNVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMHQQQQVQQ
  RTLMMRGQGLNMTPSMVAPSGIPATMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATTPQSPLMSPRM
  AHTQSPMMQQSQANPAYQAPSDINGWAQGNMGGNSMFSQQSPPHFGQQANTSMYSNNMNINVSMATNTGG
  MSSMNQMTGQISMTSVTSVPTSGLSSMGPEQVNDPALRGGNLFPNQLPGMDMIKQEGDTTRKYC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006540
• ORF Size: 4392 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006540.4
• RefSeq Size: 6157 bp
• RefSeq ORF: 4395 bp
• Locus ID: 10499
• UniProt ID: Q15596
• Cytogenetics: 8q13.3
• Domains: PAS, HLH
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]