NCOA2 (NM_006540) Human Tagged ORF Clone

CAT#: RC212235

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NCOA2 (Myc-DDK-tagged)-Human nuclear receptor coactivator 2 (NCOA2)



  "NM_006540" in other vectors (6)

Reconstitution Protocol

USD 1,878.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "NCOA2"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NCOA2
Synonyms bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212235 representing NM_006540
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGGGATGGGAGAAAATACCTCTGACCCCTCCAGGGCAGAGACAAGAAAGCGCAAGGAATGTCCTG
ACCAACTTGGACCCAGCCCCAAAAGGAACACTGAAAAACGTAATCGTGAACAGGAAAATAAATATATAGA
AGAACTTGCAGAGTTGATTTTTGCAAATTTTAATGATATAGACAACTTTAACTTCAAACCTGACAAATGT
GCAATCTTAAAAGAAACTGTGAAGCAAATTCGTCAGATCAAAGAACAAGAGAAAGCAGCAGCTGCCAACA
TAGATGAAGTGCAGAAGTCAGATGTATCCTCTACAGGGCAGGGTGTCATCGACAAGGATGCGCTGGGGCC
TATGATGCTTGAGGCCCTTGATGGGTTCTTCTTTGTAGTGAACCTGGAAGGCAACGTTGTGTTTGTGTCA
GAGAATGTGACACAGTATCTAAGGTATAACCAAGAAGAGCTGATGAACAAAAGTGTATATAGCATCTTGC
ATGTTGGGGACCACACGGAATTTGTCAAAAACCTGCTGCCAAAGTCTATAGTAAATGGGGGATCTTGGTC
TGGCGAACCTCCGAGGCGGAACAGCCATACCTTCAATTGTCGGATGCTGGTAAAACCTTTACCTGATTCA
GAAGAGGAGGGTCATGATAACCAGGAAGCTCATCAGAAATATGAAACTATGCAGTGCTTCGCTGTCTCTC
AACCAAAGTCCATCAAAGAAGAAGGAGAAGATTTGCAGTCCTGCTTGATTTGCGTGGCAAGAAGAGTTCC
CATGAAGGAAAGACCAGTTCTTCCCTCATCAGAAAGTTTTACTACTCGCCAGGATCTCCAAGGCAAGATC
ACGTCTCTGGATACCAGCACCATGAGAGCAGCCATGAAACCAGGCTGGGAGGACCTGGTAAGAAGGTGTA
TTCAGAAGTTCCATGCGCAGCATGAAGGAGAATCTGTGTCCTATGCTAAGAGGCATCATCATGAAGTACT
GAGACAAGGATTGGCATTCAGTCAAATCTATCGTTTTTCCTTGTCTGATGGCACTCTTGTTGCTGCACAA
ACGAAGAGCAAACTCATCCGTTCTCAGACTACTAATGAACCTCAACTTGTAATATCTTTACATATGCTTC
ACAGAGAGCAGAATGTGTGTGTGATGAATCCGGATCTGACTGGACAAACGATGGGGAAGCCACTGAATCC
AATTAGCTCTAACAGCCCTGCCCATCAGGCCCTGTGCAGTGGGAACCCAGGTCAGGACATGACCCTCAGT
AGCAATATAAATTTTCCCATAAATGGCCCAAAGGAACAAATGGGCATGCCCATGGGCAGGTTTGGTGGTT
CTGGGGGAATGAACCATGTGTCAGGCATGCAAGCAACCACTCCTCAGGGTAGTAACTATGCACTCAAAAT
GAACAGCCCCTCACAAAGCAGCCCTGGCATGAATCCAGGACAGCCCACCTCCATGCTTTCACCAAGGCAT
CGCATGAGCCCTGGAGTGGCTGGCAGCCCTCGAATCCCACCCAGTCAGTTTTCCCCTGCAGGAAGCTTGC
ATTCCCCTGTGGGAGTTTGCAGCAGCACAGGAAATAGCCATAGTTATACCAACAGCTCCCTCAATGCACT
TCAGGCCCTCAGCGAGGGGCACGGGGTCTCATTAGGGTCATCGTTGGCTTCACCAGACCTAAAAATGGGC
AATTTGCAAAACTCCCCAGTTAATATGAATCCTCCCCCACTCAGCAAGATGGGAAGCTTGGACTCAAAAG
ACTGTTTTGGACTATATGGGGAGCCCTCTGAAGGTACAACTGGACAAGCAGAGAGCAGCTGCCATCCTGG
AGAGCAAAAGGAAACAAATGACCCCAACCTGCCCCCGGCCGTGAGCAGTGAGAGAGCTGACGGGCAGAGC
AGACTGCATGACAGCAAAGGGCAGACCAAACTCCTGCAGCTGCTGACCACCAAATCTGATCAGATGGAGC
CCTCGCCCTTAGCCAGCTCTTTGTCGGATACAAACAAAGACTCCACAGGTAGCTTGCCTGGTTCTGGGTC
TACACATGGAACCTCGCTCAAGGAGAAGCATAAAATTTTGCACAGACTCTTGCAGGACAGCAGTTCCCCT
GTGGACTTGGCCAAGTTAACAGCAGAAGCCACAGGCAAAGACCTGAGCCAGGAGTCCAGCAGCACAGCTC
CTGGATCAGAAGTGACTATTAAACAAGAGCCGGTGAGCCCCAAGAAGAAAGAGAATGCACTACTTCGCTA
TTTGCTAGATAAAGATGATACTAAAGATATTGGTTTACCAGAAATAACCCCCAAACTTGAGAGACTGGAC
AGTAAGACAGATCCTGCCAGTAACACAAAATTAATAGCAATGAAAACTGAGAAGGAGGAGATGAGCTTTG
AGCCTGGTGACCAGCCTGGCAGTGAGCTGGACAACTTGGAGGAGATTTTGGATGATTTGCAGAATAGTCA
ATTACCACAGCTTTTCCCAGACACGAGGCCAGGCGCCCCTGCTGGATCAGTTGACAAGCAAGCCATCATC
AATGACCTCATGCAACTCACAGCTGAAAACAGCCCTGTCACACCTGTTGGAGCCCAGAAAACAGCACTGC
GAATTTCACAGAGCACTTTTAATAACCCACGACCAGGGCAACTGGGCAGGTTATTGCCAAACCAGAATTT
ACCACTTGACATCACATTGCAAAGCCCAACTGGTGCTGGACCTTTCCCACCAATCAGAAACAGTAGTCCC
TACTCAGTGATACCTCAGCCAGGAATGATGGGTAATCAAGGGATGATAGGAAACCAAGGAAATTTAGGGA
ACAGTAGCACAGGAATGATTGGTAACAGTGCTTCTCGGCCTACTATGCCATCTGGAGAATGGGCACCGCA
GAGTTCGGCTGTGAGAGTCACCTGTGCTGCTACCACCAGTGCCATGAACCGGCCAGTCCAAGGAGGTATG
ATTCGGAACCCAGCAGCCAGCATCCCCATGAGGCCCAGCAGCCAGCCTGGCCAAAGACAGACGCTTCAGT
CTCAGGTCATGAATATAGGGCCATCTGAATTAGAGATGAACATGGGGGGACCTCAGTATAGCCAACAACA
AGCTCCTCCAAATCAGACTGCCCCATGGCCTGAAAGCATCCTGCCTATAGACCAGGCGTCTTTTGCCAGC
CAAAACAGGCAGCCATTTGGCAGTTCTCCAGATGACTTGCTATGTCCACATCCTGCAGCTGAGTCTCCGA
GTGATGAGGGAGCTCTCCTGGACCAGCTGTATCTGGCCTTGCGGAATTTTGATGGCCTGGAGGAGATTGA
TAGAGCCTTAGGAATACCCGAACTGGTCAGCCAGAGCCAAGCAGTAGATCCAGAACAGTTCTCAAGTCAG
GATTCCAACATCATGCTGGAGCAGAAGGCGCCCGTTTTCCCACAGCAGTATGCATCTCAGGCACAAATGG
CCCAGGGTAGCTATTCTCCCATGCAAGATCCAAACTTTCACACCATGGGACAGCGGCCTAGTTATGCCAC
ACTCCGTATGCAGCCCAGACCGGGCCTCAGGCCCACGGGCCTAGTGCAGAACCAGCCAAATCAACTAAGA
CTTCAACTTCAGCATCGCCTCCAAGCACAGCAGAATCGCCAGCCACTTATGAATCAAATCAGCAATGTTT
CCAATGTGAACTTGACTCTGAGGCCTGGAGTACCAACACAGGCACCTATTAATGCACAGATGCTGGCCCA
GAGACAGAGGGAAATCCTGAACCAGCATCTTCGACAGAGACAAATGCATCAGCAACAGCAAGTTCAGCAA
CGAACTTTGATGATGAGAGGACAAGGGTTGAATATGACACCAAGCATGGTGGCTCCTAGTGGTATCCCAG
CAACTATGAGCAACCCTCGGATTCCCCAGGCAAATGCACAGCAGTTTCCATTTCCTCCAAACTACGGAAT
AAGTCAGCAACCTGATCCAGGCTTTACTGGGGCTACGACTCCCCAGAGCCCACTTATGTCACCCCGAATG
GCACATACACAGAGTCCCATGATGCAACAGTCTCAGGCCAACCCAGCCTATCAGGCCCCCTCCGACATAA
ATGGATGGGCGCAGGGGAACATGGGCGGAAACAGCATGTTTTCCCAGCAGTCCCCACCACACTTTGGGCA
GCAAGCAAACACCAGCATGTACAGTAACAACATGAACATCAATGTGTCCATGGCGACCAACACAGGTGGC
ATGAGCAGCATGAACCAGATGACAGGACAGATCAGCATGACCTCAGTGACCTCCGTGCCTACGTCAGGGC
TGTCCTCCATGGGTCCCGAGCAGGTTAATGATCCTGCTCTGAGGGGAGGCAACCTGTTCCCAAACCAGCT
GCCTGGAATGGATATGATTAAGCAGGAGGGAGACACAACACGGAAATATTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212235 representing NM_006540
Red=Cloning site Green=Tags(s)

MSGMGENTSDPSRAETRKRKECPDQLGPSPKRNTEKRNREQENKYIEELAELIFANFNDIDNFNFKPDKC
AILKETVKQIRQIKEQEKAAAANIDEVQKSDVSSTGQGVIDKDALGPMMLEALDGFFFVVNLEGNVVFVS
ENVTQYLRYNQEELMNKSVYSILHVGDHTEFVKNLLPKSIVNGGSWSGEPPRRNSHTFNCRMLVKPLPDS
EEEGHDNQEAHQKYETMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPVLPSSESFTTRQDLQGKI
TSLDTSTMRAAMKPGWEDLVRRCIQKFHAQHEGESVSYAKRHHHEVLRQGLAFSQIYRFSLSDGTLVAAQ
TKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQTMGKPLNPISSNSPAHQALCSGNPGQDMTLS
SNINFPINGPKEQMGMPMGRFGGSGGMNHVSGMQATTPQGSNYALKMNSPSQSSPGMNPGQPTSMLSPRH
RMSPGVAGSPRIPPSQFSPAGSLHSPVGVCSSTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDLKMG
NLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAESSCHPGEQKETNDPNLPPAVSSERADGQS
RLHDSKGQTKLLQLLTTKSDQMEPSPLASSLSDTNKDSTGSLPGSGSTHGTSLKEKHKILHRLLQDSSSP
VDLAKLTAEATGKDLSQESSSTAPGSEVTIKQEPVSPKKKENALLRYLLDKDDTKDIGLPEITPKLERLD
SKTDPASNTKLIAMKTEKEEMSFEPGDQPGSELDNLEEILDDLQNSQLPQLFPDTRPGAPAGSVDKQAII
NDLMQLTAENSPVTPVGAQKTALRISQSTFNNPRPGQLGRLLPNQNLPLDITLQSPTGAGPFPPIRNSSP
YSVIPQPGMMGNQGMIGNQGNLGNSSTGMIGNSASRPTMPSGEWAPQSSAVRVTCAATTSAMNRPVQGGM
IRNPAASIPMRPSSQPGQRQTLQSQVMNIGPSELEMNMGGPQYSQQQAPPNQTAPWPESILPIDQASFAS
QNRQPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALRNFDGLEEIDRALGIPELVSQSQAVDPEQFSSQ
DSNIMLEQKAPVFPQQYASQAQMAQGSYSPMQDPNFHTMGQRPSYATLRMQPRPGLRPTGLVQNQPNQLR
LQLQHRLQAQQNRQPLMNQISNVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMHQQQQVQQ
RTLMMRGQGLNMTPSMVAPSGIPATMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATTPQSPLMSPRM
AHTQSPMMQQSQANPAYQAPSDINGWAQGNMGGNSMFSQQSPPHFGQQANTSMYSNNMNINVSMATNTGG
MSSMNQMTGQISMTSVTSVPTSGLSSMGPEQVNDPALRGGNLFPNQLPGMDMIKQEGDTTRKYC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006540
ORF Size 4392 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006540.4
RefSeq Size 6157 bp
RefSeq ORF 4395 bp
Locus ID 10499
UniProt ID Q15596
Cytogenetics 8q13.3
Domains PAS, HLH
Protein Families Druggable Genome
MW 159 kDa
Gene Summary The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

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