NCOA2 (NM_006540) Human Untagged Clone

CAT#: SC316986

NCOA2 (untagged)-Human nuclear receptor coactivator 2 (NCOA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCOA2
• Synonyms: bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006540 edited
  CCGAGCCGATTTCTCTTGGATTTGGCTACACACTTATAGATCTTCTGCACTGTTTACAGG
  CACAGTTGCTGATATGTGTTCAAGATGAGTGGGATGGGAGAAAATACCTCTGACCCCTCC
  AGGGCAGAGACAAGAAAGCGCAAGGAATGTCCTGACCAACTTGGACCCAGCCCCAAAAGG
  AACACTGAAAAACGTAATCGTGAACAGGAAAATAAATATATAGAAGAACTTGCAGAGTTG
  ATTTTTGCAAATTTTAATGATATAGACAACTTTAACTTCAAACCTGACAAATGTGCAATC
  TTAAAAGAAACTGTGAAGCAAATTCGTCAGATCAAAGAACAAGAGAAAGCAGCAGCTGCC
  AACATAGATGAAGTGCAGAAGTCAGATGTATCCTCTACAGGGCAGGGTGTCATCGACAAG
  GATGCGCTGGGGCCTATGATGCTTGAGGCCCTTGATGGGTTCTTCTTTGTAGTGAACCTG
  GAAGGCAACGTTGTGTTTGTGTCAGAGAATGTGACACAGTATCTAAGGTATAACCAAGAA
  GAGCTGATGAACAAAAGTGTATATAGCATCTTGCATGTTGGGGACCACACGGAATTTGTC
  AAAAACCTGCTGCCAAAGTCTATAGTAAATGGGGGATCTTGGTCTGGCGAACCTCCGAGG
  CGGAACAGCCATACCTTCAATTGTCGGATGCTGGTAAAACCTTTACCTGATTCAGAAGAG
  GAGGGTCATGATAACCAGGAAGCTCATCAGAAATATGAAACTATGCAGTGCTTCGCTGTC
  TCTCAACCAAAGTCCATCAAAGAAGAAGGAGAAGATTTGCAGTCCTGCTTGATTTGCGTG
  GCAAGAAGAGTTCCCATGAAGGAAAGACCAGTTCTTCCCTCATCAGAAAGTTTTACTACT
  CGCCAGGATCTCCAAGGCAAGATCACGTCTCTGGATACCAGCACCATGAGAGCAGCCATG
  AAACCAGGCTGGGAGGACCTGGTAAGAAGGTGTATTCAGAAGTTCCATGCGCAGCATGAA
  GGAGAATCTGTGTCCTATGCTAAGAGGCATCATCATGAAGTACTGAGACAAGGATTGGCA
  TTCAGTCAAATCTATCGTTTTTCCTTGTCTGATGGCACTCTTGTTGCTGCACAAACGAAG
  AGCAAACTCATCCGTTCTCAGACTACTAATGAACCTCAACTTGTAATATCTTTACATATG
  CTTCACAGAGAGCAGAATGTGTGTGTGATGAATCCGGATCTGACTGGACAAACGATGGGG
  AAGCCACTGAATCCAATTAGCTCTAACAGCCCTGCCCATCAGGCCCTGTGCAGTGGGAAC
  CCAGGTCAGGACATGACCCTCAGTAGCAATATAAATTTTCCCATAAATGGCCCAAAGGAA
  CAAATGGGCATGCCCATGGGCAGGTTTGGTGGTTCTGGGGGAATGAACCATGTGTCAGGC
  ATGCAAGCAACCACTCCTCAGGGTAGTAACTATGCACTCAAAATGAACAGCCCCTCACAA
  AGCAGCCCTGGCATGAATCCAGGACAGCCCACCTCCATGCTTTCACCAAGGCATCGCATG
  AGCCCTGGAGTGGCTGGCAGCCCTCGAATCCCACCCAGTCAGTTTTCCCCTGCAGGAAGC
  TTGCATTCCCCTGTGGGAGTTTGCAGCAGCACAGGAAATAGCCATAGTTATACCAACAGC
  TCCCTCAATGCACTTCAGGCCCTCAGCGAGGGGCACGGGGTCTCATTAGGGTCATCGTTG
  GCTTCACCAGACCTAAAAATGGGCAATTTGCAAAACTCCCCAGTTAATATGAATCCTCCC
  CCACTCAGCAAGATGGGAAGCTTGGACTCAAAAGACTGTTTTGGACTATATGGGGAGCCC
  TCTGAAGGTACAACTGGACAAGCAGAGAGCAGCTGCCATCCTGGAGAGCAAAAGGAAACA
  AATGACCCCAACCTGCCCCCGGCCGTGAGCAGTGAGAGAGCTGACGGGCAGAGCAGACTG
  CATGACAGCAAAGGGCAGACCAAACTCCTGCAGCTGCTGACCACCAAATCTGATCAGATG
  GAGCCCTCGCCCTTAGCCAGCTCTTTGTCGGATACAAACAAAGACTCCACAGGTAGCTTG
  CCTGGTTCTGGGTCTACACATGGAACCTCGCTCAAGGAGAAGCATAAAATTTTGCACAGA
  CTCTTGCAGGACAGCAGTTCCCCTGTGGACTTGGCCAAGTTAACAGCAGAAGCCACAGGC
  AAAGACCTGAGCCAGGAGTCCAGCAGCACAGCTCCTGGATCAGAAGTGACTATTAAACAA
  GAGCCGGTGAGCCCCAAGAAGAAAGAGAATGCACTACTTCGCTATTTGCTAGATAAAGAT
  GATACTAAAGATATTGGTTTACCAGAAATAACCCCCAAACTTGAGAGACTGGACAGTAAG
  ACAGATCCTGCCAGTAACACAAAATTAATAGCAATGAAAACTGAGAAGGAGGAGATGAGC
  TTTGAGCCTGGTGACCAGCCTGGCAGTGAGCTGGACAACTTGGAGGAGATTTTGGATGAT
  TTGCAGAATAGTCAATTACCACAGCTTTTCCCAGACACGAGGCCAGGCGCCCCTGCTGGA
  TCAGTTGACAAGCAAGCCATCATCAATGACCTCATGCAACTCACAGCTGAAAACAGCCCT
  GTCACACCTGTTGGAGCCCAGAAAACAGCACTGCGAATTTCACAGAGCACTTTTAATAAC
  CCACGACCAGGGCAACTGGGCAGGTTATTGCCAAACCAGAATTTACCACTTGACATCACA
  TTGCAAAGCCCAACTGGTGCTGGACCTTTCCCACCAATCAGAAACAGTAGTCCCTACTCA
  GTGATACCTCAGCCAGGAATGATGGGTAATCAAGGGATGATAGGAAACCAAGGAAATTTA
  GGGAACAGTAGCACAGGAATGATTGGTAACAGTGCTTCTCGGCCTACTATGCCATCTGGA
  GAATGGGCACCGCAGAGTTCGGCTGTGAGAGTCACCTGTGCTGCTACCACCAGTGCCATG
  AACCGGCCAGTCCAAGGAGGTATGATTCGGAACCCAGCAGCCAGCATCCCCATGAGGCCC
  AGCAGCCAGCCTGGCCAAAGACAGACGCTTCAGTCTCAGGTCATGAATATAGGGCCATCT
  GAATTAGAGATGAACATGGGGGGACCTCAGTATAGCCAACAACAAGCTCCTCCAAATCAG
  ACTGCCCCATGGCCTGAAAGCATCCTGCCTATAGACCAGGCGTCTTTTGCCAGCCAAAAC
  AGGCAGCCATTTGGCAGTTCTCCAGATGACTTGCTATGTCCACATCCTGCAGCTGAGTCT
  CCGAGTGATGAGGGAGCTCTCCTGGACCAGCTGTATCTGGCCTTGCGGAATTTTGATGGC
  CTGGAGGAGATTGATAGAGCCTTAGGAATACCCGAACTGGTCAGCCAGAGCCAAGCAGTA
  GATCCAGAACAGTTCTCAAGTCAGGATTCCAACATCATGCTGGAGCAGAAGGCGCCCGTT
  TTCCCACAGCAGTATGCATCTCAGGCACAAATGGCCCAGGGTAGCTATTCTCCCATGCAA
  GATCCAAACTTTCACACCATGGGACAGCGGCCTAGTTATGCCACACTCCGTATGCAGCCC
  AGACCGGGCCTCAGGCCCACGGGCCTAGTGCAGAACCAGCCAAATCAACTAAGACTTCAA
  CTTCAGCATCGCCTCCAAGCACAGCAGAATCGCCAGCCACTTATGAATCAAATCAGCAAT
  GTTTCCAATGTGAACTTGACTCTGAGGCCTGGAGTACCAACACAGGCACCTATTAATGCA
  CAGATGCTGGCCCAGAGACAGAGGGAAATCCTGAACCAGCATCTTCGACAGAGACAAATG
  CATCAGCAACAGCAAGTTCAGCAACGAACTTTGATGATGAGAGGACAAGGGTTGAATATG
  ACACCAAGCATGGTGGCTCCTAGTGGTATCCCAGCAACTATGAGCAACCCTCGGATTCCC
  CAGGCAAATGCACAGCAGTTTCCATTTCCTCCAAACTACGGAATAAGTCAGCAACCTGAT
  CCAGGCTTTACTGGGGCTACGACTCCCCAGAGCCCACTTATGTCACCCCGAATGGCACAT
  ACACAGAGTCCCATGATGCAACAGTCTCAGGCCAACCCAGCCTATCAGGCCCCCTCCGAC
  ATAAATGGATGGGCGCAGGGGAACATGGGCGGAAACAGCATGTTTTCCCAGCAGTCCCCA
  CCACACTTTGGGCAGCAAGCAAACACCAGCATGTACAGTAACAACATGAACATCAATGTG
  TCCATGGCGACCAACACAGGTGGCATGAGCAGCATGAACCAGATGACAGGACAGATCAGC
  ATGACCTCAGTGACCTCCGTGCCTACGTCAGGGCTGTCCTCCATGGGTCCCGAGCAGGTT
  AATGATCCTGCTCTGAGGGGAGGCAACCTGTTCCCAAACCAGCTGCCTGGAATGGATATG
  ATTAAGCAGGAGGGAGACACAACACGGAAATATTGCTGACACTGCTGAAGCCAGTTGCTT
  CTTCAGCTGACCGGGCTCACTTGCTCAAAACACTTCCAGTCTGGAGAGCTGTGTCTATTT
  GTTTCAACCCAACTGACCTGCCA
  
• Restriction Sites: Please inquire
• ACCN: NM_006540
• Insert Size: 4600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF of NM_006540.2 which changes the protein from a MET to a Ile at 4008bp. OriGene considers this to be a polymorphism and to reflect the natural differences between individuals.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006540.2, NP_006531.1
• RefSeq Size: 6157 bp
• RefSeq ORF: 4395 bp
• Locus ID: 10499
• UniProt ID: Q15596
• Cytogenetics: 8q13.3
• Domains: PAS, HLH
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 all encode isoform a.