ZNF259 (ZPR1) (NM_003904) Human Tagged ORF Clone

CAT#: RG205721

ZNF259 (tGFP-tagged) - Human zinc finger protein 259 (ZNF259)

Product Images

Circular map for RG205721

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF259
• Synonyms: GKAF; ZNF259
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205721 representing NM_003904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCAGCGGGGCTGTGGAACCAGGGCCCCCGGGGGCTGCCGTCGCCCCGTCGCCCGCCCCGGCCC
  CGCCGCCTGCCCCTGATCACCTGTTCCGGCCCATCAGCGCCGAGGACGAGGAGCAGCAGCCCACCGAGAT
  CGAGTCGCTATGCATGAACTGTTACTGCAATGGCATGACGCGCCTCCTGCTCACCAAGATTCCCTTCTTC
  AGAGAAATAATAGTGAGCTCCTTTTCCTGCGAGCACTGTGGCTGGAACAACACGGAGATCCAGTCGGCAG
  GCAGGATCCAGGACCAGGGAGTGCGCTACACTTTGTCTGTCAGGGCTCTGGAGGACATGAACAGAGAAGT
  GGTGAAGACTGACTCTGCTGCCACAAGGATTCCTGAGCTAGATTTTGAAATTCCTGCCTTTAGCCAGAAA
  GGAGCTCTGACCACTGTTGAAGGATTGATCACCCGTGCTATCTCTGGCCTGGAGCAGGACCAGCCTGCAC
  GAAGGGCAAACAAAGATGCTACAGCTGAAAGAATTGATGAGTTCATTGTCAAACTGAAGGAGCTAAAGCA
  AGTAGCCTCCCCTTTCACTCTGATCATTGATGATCCCTCAGGGAACAGTTTTGTGGAAAACCCACATGCT
  CCTCAGAAAGATGATGCCCTGGTGATCACACACTACAACCGGACCCGACAGCAGGAAGAGATGCTGGGGC
  TTCAAGAAGAAGCACCAGCAGAGAAGCCAGAAGAGGAAGATCTCAGAAATGAAGTGCTCCAGTTCAGCAC
  AAACTGCCCAGAATGCAATGCCCCCGCTCAGACCAACATGAAGCTAGTACAAATCCCTCACTTTAAGGAG
  GTTATCATCATGGCTACCAACTGCGAGAACTGTGGGCATCGGACCAATGAGGTGAAATCTGGAGGAGCAG
  TAGAACCCTTGGGCACCAGGATCACCCTCCACATCACAGATGCCTCAGATATGACCAGAGACCTCCTCAA
  GTCTGAGACTTGCAGTGTGGAAATCCCAGAGCTAGAATTTGAACTGGGAATGGCAGTCCTCGGGGGCAAG
  TTCACCACACTGGAAGGGCTGCTGAAAGACATCCGGGAACTGGTGACCAAAAATCCTTTCACACTGGGCG
  ACAGTTCCAATCCTGGACAGACGGAGAGACTACAGGAGTTTAGCCAGAAGATGGACCAGATCATCGAAGG
  TAACATGAAGGCCCACTTTATTATGGATGATCCAGCAGGAAACAGTTACTTGCAGAATGTGTATGCGCCT
  GAAGATGATCCTGAGATGAAGGTGGAGCGTTACAAGCGCACCTTTGACCAAAATGAGGAGCTAGGGCTCA
  ATGACATGAAGACAGAGGGCTATGAGGCAGGCCTGGCTCCGCAACGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205721 representing NM_003904
  Red=Cloning site Green=Tags(s)
  
  MAASGAVEPGPPGAAVAPSPAPAPPPAPDHLFRPISAEDEEQQPTEIESLCMNCYCNGMTRLLLTKIPFF
  REIIVSSFSCEHCGWNNTEIQSAGRIQDQGVRYTLSVRALEDMNREVVKTDSAATRIPELDFEIPAFSQK
  GALTTVEGLITRAISGLEQDQPARRANKDATAERIDEFIVKLKELKQVASPFTLIIDDPSGNSFVENPHA
  PQKDDALVITHYNRTRQQEEMLGLQEEAPAEKPEEEDLRNEVLQFSTNCPECNAPAQTNMKLVQIPHFKE
  VIIMATNCENCGHRTNEVKSGGAVEPLGTRITLHITDASDMTRDLLKSETCSVEIPELEFELGMAVLGGK
  FTTLEGLLKDIRELVTKNPFTLGDSSNPGQTERLQEFSQKMDQIIEGNMKAHFIMDDPAGNSYLQNVYAP
  EDDPEMKVERYKRTFDQNEELGLNDMKTEGYEAGLAPQR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003904
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003904.5
• RefSeq Size: 1810 bp
• RefSeq ORF: 1380 bp
• Locus ID: 8882
• UniProt ID: O75312
• Cytogenetics: 11q23.3
• Domains: Zpr1
• Gene Summary: The protein encoded by this gene is found in the cytoplasm of quiescent cells but translocates to the nucleolus in proliferating cells. The encoded protein interacts with survival motor neuron protein (SMN1) to enhance pre-mRNA splicing and to induce neuronal differentiation and axonal growth. Defects in this gene or the SMN1 gene can cause spinal muscular atrophy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]