PGLS (NM_012088) Human Tagged ORF Clone

CAT#: RG204132

PGLS (tGFP-tagged) - Human 6-phosphogluconolactonase (PGLS)

Product Images

Circular map for RG204132

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PGLS
• Synonyms: 6PGL; HEL-S-304
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204132 representing NM_012088
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGCCGGCCCCGGGCCTCATCTCGGTGTTCTCGAGTTCCCAGGAGCTGGGTGCGGCGCTAGCGC
  AGCTGGTGGCCCAGCGCGCAGCATGCTGCCTGGCAGGGGCCCGCGCCCGTTTCGCGCTCGGCTTGTCGGG
  CGGGAGCCTCGTCTCGATGCTAGCCCGCGAGCTACCCGCCGCCGTCGCCCCTGCCGGGCCAGCTAGCTTA
  GCGCGCTGGACGCTGGGCTTCTGCGACGAGCGCCTCGTGCCCTTCGATCACGCCGAGAGCACGTACGGCC
  TCTACCGGACGCATCTTCTCTCCAGACTGCCGATCCCAGAAAGCCAGGTGATCACCATTAACCCCGAGCT
  GCCTGTGGAGGAGGCGGCTGAGGACTACGCCAAGAAGCTGAGACAGGCATTCCAAGGGGACTCCATCCCG
  GTTTTCGACCTGCTGATCCTGGGGGTGGGCCCCGATGGTCACACCTGCTCACTCTTCCCAGACCACCCCC
  TCCTACAGGAGCGGGAGAAGATTGTGGCTCCCATCAGTGACTCCCCGAAGCCACCGCCACAGCGTGTGAC
  CCTCACGCTACCTGTCCTGAATGCAGCACGAACTGTCATCTTTGTGGCAACTGGAGAAGGCAAGGCAGCT
  GTTCTGAAGCGCATTTTGGAGGACCAGGAGGAAAACCCGCTGCCCGCCGCCCTGGTCCAGCCCCACACCG
  GGAAACTGTGCTGGTTCTTGGACGAGGCGGCCGCCCGCCTCCTGACCGTGCCCTTCGAGAAGCATTCCAC
  TTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204132 representing NM_012088
  Red=Cloning site Green=Tags(s)
  
  MAAPAPGLISVFSSSQELGAALAQLVAQRAACCLAGARARFALGLSGGSLVSMLARELPAAVAPAGPASL
  ARWTLGFCDERLVPFDHAESTYGLYRTHLLSRLPIPESQVITINPELPVEEAAEDYAKKLRQAFQGDSIP
  VFDLLILGVGPDGHTCSLFPDHPLLQEREKIVAPISDSPKPPPQRVTLTLPVLNAARTVIFVATGEGKAA
  VLKRILEDQEENPLPAALVQPHTGKLCWFLDEAAARLLTVPFEKHSTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012088
• ORF Size: 774 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012088.2, NP_036220.1
• RefSeq Size: 1043 bp
• RefSeq ORF: 777 bp
• Locus ID: 25796
• UniProt ID: O95336
• Cytogenetics: 19p13.11
• Domains: Glucosamine_iso
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway
• Gene Summary: Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.[UniProtKB/Swiss-Prot Function]