PGLS (NM_012088) Human Untagged Clone

CAT#: SC115539

PGLS (untagged)-Human 6-phosphogluconolactonase (PGLS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PGLS
• Synonyms: 6PGL; HEL-S-304
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115539 sequence for NM_012088 edited (data generated by NextGen Sequencing)
  ATGGCCGCGCCGGCCCCGGGCCTCATCTCGGTGTTCTCGAGTTCCCAGGAGCTGGGTGCG
  GCGCTAGCGCAGCTGGTGGCCCAGCGCGCAGCATGCTGCCTGGCAGGGGCCCGCGCCCGT
  TTCGCGCTCGGCTTGTCGGGCGGGAGCCTCGTCTCGATGCTAGCCCGCGAGCTACCCGCC
  GCCGTCGCCCCTGCCGGGCCAGCTAGCTTAGCGCGCTGGACGCTGGGCTTCTGCGACGAG
  CGCCTCGTGCCCTTCGATCACGCCGAGAGCACGTACGGCCTCTACCGGACGCATCTTCTC
  TCCAGACTGCCGATCCCAGAAAGCCAGGTGATCACCATTAACCCCGAGCTGCCTGTGGAG
  GAGGCGGCTGAGGACTACGCCAAGAAGCTGAGACAGGCATTCCAAGGGGACTCCATCCCG
  GTTTTCGACCTGCTGATCCTGGGGGTGGGCCCCGATGGTCACACCTGCTCACTCTTCCCA
  GACCACCCCCTCCTACAGGAGCGGGAGAAGATTGTGGCTCCCATCAGTGACTCCCCGAAG
  CCACCGCCACAGCGTGTGACCCTCACGCTACCTGTCCTGAATGCAGCACGAACTGTCATC
  TTTGTGGCAACTGGAGAAGGCAAGGCAGCTGTTCTGAAGCGCATTTTGGAGGACCAGGAG
  GAAAACCCGCTGCCCGCCGCCCTGGTCCAGCCCCACACCGGGAAACTGTGCTGGTTCTTG
  GACGAGGCGGCCGCCCGCCTCCTGACCGTGCCCTTCGAGAAGCATTCCACTTTGTAG
  
  Clone variation with respect to NM_012088.2
  133 c=>t;567 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012088 unedited
  CCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
  TCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTC
  GGCACGAGGCTCGCCATGGCCGCGCCGGCCCCGGGCCTCATCTCGGTGTTCTCGAGTTCC
  CAGGAGCTGGGTGCGGCGCTAGCGCAGCTGGTGGCCCAGCGCGCAGCATGCTGCCTGGCA
  GGGGCCCGCGCCCGTTTCGCGCTCGGCTTGTCGGGCGGGAGCCTCGTCTCGATGCTAGCC
  CGCGAGCTACCCGCCGCCGTCGCCCCTGCCGGGCCAGCTAGCTTAGCGCGCTGGACGCTG
  GGCTTCTGCGACGAGCGCCTCGTGCCCTTCGATCACGCCGAGAGCACGTACGGCCTCTAC
  CGGACGCATCTTCTCTCCAGACTGCCGATCCCAGAAAGCCAGGTGATCACCATTAACCCC
  GAGCTGCCTGTGGAGGAGGCGGCTGAGGACTACGCCAAGAAGCTGAGACAGGCATTCCAA
  GGGGACTCCATCCCGGTTTTCGACCTGCTGATCCTGGGGGTGGGCCCCGATGGTCACACC
  TGCTCACTCTTCCCAGACCACCCCCTCCTACAGGAGCGGGAGAAGACTGTGGCTCCCATC
  AGTGACTCCCCGAAGCCACCGNCACAGCGTGTGACCCTCACGCTACCTGTCCTGAATGCA
  GCACGAACTGTCATCTTTGTGGGCACTGGAGAAGGCANGGCAGCTGNTCTGAAGCGCATT
  TTGGAGGACCAGNNAGAAACCCGCTGCCCGCGCCCTGGTCAGCCCACACGGNAACTGTNG
  CTGGTCTGGACCCGAGCGNCGNCCGCTNCTGACGTGCCCTCGAGAGCATCCACTTTGTAC
  TGNCCNNAGGNACGCGCACTGGNACAGNACGCGCCATGGGGCTGGGCCCTGCTGCCGCCA
  TCTCGGCTTTCTTCAAGNCCGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012088 unedited
  NNTCGTTTTAGCTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTCCAGCAACGCTCCTTTTAATATCCGGATGTCCAG
  AGCCACAGCCTGTGTGTTGAGCCCCGGGTAGGGCTGCTGGCCGGAGGCTGTTGGCTTCCA
  ACAGCAGCACGACGTGGCTTTTTGAAAGGAAAGCCCGGAAAGTGGCGGCCAGCAGGGGCC
  CAGCCCCATGGGCCGCGTGCCTGGTCCCAGCTGCGGCGTCCCTTTGGCCAGCTACAAAGT
  GGAATGCTTCTCGAAGGGCACGGTCAGGAGGCGGGCGGCCGCCTCGTCCAAAAACCAGCA
  CAGTTTCCCGGGGTGGGGCTGGACCAGGGCGGCGGGCAGCGGGTTTTCCTCCTGGTCCTC
  CAAAATGCGCTTCAAAACAGCTGCCTTGCCTTCTCCAGTTGCCACAAAAATGACAGTTCG
  TGCTGCATTTCAGACAGGTAGCGTGAGGGTCACACCCTTGGGCGGGGGGTTCCGGGAGTC
  CCTGATGGGAGCCCCAATCTCCTCCCGCTCCTGTAAGAAGGGGGGGGCCGGGAAAAATGA
  ACAGGGGGTACCATTGGGGCCCCCCCCCAGGAATCAAGGGGCCAAAAACGGGGTGGGAGC
  CCCTTGGAAAGGCCTGCTCAACTTCTTGGGGGAATCCCCCAGCCCCCTCCCTCCCAGGGC
  GCTTCGGGGGTTAAGGGGGAACACCCGGTTTTTTGGGATCCGGCACTTGGAAAAAAAAAA
  CCTCCCCGTAAAGCCCCACCTTCCCTTCGGGGGATCCAAAGGGCCCAAGGCTCTCCTTGA
  AAAAACCCCAACCTTCAAGGGACTATAATATCTTCCCCCGCCGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012088
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012088.2, NP_036220.1
• RefSeq Size: 1043 bp
• RefSeq ORF: 777 bp
• Locus ID: 25796
• UniProt ID: O95336
• Cytogenetics: 19p13.11
• Domains: Glucosamine_iso
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway
• Gene Summary: Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.[UniProtKB/Swiss-Prot Function]