TRPM7 (NM_001301212) Human Tagged ORF Clone

CAT#: RC240218

TRPM7 (myc-DDK-tagged) - Human transient receptor potential cation channel, subfamily M, member 7 (TRPM7), transcript variant 2

Product Images

Circular map for RC240218

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPM7
• Synonyms: ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7; TRP-PLIK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240218 representing NM_001301212
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGAAATCCTGGATAGAAAGCACTTTGACCAAGAGGGAATGTGTATATATTATACCAAGTTCCA
  AGGACCCTCACAGATGCCTTCCAGGATGTCAAATTTGTCAGCAACTCGTCAGGTGTTTTTGTGGTCGCTT
  GGTCAAGCAACATGCTTGTTTTACTGCAAGTCTTGCCATGAAATACTCAGATGTGAAATTGGGTGACCAT
  TTTAATCAGGCAATAGAAGAATGGTCTGTGGAAAAGCATACAGAACAGAGCCCAACGGATGCTTATGGAG
  TCATAAATTTTCAAGGGGGTTCTCATTCCTACAGAGCTAAGTATGTGAGGCTATCATATGACACCAAACC
  TGAAGTCATTCTGCAACTTCTGCTTAAAGAATGGCAAATGGAGTTACCCAAACTTGTTATCTCTGTACAT
  GGGGGCATGCAGAAATTTGAGCTTCACCCACGAATCAAGCAGTTGCTTGGAAAAGGTCTTATTAAAGCTG
  CAGTTACAACTGGAGCCTGGATTTTAACTGGAGGAGTAAACACAGGTGTGGCAAAACATGTTGGAGATGC
  CCTCAAAGAACATGCTTCCAGATCATCTCGAAAGATTTGCACTATCGGAATAGCTCCATGGGGAGTGATT
  GAAAACAGAAATGATCTTGTTGGGAGAGATGTGGTTGCTCCTTATCAAACCTTATTGAACCCCCTGAGCA
  AATTGAATGTTTTGAATAATCTGCATTCCCATTTCATATTGGTGGATGATGGCACTGTTGGAAAGTATGG
  GGCGGAAGTCAGACTGAGAAGAGAACTTGAAAAAACTATTAATCAGCAAAGAATTCATGCTAGGATTGGC
  CAGGGTGTCCCTGTGGTGGCACTTATATTTGAGGGTGGGCCAAATGTTATCCTCACAGTTCTTGAATACC
  TTCAGGAAAGCCCCCCTGTTCCAGTAGTTGTGTGTGAAGGAACAGGCAGAGCTGCAGATCTGCTAGCGTA
  TATTCATAAACAAACAGAAGAAGGAGGGAATCTTCCTGATGCAGCAGAGCCCGATATTATTTCCACTATC
  AAAAAAACATTTAACTTTGGCCAGAATGAAGCACTTCATTTATTTCAAACACTGATGGAGTGCATGAAAA
  GAAAGGAGCTTATCACTGTTTTCCATATTGGGTCAGATGAACATCAAGATATAGATGTAGCAATACTTAC
  TGCACTGCTAAAAGGTACTAATGCATCTGCATTTGACCAGCTTATCCTTACATTGGCATGGGATAGAGTT
  GACATTGCCAAAAATCATGTATTTGTTTATGGACAGCAGTGGCTGGTTGGATCCTTGGAACAAGCTATGC
  TTGATGCTCTTGTAATGGATAGAGTTGCATTTGTAAAACTTCTTATTGAAAATGGAGTAAGCATGCATAA
  ATTCCTTACCATTCCGAGACTGGAAGAACTTTACAACACTAAACAAGGTCCAACTAATCCAATGCTGTTT
  CATCTTGTTCGAGACGTCAAACAGGGAAATCTTCCTCCAGGATATAAGATCACTCTGATTGATATAGGAC
  TTGTTATTGAATATCTCATGGGAGGAACCTACAGATGCACCTATACTAGGAAACGTTTTCGATTAATATA
  TAATAGTCTTGGTGGAAATAATCGGAGGTCTGGCCGAAATACCTCCAGCAGCACTCCTCAGTTGCGAAAG
  AGTCATGAATCTTTTGGCAATAGGGCAGATAAAAAGGAAAAAATGAGGCATAACCATTTCATTAAGACAG
  CACAGCCCTACCGACCAAAGATTGATACAGTTATGGAAGAAGGAAAGAAGAAAAGAACCAAAGATGAAAT
  TGTAGACATTGATGATCCAGAAACCAAGCGCTTTCCTTATCCACTTAATGAACTTTTAATTTGGGCTTGC
  CTTATGAAGAGGCAGGTCATGGCCCGTTTTTTATGGCAACATGGTGAAGAATCAATGGCTAAAGCATTAG
  TTGCCTGTAAGATCTATCGTTCAATGGCATATGAAGCAAAGCAGAGTGACCTGGTAGATGATACTTCAGA
  AGAACTAAAACAGTATTCCAATGATTTTGGTCAGTTGGCCGTTGAATTATTAGAACAGTCCTTCAGACAA
  GATGAAACCATGGCTATGAAATTGCTCACTTATGAACTGAAGAACTGGAGTAATTCAACCTGCCTTAAGT
  TAGCAGTTTCTTCAAGACTTAGACCTTTTGTAGCTCACACCTGTACACAAATGTTGTTATCTGATATGTG
  GATGGGAAGGCTGAATATGAGGAAAAATTCCTGGTACAAGGTCATACTAAGCATTTTAGTTCCACCTGCC
  ATATTGCTGTTAGAGTATAAAACTAAGGCTGAAATGTCCCATATCCCACAATCTCAAGATGCTCATCAGA
  TGACAATGGATGACAGCGAAAACAACTTTCAGAACATAACAGAAGAGATCCCCATGGAAGTGTTTAAAGA
  AGTACGGATTTTGGATAGTAATGAAGGAAAGAATGAGATGGAGATACAAATGAAATCAAAAAAGCTTCCA
  ATTACGCGAAAGTTTTATGCCTTTTATCATGCACCAATTGTAAAATTCTGGTTTAACACGTTGGCATATT
  TAGGATTTCTGATGCTTTATACATTTGTGGTTCTTGTACAAATGGAACAGTTACCTTCAGTTCAAGAATG
  GATTGTTATTGCTTATATTTTTACTTATGCCATTGAGAAAGTCCGTGAGATCTTTATGTCTGAAGCTGGG
  AAAGTAAACCAGAAGATTAAAGTATGGTTTAGTGATTACTTCAACATCAGTGATACAATTGCCATAATTT
  CTTTCTTCATTGGATTTGGACTAAGATTTGGAGCAAAATGGAACTTTGCAAATGCATATGATAATCATGT
  TTTTGTGGCTGGAAGATTAATTTACTGTCTTAACATAATATTTTGGTATGTGCGTTTGCTAGATTTTCTA
  GCTGTAAATCAACAGGCAGGACCTTATGTAATGATGATTGGAAAAATGGTGGCCAATATGTTCTACATTG
  TAGTGATTATGGCTCTTGTATTACTTAGTTTTGGTGTTCCCAGAAAGGCAATACTTTATCCTCATGAAGC
  ACCATCTTGGACTCTTGCTAAAGATATAGTTTTTCACCCATACTGGATGATTTTTGGTGAAGTTTATGCA
  TACGAAATTGATGTGTGTGCAAATGATTCTGTTATCCCTCAAATCTGTGGTCCTGGGACGTGGTTGACTC
  CATTTCTTCAAGCAGTCTACCTCTTTGTACAGTATATCATTATGGTTAATCTTCTTATTGCATTTTTCAA
  CAATGTGTATTTACAAGTGAAGGCAATTTCCAATATTGTATGGAAGTACCAGCGTTATCATTTTATTATG
  GCTTATCATGAGAAACCAGTTCTGCCTCCTCCACTTATCATTCTTAGCCATATAGTTTCTCTGTTTTGCT
  GCATATGTAAGAGAAGAAAGAAAGATAAGACTTCCGATGGACCAAAACTTTTCTTAACAGAAGAAGATCA
  AAAGAAACTTCATGATTTTGAAGAGCAGTGTGTTGAAATGTATTTCAATGAAAAAGATGACAAATTTCAT
  TCTGGGAGTGAAGAGAGAATTCGTGTCACTTTTGAAAGAGTGGAACAGATGTGCATTCAGATTAAAGAAG
  TTGGAGATCGTGTCAACTACATAAAAAGATCATTACAATCATTAGATTCTCAAATTGGCCATTTGCAAGA
  TCTTTCAGCCCTGACGGTAGATACATTAAAAACACTCACTGCCCAGAAAGCGTCGGAAGCTAGCAAAGTT
  CATAATGAAATCACACGAGAACTGAGCATTTCCAAACACTTGGCTCAAAACCTTATTGATGATGGTCCTG
  TAAGACCTTCTGTATGGAAAAAGCATGGTGTTGTAAATACACTTAGCTCCTCTCTTCCTCAAGGTGATCT
  TGAAAGTAATAATCCTTTTCATTGTAATATTTTAATGAAAGATGACAAAGATCCCCAGTGTAATATATTT
  GGTCAAGACTTACCTGCAGTACCCCAGAGAAAAGAATTTAATTTTCCAGAGGCTGGTTCCTCTTCTGGTG
  CCTTATTCCCAAGTGCTGTTTCCCCTCCAGAACTGCGACAGAGACTACATGGGGTAGAACTCTTAAAAAT
  ATTTAATAAAAATCAAAAATTAGGCAGTTCATCTACTAGCATACCACATCTGTCATCCCCACCAACCAAA
  TTTTTTGTTAGTACACCATCTCAGCCAAGTTGCAAAAGCCACTTGGAAACTGGAACCAAAGATCAAGAAA
  CTGTTTGCTCTAAAGCTACAGAAGGAGATAATACAGAATTTGGAGCATTTGTAGGACACAGAGATAGCAT
  GGATTTACAGAGGTTTAAAGAAACATCAAACAAGATAAAAATACTATCCAATAACAATACTTCTGAAAAC
  ACTTTGAAACGAGTGAGTTCTCTTGCTGGATTTACTGACTGTCACAGAACTTCCATTCCTGTTCATTCAA
  AACAAGAAAAAATCAGTAGAAGGCCATCTACCGAAGACACTCATGAAGTAGATTCCAAAGCAGCTTTAAT
  ACCGGATTGGTTACAAGATAGACCATCAAACAGAGAAATGCCATCTGAAGAAGGAACATTAAATGGTCTC
  ACTTCTCCATTTAAGCCAGCTATGGATACAAATTACTATTATTCAGCTGTGGAAAGAAATAACTTGATGA
  GGTTATCACAGAGCATTCCATTTACACCTGTGCCTCCAAGAGGGGAGCCTGTCACAGTGTATCGTTTGGA
  AGAGAGTTCACCCAACATACTAAATAACAGCATGTCTTCTTGGTCACAACTAGGCCTCTGTGCCAAAATA
  GAGTTTTTAAGCAAAGAGGAGATGGGAGGAGGTTTACGAAGAGCTGTCAAAGTACAGTGTACCTGGTCAG
  AACATGATATCCTCAAATCAGGGCATCTTTATATTATCAAATCTTTTCTTCCAGAGGTGGTTAATACATG
  GTCAAGTATTTACAAAGAAGATACAGTTCTGCATCTCTGTCTGAGAGAAATTCAACAACAGAGAGCAGCA
  CAAAAGCTTACGTTTGCCTTTAATCAAATGAAACCCAAATCCATACCATATTCTCCAAGGTTCCTTGAAG
  TTTTCCTGCTGTATTGCCATTCAGCAGGACAGTGGTTTGCTGTGGAAGAATGTATGACTGGAGAATTTAG
  AAAATACAACAATAATAATGGAGATGAGATTATTCCAACTAATACTCTGGAAGAGATCATGCTAGCCTTT
  AGCCACTGGACTTACGAATATACAAGAGGGGAGTTACTGGTACTTGATTTGCAAGGTGTTGGTGAAAATT
  TGACTGACCCATCTGTGATAAAAGCAGAAGAAAAGAGATCCTGTGATATGGTTTTTGGCCCAGCAAATCT
  AGGAGAAGATGCAATTAAAAACTTCAGAGCAAAACATCACTGTAATTCTTGCTGTAGAAAGCTTAAACTT
  CCAGATCTGAAGAGGAATGATTATACGCCTGATAAAATTATATTTCCTCAGGATGAGCCTTCAGATTTGA
  ATCTTCAGCCTGGAAATTCCACCAAAGAATCAGAATCAACTAATTCTGTTCGTCTGATGTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240218 representing NM_001301212
  Red=Cloning site Green=Tags(s)
  
  MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAMKYSDVKLGDH
  FNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEVILQLLLKEWQMELPKLVISVH
  GGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVI
  ENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIG
  QGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTI
  KKTFNFGQNEALHLFQTLMECMKRKELITVFHIGSDEHQDIDVAILTALLKGTNASAFDQLILTLAWDRV
  DIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVAFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLF
  HLVRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRK
  SHESFGNRADKKEKMRHNHFIKTAQPYRPKIDTVMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWAC
  LMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQ
  DETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPA
  ILLLEYKTKAEMSHIPQSQDAHQMTMDDSENNFQNITEEIPMEVFKEVRILDSNEGKNEMEIQMKSKKLP
  ITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKVREIFMSEAG
  KVNQKIKVWFSDYFNISDTIAIISFFIGFGLRFGAKWNFANAYDNHVFVAGRLIYCLNIIFWYVRLLDFL
  AVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEAPSWTLAKDIVFHPYWMIFGEVYA
  YEIDVCANDSVIPQICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIM
  AYHEKPVLPPPLIILSHIVSLFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFNEKDDKFH
  SGSEERIRVTFERVEQMCIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKV
  HNEITRELSISKHLAQNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMKDDKDPQCNIF
  GQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELLKIFNKNQKLGSSSTSIPHLSSPPTK
  FFVSTPSQPSCKSHLETGTKDQETVCSKATEGDNTEFGAFVGHRDSMDLQRFKETSNKIKILSNNNTSEN
  TLKRVSSLAGFTDCHRTSIPVHSKQEKISRRPSTEDTHEVDSKAALIPDWLQDRPSNREMPSEEGTLNGL
  TSPFKPAMDTNYYYSAVERNNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAKI
  EFLSKEEMGGGLRRAVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREIQQQRAA
  QKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAF
  SHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKL
  PDLKRNDYTPDKIIFPQDEPSDLNLQPGNSTKESESTNSVRLML
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301212
• ORF Size: 5592 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301212.2
• RefSeq Size: 10409 bp
• RefSeq ORF: 5595 bp
• Locus ID: 54822
• UniProt ID: Q96QT4
• Cytogenetics: 15q21.2
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Protein Kinase, Transmembrane
• MW: 213.1 kDa
• Gene Summary: This gene belongs to the melastatin subfamily of transient receptor potential family of ion channels. The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. The encoded protein is involved in cytoskeletal organization, cell adhesion, cell migration and organogenesis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam. The gene may also be associated with defects of cardiac function. [provided by RefSeq, Aug 2017]