Phospholipase C beta 2 (PLCB2) (NM_001284298) Human Tagged ORF Clone

CAT#: RC240020

PLCB2 (myc-DDK-tagged) - Human phospholipase C, beta 2 (PLCB2), transcript variant 3

Product Images

Circular map for RC240020

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phospholipase C beta 2
• Synonyms: PLC-beta-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240020 representing NM_001284298
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGCTCAACCCTGTCCTGCTGCCCCCCAAGGTGAAGGCCTATCTGAGCCAAGGGGAGCGCTTCA
  TCAAATGGGATGATGAAACTACAGTTGCCTCTCCAGTTATCCTCCGTGTGGATCCTAAGGGCTACTACTT
  ATACTGGACGTATCAAAGTAAGGAGATGGAGTTTCTGGATATCACCAGCATCCGGGATACTCGCTTTGGG
  AAGTTTGCCAAGATGCCCAAGAGCCAGAAGCTCCGGGACGTCTTCAACATGGACTTTCCTGATAACAGTT
  TCCTGCTGAAGACACTCACGGTGGTGTCCGGCCCGGACATGGTGGACCTCACCTTCCACAACTTCGTCTC
  CTACAAGGAGAACGTGGGCAAGGCCTGGGCTGAGGACGTACTGGCCCTAGTCAAACATCCGCTGACGGCC
  AACGCCTCCCGCAGCACCTTCCTGGACAAGATCCTTGTGAAGCTCAAGATGCAGCTCAACTCTGAAGGGA
  AGATTCCGGTGAAGAACTTTTTCCAGATGTTTCCTGCTGACCGCAAGCGGGTGGAAGCTGCTCTCAGTGC
  CTGCCACCTCCCCAAAGGCAAAAATGACGCCATCAATCCTGAGGACTTCCCAGAACCTGTCTACAAGAGT
  TTCCTCATGAGCCTCTGTCCTCGGCCAGAAATAGATGAGATCTTCACTTCTTACCATGCTAAGGCCAAAC
  CCTACATGACGAAGGAGCACCTGACCAAATTCATCAACCAGAAACAGCGGGACTCCCGGCTTAACTCCCT
  GCTGTTCCCGCCAGCACGGCCTGACCAGGTGCAGGGCCTCATCGACAAGTATGAGCCCAGTGGCATCAAT
  GCACAGAGGGGCCAGCTGTCACCTGAAGGCATGGTCTGGTTTCTCTGTGGGCCAGAGAACAGCGTGCTGG
  CCCAGGACAAGCTGCTGCTCCACCACGACATGACGCAGCCACTCAATCATTACTTCATCAACTCGTCCCA
  CAACACCTACCTGACAGCCGGCCAGTTCTCAGGCCTCTCCTCGGCTGAGATGTACCGCCAGGTGCTGCTC
  TCTGGCTGCCGTTGCGTGGAGCTAGACTGCTGGAAGGGGAAACCCCCTGACGAGGAGCCCATTATCACCC
  ATGGCTTCACCATGACCACAGACATCTTCTTCAAAGAAGCAATTGAGGCTATTGCAGAAAGCGCCTTTAA
  GACCTCCCCCTATCCCATCATCCTGTCGTTTGAGAACCATGTGGACTCACCCCGCCAGCAGGCTAAGATG
  GCTGAGTATTGCCGGACGATCTTTGGGGATATGCTGCTCACAGAGCCCCTGGAAAAGTTCCCACTAAAAC
  CAGGTGTCCCCCTGCCCAGCCCTGAGGATCTCAGGGGCAAGATCCTCATCAAGAACAAGAAGAACCAGTT
  TTCTGGCCCCACCTCCTCCAGTAAGGATACTGGTGGGGAGGCTGAGGGCAGCAGCCCACCCAGTGCCCCT
  GCAGGTGAGGGCACAGTGTGGGCTGGCGAGGAAGGGACTGAGCTGGAGGAGGAGGAGGTGGAAGAGGAAG
  AGGAGGAGGAGTCAGGAAACCTGGATGAAGAAGAGATTAAGAAGATGCAGTCGGATGAGGGCACAGCGGG
  CCTGGAAGTGACGGCTTATGAGGAGATGTCCAGCCTAGTCAATTACATCCAGCCCACCAAGTTCGTCTCC
  TTTGAGTTCTCTGCCCAAAAGAACCGAAGTTATGTCATCTCGTCCTTCACAGAGCTCAAGGCATATGACC
  TGCTCTCCAAGGCCTCGGTGCAGTTTGTGGACTACAACAAGCGCCAGATGAGCCGCATTTACCCCAAGGG
  AACCCGCATGGACTCCTCCAACTACATGCCCCAGATGTTCTGGAATGCTGGATGCCAGATGGTTGCCCTC
  AACTTCCAGACGATGGACTTGCCCATGCAGCAGAACATGGCAGTATTTGAGTTCAACGGGCAGAGCGGCT
  ACCTCCTCAAGCATGAGTTCATGCGCCGGCCGGACAAGCAGTTCAACCCCTTCTCAGTGGACCGCATCGA
  CGTGGTGGTGGCCACCACCCTTTCCATTACGGTGATCTCTGGGCAGTTCCTGTCAGAACGCAGCGTGCGC
  ACCTATGTAGAAGTGGAGCTGTTTGGCCTTCCTGGGGACCCCAAGAGGCGCTATCGAACTAAGCTGTCAC
  CCAGTACTAACTCCATCAATCCTGTCTGGAAGGAGGAGCCCTTTGTCTTTGAGAAGATCTTGATGCCTGA
  GCTGGCCTCCCTCAGAGTGGCTGTGATGGAGGAAGGCAACAAGTTTCTTGGACACCGCATCATCCCCATC
  AATGCCCTAAATTCTGGGTACCACCACCTGTGCCTGCACAGTGAGAGCAACATGCCCCTCACCATGCCTG
  CGCTCTTCATCTTCCTGGAGATGAAGGACTACATACCTGGTGCTTGGGCAGATCTCACTGTGGCCCTCGC
  CAACCCCATTAAGTTCTTCAGTGCCCATGACACGAAGTCTGTGAAGCTCAAGGAGGCCATGGGAGGTCTG
  CCTGAGAAGCCCTTCCCACTGGCGAGTCCAGTTGCCAGCCAGGTCAATGGGGCGTTGGCCCCAACGAGCA
  ATGGGTCACCAGAGCCGCGGACCGCCAGCCTGGAGGAGCTCCGGGAGCTAAAGGGCGTGGTGAAGCTGCA
  GCGGCGGCACGAGAAGGAGCTGCGAGAGTTGGAGCGGCGCGGAGCGCGGCGCTGGGAGGAGCTGCTGCAG
  CGGGGCGCGGCGCAGCTGGCGGAGCTCGGGCCACCGGGCGTGGGGGGCGTCGGGGCCTGCAAGCTCGGTC
  CCGGCAAGGGCTCTCGCAAGAAGAGGAGCCTGCCCCGCGAGGAGAGCGCCGGAGCCGCGCCGGGCGAGGG
  CCCTGAGGGCGTGGACGGGCGCGTGCGGGAGCTGAAAGACAGGCTGGAGCTGGAGCTGCTGCGGCAGGGC
  GAGGAGCAGTACGAGTGCGTTCTGAAGCGCAAGGAGCAGCACGTGGCCGAGCAAATCTCCAAAATGATGG
  AGCTGGCCAGAGAGAAACAGGCGGCAGAGCTGAAGGCCCTGAAGGAGACGTCGGAGAACGACACCAAAGA
  GATGAAGAAAAAGCTGGAGACAAAGAGACTGGAGCGGATCCAGGGCATGACCAAAGTCACCACAGACAAG
  ATGGCCCAGGAGAGGTTGAAGAGAGAGATTAACAACTCCCACATCCAGGAAGTAGTGCAGGTGATCAAGC
  AGATGACGGAGAACTTGGAGAGGCACCAGGAGAAGCTGGAGGAGAAGCAGGCGGCTTGCCTGGAACAGAT
  ACGGGAGATGGAAAAGCAGTTCCAGAAGGAGGCGCTGGCAGAGTACGAGGCCAGGATGAAGGGTCTGGAG
  GCAGAGGTGAAGGAGTCGGTGAGGGCCTGCCTCAGGACCTGCTTTCCCTCCGAGGCCAAGGACAAGCCTG
  AGAGGGCCTGCGAGTGCCCCCCAGAGCTGTGTGAGCAGGACCCACTCATAGCAAAGGCAGATGCCCAGGA
  GAGCCGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240020 representing NM_001284298
  Red=Cloning site Green=Tags(s)
  
  MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFG
  KFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTA
  NASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKS
  FLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGIN
  AQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLL
  SGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKM
  AEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAP
  AGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVS
  FEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVAL
  NFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVR
  TYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPI
  NALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGL
  PEKPFPLASPVASQVNGALAPTSNGSPEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQ
  RGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQG
  EEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDK
  MAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLE
  AEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001284298
• ORF Size: 3510 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284298.2
• RefSeq Size: 4649 bp
• RefSeq ORF: 3513 bp
• Locus ID: 5330
• UniProt ID: Q00722
• Cytogenetics: 15q15.1
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Taste transduction, Vascular smooth muscle contraction, Wnt signaling pathway
• MW: 133 kDa
• Gene Summary: The protein encoded by this gene is a phosphodiesterase that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. The encoded protein is activated by G proteins and has been shown to be involved in the type 2 taste receptor signal transduction pathway. In addition, nuclear factor kappa B can regulate the transcription of this gene, whose protein product is also an important regulator of platelet responses. [provided by RefSeq, Jan 2017]