FAM120A (NM_001286722) Human Tagged ORF Clone

CAT#: RC239981

FAM120A (myc-DDK-tagged) - Human family with sequence similarity 120A (FAM120A), transcript variant 2

Product Images

Circular map for RC239981

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM120A
• Synonyms: C9orf10; HBVPTPAP; OSSA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239981 representing NM_001286722
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGTGCAGGGCTTCCAGGACTACATCGAGAAGCACTGCCCGAGCGCCGTGGTGCCGGTGGAGCTGC
  AGAAGCTGGCCCGGGGCAGCCTGGTGGGCGGCGGGCGGCAGCGGCCCCCGCAGACCCCGCTGCGCCTGCT
  GGTGGACGCCGACAACTGCCTGCACCGCCTCTACGGCGGCTTCTACACCGACTGGGTCAGCGGCGGCCAG
  TGGAACCACATGCTTGGCTACCTGGCGGCGCTGGCCAAGGCCTGCTTCGGCGGCAACATCGAGCTCTTCG
  TCTTCTTCAACGGCGCGCTCGAGAAGGCCCGGCTGCACGAGTGGGTCAAGCGGCAGGGCAACGAGCGCCA
  GACGGCACAGCAGATCGTCAGCCATGTCCAGAACAAGGGCACCCCGCCGCCAAAGGTCTGGTTCCTGCCG
  CCCGTCTGCATGGCCCACTGCATCCGCCTGGCGCTCATCCGCTTCCACGTCAAGGTTGCACAGAGCATTG
  AGGATCACCATCAGGAAGTGATTGGTTTCTGCAGAGAGAATGGTTTCCATGGCTTGGTTGCGTATGACTC
  TGATTATGCACTGTGCAACATCCCCTACTATTTCAGTGCCCATGCCCTAAAACTGAGCCGGAACGGGAAA
  AGTCTCACCACAAGCCAATATCTGATGCATGAAGTTGCCAAGCAACTGGACCTGAATCCAAATCGTTTTC
  CTATTTTTGCTGCTCTCTTAGGAAATCACATTCTGCCTGATGAAGATCTGGCTTCCTTTCACTGGAGTTT
  ACTTGGTCCAGAACATCCACTAGCCTCACTAAAGGTCCGGGCCCACCAGCTGGTCTTGCCACCTTGCGAC
  GTAGTGATCAAAGCCGTTGCTGACTATGTACGCAACATTCAGGACACCTCTGACTTGGATGCCATAGCTA
  AAGATGTTTTCCAGCATTCACAGTCTAGAACAGATGACAAAGTTATTCGATTTAAGAGAGCAATTGGATA
  TTATTCAGCGACTAGTAAGCCTATGTCATTTCATCCACCACATTACTTAGCCAGACCCGGTCCGTTTGGA
  ATGCCTGGGATGGTGCCGCCGCATGTTCCTCCTCAGATGCTCAACATTCCGCAGACCTCTCTGCAAGCAA
  AGCCCGTGGCCCCACAGGTGCCCAGCCCAGGGGGCGCCCCGGGCCAGGGTCCATACCCGTACAGCCTCTC
  TGAGCCAGCACCTCTCACTTTGGACACGAGCGGGAAGAATCTGACGGAGCAGAACAGCTACAGCAACATT
  CCTCACGAAGGGAAGCACACGCCGCTGTATGAGCGGTCCTCGCCCATCAACCCGGCCCAGAGCGGCAGCC
  CCAACCACGTGGATTCCGCCTACTTCCCTGGCTCTTCTACATCGTCATCTTCCGACAACGACGAGGGCAG
  CGGAGGGGCGACAAACCATATCAGCGGGAACAAGATTGGCTGGGAGAAGACGGGAAGCCACTCAGAGCCT
  CAGGCACGAGGAGACCCAGGAGACCAAACAAAGGCAGAAGGCTCGTCCACTGCCTCTTCAGGAAGCCAAC
  TAGCCGAAGGCAAGGGAAGCCAGATGGGCACTGTCCAGCCAATCCCGTGCCTCCTGTCGATGCCCACCAG
  GAACCACATGGACATCACCACACCTCCCCTGCCCCCCGTCGCACCTGAGGTGCTGAGAGTGGCCGAGCAC
  AGGCACAAGAAGGGGCTGATGTACCCCTACATCTTCCATGTCCTGACGAAGGGTGAAATCAAAATTGCTG
  TTTCTATTGAAGATGAAGCCAACAAGGACCTGCCTCCGGCCGCTCTGCTCTATAGGCCAGTTCGTCAGTA
  TGTTTACGGAGTCCTGTTTAGTTTGGCAGAAAGCAGAAAGAAAACTGAGAGACTTGCTTTTAGAAAGAAC
  AGACTTCCACCAGAATTTTCACCAGTGATCATTAAAGAATGGGCAGCTTACAAAGGAAAGTCTCCTCAAA
  CCCCGGAACTGGTTGAAGCTCTTGCCTTCAGGGAGTGGACCTGCCCCAACCTGAAGAGGCTGTGGTTGGG
  TAAGGCGGTAGAGGACAAGAACCGCAGGATGAGGGCCTTCCTGGCCTGCATGAGGTCGGACACCCCAGCC
  ATGCTCAACCCTGCCAACGTGCCCACTCACCTCATGGTGCTCTGCTGCGTCTTACGGTACATGGTGCAGT
  GGCCGGGAGCACGCATCCTTCGGCGTCAGGAGCTAGATGCCTTCCTGGCTCAGGCGCTGTCCCCCAAACT
  CTACGAGCCTGATCAGCTCCAGGAGCTCAAGATTGAGAACCTAGATCCCCGAGGAATTCAGCTATCAGCT
  CTCTTCATGAGTGGAGTAGACATGGCCTTGTTTGCAAATGATGCATGCGGACAGCCAATCCCCTGGGAAC
  ACTGTTGTCCTTGGATGTATTTTGATGGGAAGCTCTTCCAATCCAAACTCCTCAAAGCCAGCCGGGAAAA
  GACCCCACTCATTGACCTCTGTGATGGTCAGGCTGATCAGGCTGCCAAGGTAGAGAAGATGCGCCAGAGC
  GTCCTCGAGGGGCTCAGCTTCTCCAGGCAGAGCCACACGCTCCCTTTCCCGCCGCCACCTGCCCTGCCCT
  TCTACCCTGCCTCTGCGTACCCCCGGCACTTTGGGCCTGTCCCACCCTCTCAGGGCAGGGGCAGAGGCTT
  TGCAGGCGTCTGTGGCTTTGGAGGCCCCTATGGGGAAACGGTAGCAACAGGCCCTTACCGTGCCTTCCGT
  GTGGCGGCAGCATCGGGACACTGCGGAGCCTTCTCAGGCAGTGACAGCAGCAGGACTAGCAAGTCCCAGG
  GCGGAGTCCAACCTATACCTTCTCAGGGAGGCAAACTAGAAATAGCTGGCACTGTGGTTGGCCATTGGGC
  TGGGAGCAGGCGGGGCCGTGGGGGCCGGGGGCCTTTCCCCCTGCAGGTGGTTTCTGTCGGAGGACCAGCT
  AGAGGGCGTCCAAGAGGAGTTATTTCCACCCCAGTGATTAGAACATTTGGAAGAGGTGGAAGGTACTATG
  GCAGAGGTTACAAAAACCAGGCAGCAATTCAGGGCAGACCTCCTTATGCTGCTTCAGCAGAAGAAGTGGC
  CAAAGAACTTAAGTCAAAATCTGGGGAATCGAAGTCCTCTGCTATGTCTTCAGACGGGTCCCTGGCTGAA
  AACGGAGTGATGGCCGAGGAGAAGCCGGCTCCCCAGATGAACGGGAGCACGGGTGACGCCAGGGCCCCCA
  GCCACTCTGAAAGTGCCTTGAATAATGACTCTAAAACGTGCAATACAAATCCTCATTTAAATGCACTAAG
  TACAGACAGCGCTTGCCGCAGAGAAGCTGCTCTGGAGGCAGCTGTCTTAAATAAAGAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239981 representing NM_001286722
  Red=Cloning site Green=Tags(s)
  
  MGVQGFQDYIEKHCPSAVVPVELQKLARGSLVGGGRQRPPQTPLRLLVDADNCLHRLYGGFYTDWVSGGQ
  WNHMLGYLAALAKACFGGNIELFVFFNGALEKARLHEWVKRQGNERQTAQQIVSHVQNKGTPPPKVWFLP
  PVCMAHCIRLALIRFHVKVAQSIEDHHQEVIGFCRENGFHGLVAYDSDYALCNIPYYFSAHALKLSRNGK
  SLTTSQYLMHEVAKQLDLNPNRFPIFAALLGNHILPDEDLASFHWSLLGPEHPLASLKVRAHQLVLPPCD
  VVIKAVADYVRNIQDTSDLDAIAKDVFQHSQSRTDDKVIRFKRAIGYYSATSKPMSFHPPHYLARPGPFG
  MPGMVPPHVPPQMLNIPQTSLQAKPVAPQVPSPGGAPGQGPYPYSLSEPAPLTLDTSGKNLTEQNSYSNI
  PHEGKHTPLYERSSPINPAQSGSPNHVDSAYFPGSSTSSSSDNDEGSGGATNHISGNKIGWEKTGSHSEP
  QARGDPGDQTKAEGSSTASSGSQLAEGKGSQMGTVQPIPCLLSMPTRNHMDITTPPLPPVAPEVLRVAEH
  RHKKGLMYPYIFHVLTKGEIKIAVSIEDEANKDLPPAALLYRPVRQYVYGVLFSLAESRKKTERLAFRKN
  RLPPEFSPVIIKEWAAYKGKSPQTPELVEALAFREWTCPNLKRLWLGKAVEDKNRRMRAFLACMRSDTPA
  MLNPANVPTHLMVLCCVLRYMVQWPGARILRRQELDAFLAQALSPKLYEPDQLQELKIENLDPRGIQLSA
  LFMSGVDMALFANDACGQPIPWEHCCPWMYFDGKLFQSKLLKASREKTPLIDLCDGQADQAAKVEKMRQS
  VLEGLSFSRQSHTLPFPPPPALPFYPASAYPRHFGPVPPSQGRGRGFAGVCGFGGPYGETVATGPYRAFR
  VAAASGHCGAFSGSDSSRTSKSQGGVQPIPSQGGKLEIAGTVVGHWAGSRRGRGGRGPFPLQVVSVGGPA
  RGRPRGVISTPVIRTFGRGGRYYGRGYKNQAAIQGRPPYAASAEEVAKELKSKSGESKSSAMSSDGSLAE
  NGVMAEEKPAPQMNGSTGDARAPSHSESALNNDSKTCNTNPHLNALSTDSACRREAALEAAVLNKEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286722
• ORF Size: 3351 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286722.2
• RefSeq Size: 5161 bp
• RefSeq ORF: 3354 bp
• Locus ID: 23196
• UniProt ID: Q9NZB2
• Cytogenetics: 9q22.31
• MW: 122.3 kDa
• Gene Summary: May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.[UniProtKB/Swiss-Prot Function]