SENP7 (NM_001282803) Human Tagged ORF Clone

CAT#: RC239736

SENP7 (myc-DDK-tagged) - Human SUMO1/sentrin specific peptidase 7 (SENP7), transcript variant 5

Product Images

Circular map for RC239736

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SENP7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239736 representing NM_001282803
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAAATGCAAAACCAGAGGATGTCCATGTTCAATCACCACTGTCCAAATTCAGAAGCTCAGAACGCT
  GGACTCTCCCTTTGCAGTGGGAAAGAAGCCTAAGGAATAAAGTCATCTCTCTAGACCATAAAAATAAAAA
  ACATATCCGAGGGTGTCCTGTTACTTCCAAGTCATCACCAGAAAGGGGCTCACAACGAAGTAAGACAGTA
  GATGACAATTCTGCAAAGCAGACTGCGCACAATAAAGAAAAACGAAGAAAGGATGATGGCATTTCTCTTT
  TAATATCTGATACTCAGCCTGAAGACCTTAACAGTGGAAGTAGAGGTTGTGATCATCTCGAACAGGAAAG
  CAGAAACAAGGATGTTAAATATTCTGATTCAAAAGTGGAACTCACTCTGATTTCCAGGAAGACAAAGAGA
  AGGCTTAGAAATAATTTACCTGATTCTCAATATTGTACTTCTTTGGATAAGTCAACAGAACAGACAAAAA
  AACAAGAAGATGACTCAACAATATCCACTGAGTTTGAAAAGCCAAGTGAAAACTATCATCAGGATCCAAA
  ACTGCCTGAAGAAATTACAACTAAACCTACAAAAAGTGATTTTACTAAACTATCCTCACTTAACAGTCAG
  GAGTTGACTTTGAGTAATGCCACCAAAAGTGCCTCTGCCGGTTCAACCACTGAAACCGTTGAGAACTCTA
  ATTCCATTGATATTGTGGGGATTTCTTCCCTGGTTGAGAAGGATGAGAATGAGTTGAATACCATAGAAAA
  GCCTATTCTAAGAGGACATAATGAAGGGAACCAATCACTGATCTCAGCTGAACCAATTGTTGTTTCCAGT
  GATGAAGAAGGACCTGTTGAACATAAAAGTTCAGAAATTCTTAAGTTACAATCTAAGCAAGACCGTGAGA
  CAACTAATGAAAATGAGAGTACTTCTGAATCAGCATTGTTAGAACTACCATTGATTACATGTGAATCTGT
  ACAGATGTCATCTGAATTATGCCCATATAATCCTGTCATGGAGAACATTTCCAGTATTATGCCTAGTAAT
  GAGATGGATCTACAACTGGATTTTATATTTACTTCTGTTTATATTGGTAAAATAAAAGGAGCTTCTAAAG
  GTTGTGTTACAATCACAAAAAAATATATTAAGATCCCATTTCAAGTGTCCCTGAATGAGATTTCATTGCT
  AGTGGATACCACACATTTAAAGCGGTTTGGGTTATGGAAAAGTAAGGATGATAATCACAGTAAAAGGAGT
  CATGCTATTCTTTTCTTCTGGGTCTCTTCAGATTATCTTCAAGAGATTCAGACCCAATTAGAACACTCTG
  TATTAAGCCAGCAATCAAAATCTAGTGAATTCATTTTCCTTGAACTACACAATCCTGTTTCACAAAGAGA
  AGAATTGAAGCTGAAAGATATTATGACGGAAATAAGTATAATCAGTGGAGAATTAGAGCTTTCTTACCCG
  TTGTCTTGGGTTCAGGCATTTCCTTTGTTTCAGAACCTCTCTTCAAAAGAAAGTTCTTTTATTCATTATT
  ACTGTGTTTCAACTTGTTCTTTCCCTGCTGGTGTTGCTGTTGCTGAAGAAATGAAGCTGAAATCAGTATC
  TCAGCCCTCAAACACAGATGCGGCCAAGCCTACTTACACCTTCCTGCAGAAGCAAAGTAGCGGTTGCTAC
  TCCCTTTCTATTACATCTAATCCAGATGAAGAATGGCGAGAAGTCAGGCACACTGGACTTGTTCAGAAGT
  TGATTGTATATCCTCCACCACCTACTAAGGGGGGATTAGGAGTAACTAATGAAGATCTGGAGTGTTTAGA
  AGAAGGAGAGTTTCTTAATGATGTAATCATTGATTTTTACCTTAAGTATCTTATATTGGAGAAGGCATCA
  GATGAACTTGTTGAACGAAGTCACATTTTTAGTAGCTTTTTCTATAAATGCTTGACAAGAAAGGAAAATA
  ATTTAACAGAAGATAATCCAAATCTTTCAATGGCACAGAGAAGACATAAAAGAGTAAGAACATGGACTCG
  TCACATAAACATTTTTAATAAAGATTACATCTTTGTACCTGTAAATGAGTCGTCTCACTGGTATCTCGCA
  GTCATTTGTTTTCCATGGTTAGAAGAAGCTGTGTATGAAGATTTTCCACAAACTGTATCCCAGCAGTCCC
  AGGCTCAGCAGTCCCAAAATGACAACAAAACAATAGATAATGATCTACGTACTACTTCGACACTGTCTTT
  GAGTGCAGAGGATTCCCAAAGTACCGAGTCGAATATGTCAGTACCAAAGAAAATGTGTAAAAGGCCATGT
  ATTCTTATACTAGACTCCTTGAAAGCTGCTTCTGTACAAAACACAGTTCAGAATTTACGAGAGTATTTAG
  AGGTAGAGTGGGAAGTTAAACTAAAAACTCATCGTCAATTCAGCAAAACAAACATGGTGGATCTATGCCC
  TAAAGTTCCTAAACAGGACAATAGCAGTGATTGTGGAGTATATTTATTGCAGTATGTGGAAAGCTTCTTC
  AAGGATCCTATTGTTAACTTTGAACTTCCAATTCATTTGGAGAAGTGGTTTCCTCGTCATGTAATAAAGA
  CCAAACGGGAAGATATTCGAGAGCTCATCTTGAAACTTCATTTACAGCAACAGAAGGGCAGCAGTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239736 representing NM_001282803
  Red=Cloning site Green=Tags(s)
  
  MLNAKPEDVHVQSPLSKFRSSERWTLPLQWERSLRNKVISLDHKNKKHIRGCPVTSKSSPERGSQRSKTV
  DDNSAKQTAHNKEKRRKDDGISLLISDTQPEDLNSGSRGCDHLEQESRNKDVKYSDSKVELTLISRKTKR
  RLRNNLPDSQYCTSLDKSTEQTKKQEDDSTISTEFEKPSENYHQDPKLPEEITTKPTKSDFTKLSSLNSQ
  ELTLSNATKSASAGSTTETVENSNSIDIVGISSLVEKDENELNTIEKPILRGHNEGNQSLISAEPIVVSS
  DEEGPVEHKSSEILKLQSKQDRETTNENESTSESALLELPLITCESVQMSSELCPYNPVMENISSIMPSN
  EMDLQLDFIFTSVYIGKIKGASKGCVTITKKYIKIPFQVSLNEISLLVDTTHLKRFGLWKSKDDNHSKRS
  HAILFFWVSSDYLQEIQTQLEHSVLSQQSKSSEFIFLELHNPVSQREELKLKDIMTEISIISGELELSYP
  LSWVQAFPLFQNLSSKESSFIHYYCVSTCSFPAGVAVAEEMKLKSVSQPSNTDAAKPTYTFLQKQSSGCY
  SLSITSNPDEEWREVRHTGLVQKLIVYPPPPTKGGLGVTNEDLECLEEGEFLNDVIIDFYLKYLILEKAS
  DELVERSHIFSSFFYKCLTRKENNLTEDNPNLSMAQRRHKRVRTWTRHINIFNKDYIFVPVNESSHWYLA
  VICFPWLEEAVYEDFPQTVSQQSQAQQSQNDNKTIDNDLRTTSTLSLSAEDSQSTESNMSVPKKMCKRPC
  ILILDSLKAASVQNTVQNLREYLEVEWEVKLKTHRQFSKTNMVDLCPKVPKQDNSSDCGVYLLQYVESFF
  KDPIVNFELPIHLEKWFPRHVIKTKREDIRELILKLHLQQQKGSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282803
• ORF Size: 2658 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282803.2
• RefSeq Size: 4575 bp
• RefSeq ORF: 2661 bp
• Locus ID: 57337
• UniProt ID: Q9BQF6
• Cytogenetics: 3q12.3
• Protein Families: Druggable Genome, Protease
• MW: 101.6 kDa
• Gene Summary: The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for many cellular processes. SUMO-specific proteases, such as SENP7, process SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 [PubMed 18799455]).[supplied by OMIM, Jun 2009]