ZNF227 (NM_001289166) Human Tagged ORF Clone

CAT#: RC239591

ZNF227 (myc-DDK-tagged) - Human zinc finger protein 227 (ZNF227), transcript variant 1

Product Images

Circular map for RC239591

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF227
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239591 representing NM_001289166
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATCTCAGAACTATGACCTTCCCCAGAAGAAGCAGGAGAAAATGACCAAGTTTCAGGAGGCTGTGA
  CATTCAAGGATGTGGCTGTGGTCTTCTCCAGGGAGGAACTGCGACTGCTCGATCTTACCCAGAGGAAGCT
  GTACCGAGATGTCATGGTGGAGAACTTCAAGAACCTGGTTGCAGTGGGGCATCTTCCCTTCCAACCAGAT
  ATGGTATCCCAATTGGAAGCAGAAGAAAAGCTTTGGATGATGGAAACAGAAACCCAAAGAAGCAGCAAGC
  ATCAAAATAAGATGGAAACACTCCAAAAATTTGCATTAAAATACCTTTCAAATCAAGAGCTGTCCTGCTG
  GCAAATCTGGAAACAGGTTGCAAGTGAATTAACCAGGTGTCTTCAGGGGAAGAGTTCCCAGTTATTACAA
  GGTGACTCTATTCAGGTTTCTGAAAATGAGAACAATATAATGAACCCTAAAGGAGATAGCTCTATTTATA
  TTGAAAATCAAGAGTTTCCATTTTGGAGAACCCAGCATTCTTGCGGGAATACATATCTGAGTGAGTCACA
  GATTCAGAGTAGAGGTAAGCAAATTGATGTGAAAAATAACCTGCAAATACATGAAGACTTCATGAAGAAA
  TCACCATTTCATGAGCATATTAAAACTGACACAGAACCAAAACCCTGCAAAGGTAATGAATATGGCAAAA
  TCATTAGTGATGGCTCCAATCAGAAATTACCCTTAGGAGAGAAACCCCATCCATGTGGTGAGTGTGGAAG
  GGGCTTCAGTTATAGCCCAAGGCTTCCCCTTCATCCGAATGTTCATACAGGAGAAAAATGCTTCAGTCAA
  AGCTCACATCTGCGAACTCATCAGAGAATTCACCCAGGAGAGAAACTCAATAGATGTCATGAATCTGGTG
  ATTGCTTCAATAAGAGCTCTTTTCATTCTTATCAATCTAATCATACAGGAGAGAAGTCTTATAGATGCGA
  CAGTTGCGGCAAGGGATTCAGTAGCAGCACGGGTCTTATCATTCATTACAGAACTCATACTGGAGAGAAA
  CCCTATAAATGCGAGGAATGTGGTAAATGCTTTAGTCAAAGTTCAAATTTTCAGTGCCATCAGAGAGTCC
  ACACTGAAGAAAAACCATACAAATGCGAAGAGTGTGGTAAGGGCTTCGGTTGGAGTGTTAATCTCCGTGT
  TCACCAGAGGGTCCACAGGGGTGAGAAGCCCTATAAATGTGAGGAATGTGGTAAGGGCTTCACTCAGGCT
  GCACATTTTCACATCCATCAGAGAGTCCACACTGGAGAGAAACCCTACAAGTGTGATGTGTGTGGTAAGG
  GCTTCAGCCACAATTCACCATTAATATGCCATCGGAGAGTCCACACAGGAGAGAAGCCATACAAGTGTGA
  GGCGTGTGGGAAAGGCTTTACCCGTAATACAGATCTGCATATTCATTTCAGAGTTCACACGGGAGAGAAA
  CCCTATAAATGTAAGGAGTGTGGTAAGGGCTTCAGTCAGGCTTCAAATCTTCAAGTCCATCAGAATGTCC
  ACACTGGGGAGAAACGATTCAAGTGTGAAACGTGTGGGAAGGGCTTCAGTCAGTCCTCAAAGCTTCAAAC
  CCATCAGCGAGTCCACACTGGAGAGAAACCATATAGATGTGATGTGTGTGGTAAGGACTTCAGTTATAGT
  TCAAATCTTAAACTACACCAAGTAATTCACACTGGAGAAAAACCATATAAATGTGAGGAATGTGGGAAGG
  GCTTCAGTTGGAGATCAAATCTTCATGCACATCAAAGAGTTCACTCAGGAGAAAAACCCTATAAATGTGA
  GCAGTGTGATAAGAGCTTCAGTCAGGCCATAGATTTTCGGGTACATCAGAGAGTCCATACTGGAGAGAAG
  CCATACAAATGTGGTGTCTGTGGTAAGGGCTTCAGTCAGTCCTCTGGTCTTCAATCCCATCAGAGAGTCC
  ACACGGGGGAAAAGCCATACAAATGTGATGTGTGTGGAAAGGGCTTTAGATACAGTTCGCAGTTTATATA
  CCATCAGAGAGGCCACACTGGAGAAAAACCTTACAAATGTGAAGAGTGTGGGAAAGGCTTTGGTAGGAGC
  TTGAATCTTCGCCATCATCAGAGGGTCCACACGGGAGAGAAACCCCATATATGTGAGGAGTGTGGTAAGG
  CCTTCAGTCTCCCCTCAAATCTTCGAGTCCACCTGGGTGTTCACACCAGGGAAAAACTCTTTAAATGTGA
  AGAGTGTGGTAAAGGCTTCAGTCAGAGTGCACGTCTTGAAGCCCATCAGAGAGTCCACACTGGAGAAAAA
  CCATACAAATGTGACATATGTGATAAGGACTTCCGTCACCGTTCACGTCTTACATATCATCAGAAAGTCC
  ATACTGGTAAAAAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239591 representing NM_001289166
  Red=Cloning site Green=Tags(s)
  
  MPSQNYDLPQKKQEKMTKFQEAVTFKDVAVVFSREELRLLDLTQRKLYRDVMVENFKNLVAVGHLPFQPD
  MVSQLEAEEKLWMMETETQRSSKHQNKMETLQKFALKYLSNQELSCWQIWKQVASELTRCLQGKSSQLLQ
  GDSIQVSENENNIMNPKGDSSIYIENQEFPFWRTQHSCGNTYLSESQIQSRGKQIDVKNNLQIHEDFMKK
  SPFHEHIKTDTEPKPCKGNEYGKIISDGSNQKLPLGEKPHPCGECGRGFSYSPRLPLHPNVHTGEKCFSQ
  SSHLRTHQRIHPGEKLNRCHESGDCFNKSSFHSYQSNHTGEKSYRCDSCGKGFSSSTGLIIHYRTHTGEK
  PYKCEECGKCFSQSSNFQCHQRVHTEEKPYKCEECGKGFGWSVNLRVHQRVHRGEKPYKCEECGKGFTQA
  AHFHIHQRVHTGEKPYKCDVCGKGFSHNSPLICHRRVHTGEKPYKCEACGKGFTRNTDLHIHFRVHTGEK
  PYKCKECGKGFSQASNLQVHQNVHTGEKRFKCETCGKGFSQSSKLQTHQRVHTGEKPYRCDVCGKDFSYS
  SNLKLHQVIHTGEKPYKCEECGKGFSWRSNLHAHQRVHSGEKPYKCEQCDKSFSQAIDFRVHQRVHTGEK
  PYKCGVCGKGFSQSSGLQSHQRVHTGEKPYKCDVCGKGFRYSSQFIYHQRGHTGEKPYKCEECGKGFGRS
  LNLRHHQRVHTGEKPHICEECGKAFSLPSNLRVHLGVHTREKLFKCEECGKGFSQSARLEAHQRVHTGEK
  PYKCDICDKDFRHRSRLTYHQKVHTGKKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289166
• ORF Size: 2397 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289166.1, NP_001276095.1
• RefSeq Size: 3076 bp
• RefSeq ORF: 2400 bp
• Locus ID: 7770
• UniProt ID: Q86WZ6
• Cytogenetics: 19q13.31
• Protein Families: Transcription Factors
• MW: 92.5 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]