ITGB6 (NM_001282388) Human Tagged ORF Clone

CAT#: RC239465

ITGB6 (myc-DDK-tagged) - Human integrin, beta 6 (ITGB6), transcript variant 5

Product Images

Circular map for RC239465

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITGB6
• Synonyms: AI1H
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239465 representing NM_001282388
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCACGTACAAGAATTTTACTCATCCATCTGGAGTTGGCGAAAGGTGTGATACCCCAGCAAACCTTT
  TAGCTAAAGGATGTCAATTAAACTTCATCGAAAACCCTGTCTCCCAAGTAGAAATACTTAAAAATAAGCC
  TCTCAGTGTAGGCAGACAGAAAAATAGTTCTGACATTGTTCAGATTGCGCCTCAAAGCTTGATCCTTAAG
  TTGAGACCAGGTGGTGCGCAGACTCTGCAGGTGCATGTCCGCCAGACTGAGGACTACCCGGTGGATTTGT
  ATTACCTCATGGACCTCTCCGCCTCCATGGATGACGACCTCAACACAATAAAGGAGCTGGGCTCCCGGCT
  TTCCAAAGAGATGTCTAAATTAACCAGCAACTTTAGACTGGGCTTCGGATCTTTTGTGGAAAAACCTGTA
  TCCCCTTTTGTGAAAACAACACCAGAAGAAATTGCCAACCCTTGCAGTAGTATTCCATACTTCTGTTTAC
  CTACATTTGGATTCAAGCACATTTTGCCATTGACAAATGATGCTGAAAGATTCAATGAAATTGTGAAGAA
  TCAGAAAATTTCTGCTAATATTGACACACCCGAAGGTGGATTTGATGCAATTATGCAAGCTGCTGTGTGT
  AAGGAAAAAATTGGCTGGCGGAATGACTCCCTCCACCTCCTGGTCTTTGTGAGTGATGCTGATTCTCATT
  TTGGAATGGACAGCAAACTAGCAGGCATCGTCATTCCTAATGACGGGCTCTGTCACTTGGACAGCAAGAA
  TGAATACTCCATGTCAACTGTCTTGGAATATCCAACAATTGGACAACTCATTGATAAACTGGTACAAAAC
  AACGTGTTATTGATCTTCGCTGTAACCCAAGAACAAGTTCATTTATATGAGAATTACGCAAAACTTATTC
  CTGGAGCTACAGTAGGTCTACTTCAGAAGGACTCCGGAAACATTCTCCAGCTGATCATCTCAGCTTATGA
  AGAACTGCGGTCTGAGGTGGAACTGGAAGTATTAGGAGACACTGAAGGACTCAACTTGTCATTTACAGCC
  ATCTGTAACAACGGTACCCTCTTCCAACACCAAAAGAAATGCTCTCACATGAAAGTGGGAGACACAGCTT
  CCTTCAGCGTGACTGTGAATATCCCACACTGCGAGAGAAGAAGCAGGCACATTATCATAAAGCCTGTGGG
  GCTGGGGGATGCCCTGGAATTACTTGTCAGCCCAGAATGCAACTGCGACTGTCAGAAAGAAGTGGAAGTG
  AACAGCTCCAAATGTCACCACGGGAACGGCTCTTTCCAGTGTGGGGTGTGTGCCTGCCACCCTGGCCACA
  TGGGGCCTCGCTGTGAGTGTGGCGAGGACATGCTGAGCACAGATTCCTGCAAGGAGGCCCCAGATCATCC
  CTCCTGCAGCGGAAGGGGTGACTGCTACTGTGGGCAGTGTATCTGCCACTTGTCTCCCTATGGAAACATT
  TATGGGCCTTATTGCCAGTGTGACAATTTCTCCTGCGTGAGACACAAAGGGCTGCTCTGCGGAGGTAACG
  GCGACTGTGACTGTGGTGAATGTGTGTGCAGGAGCGGCTGGACTGGCGAGTACTGCAACTGCACCACCAG
  CACGGACTCCTGCGTCTCTGAAGATGGAGTGCTCTGCAGCGGGCGCGGGGACTGTGTTTGTGGCAAGTGT
  GTTTGCACAAACCCTGGAGCCTCAGGACCAACCTGTGAACGATGTCCTACCTGTGGTGACCCCTGTAACT
  CTAAACGGAGCTGCATTGAGTGCCACCTGTCAGCAGCTGGCCAAGCCCGAGAAGAATGTGTGGACAAGTG
  CAAACTAGCTGGTGCGACCATCAGTGAAGAAGAAGATTTCTCAAAGGATGGTTCTGTTTCCTGCTCTCTG
  CAAGGAGAAAATGAATGTCTTATTACATTCCTAATAACTACAGATAATGAGGGGAAAACCATCATTCACA
  GCATCAATGAAAAAGATTGTCCGAAGCCTCCAAACATTCCCATGATCATGTTAGGGGTTTCCCTGGCTAT
  TCTTCTCATCGGGGTTGTCCTACTGTGCATCTGGAAGCTACTGGTGTCATTTCATGATCGTAAAGAAGTT
  GCCAAATTTGAAGCAGAACGATCAAAAGCCAAGTGGCAAACGGGAACCAATCCACTCTACAGAGGATCCA
  CAAGTACTTTTAAAAATGTAACTTATAAACACAGGGAAAAACAAAAGGTAGACCTTTCCACAGATTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239465 representing NM_001282388
  Red=Cloning site Green=Tags(s)
  
  MITYKNFTHPSGVGERCDTPANLLAKGCQLNFIENPVSQVEILKNKPLSVGRQKNSSDIVQIAPQSLILK
  LRPGGAQTLQVHVRQTEDYPVDLYYLMDLSASMDDDLNTIKELGSRLSKEMSKLTSNFRLGFGSFVEKPV
  SPFVKTTPEEIANPCSSIPYFCLPTFGFKHILPLTNDAERFNEIVKNQKISANIDTPEGGFDAIMQAAVC
  KEKIGWRNDSLHLLVFVSDADSHFGMDSKLAGIVIPNDGLCHLDSKNEYSMSTVLEYPTIGQLIDKLVQN
  NVLLIFAVTQEQVHLYENYAKLIPGATVGLLQKDSGNILQLIISAYEELRSEVELEVLGDTEGLNLSFTA
  ICNNGTLFQHQKKCSHMKVGDTASFSVTVNIPHCERRSRHIIIKPVGLGDALELLVSPECNCDCQKEVEV
  NSSKCHHGNGSFQCGVCACHPGHMGPRCECGEDMLSTDSCKEAPDHPSCSGRGDCYCGQCICHLSPYGNI
  YGPYCQCDNFSCVRHKGLLCGGNGDCDCGECVCRSGWTGEYCNCTTSTDSCVSEDGVLCSGRGDCVCGKC
  VCTNPGASGPTCERCPTCGDPCNSKRSCIECHLSAAGQAREECVDKCKLAGATISEEEDFSKDGSVSCSL
  QGENECLITFLITTDNEGKTIIHSINEKDCPKPPNIPMIMLGVSLAILLIGVVLLCIWKLLVSFHDRKEV
  AKFEAERSKAKWQTGTNPLYRGSTSTFKNVTYKHREKQKVDLSTDC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282388
• ORF Size: 2238 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282388.2
• RefSeq Size: 4613 bp
• RefSeq ORF: 2241 bp
• Locus ID: 3694
• UniProt ID: P18564
• Cytogenetics: 2q24.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
• MW: 82 kDa
• Gene Summary: This gene encodes a protein that is a member of the integrin superfamily. Members of this family are adhesion receptors that function in signaling from the extracellular matrix to the cell. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The encoded protein forms a dimer with an alpha v chain and this heterodimer can bind to ligands like fibronectin and transforming growth factor beta 1. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]