ITGB6 (NM_001282354) Human Tagged ORF Clone

CAT#: RC239325

ITGB6 (myc-DDK-tagged) - Human integrin, beta 6 (ITGB6), transcript variant 3

Product Images

Circular map for RC239325

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITGB6
• Synonyms: AI1H
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239325 representing NM_001282354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGATTGAACTGCTTTGCCTGTTCTTTCTATTTCTAGGAAGGAATGATCACGTACAAGGTGGTGCGC
  AGACTCTGCAGGTGCATGTCCGCCAGACTGAGGACTACCCGGTGGATTTGTATTACCTCATGGACCTCTC
  CGCCTCCATGGATGACGACCTCAACACAATAAAGGAGCTGGGCTCCCGGCTTTCCAAAGAGATGTCTAAA
  TTAACCAGCAACTTTAGACTGGGCTTCGGATCTTTTGTGGAAAAACCTGTATCCCCTTTTGTGAAAACAA
  CACCAGAAGAAATTGCCAACCCTTGCAGTAGTATTCCATACTTCTGTTTACCTACATTTGGATTCAAGCA
  CATTTTGCCATTGACAAATGATGCTGAAAGATTCAATGAAATTGTGAAGAATCAGAAAATTTCTGCTAAT
  ATTGACACACCCGAAGGTGGATTTGATGCAATTATGCAAGCTGCTGTGTGTAAGGAAAAAATTGGCTGGC
  GGAATGACTCCCTCCACCTCCTGGTCTTTGTGAGTGATGCTGATTCTCATTTTGGAATGGACAGCAAACT
  AGCAGGCATCGTCATTCCTAATGACGGGCTCTGTCACTTGGACAGCAAGAATGAATACTCCATGTCAACT
  GTCTTGGAATATCCAACAATTGGACAACTCATTGATAAACTGGTACAAAACAACGTGTTATTGATCTTCG
  CTGTAACCCAAGAACAAGTTCATTTATATGAGAATTACGCAAAACTTATTCCTGGAGCTACAGTAGGTCT
  ACTTCAGAAGGACTCCGGAAACATTCTCCAGCTGATCATCTCAGCTTATGAAGAACTGCGGTCTGAGGTG
  GAACTGGAAGTATTAGGAGACACTGAAGGACTCAACTTGTCATTTACAGCCATCTGTAACAACGGTACCC
  TCTTCCAACACCAAAAGAAATGCTCTCACATGAAAGTGGGAGACACAGCTTCCTTCAGCGTGACTGTGAA
  TATCCCACACTGCGAGAGAAGAAGCAGGCACATTATCATAAAGCCTGTGGGGCTGGGGGATGCCCTGGAA
  TTACTTGTCAGCCCAGAATGCAACTGCGACTGTCAGAAAGAAGTGGAAGTGAACAGCTCCAAATGTCACC
  ACGGGAACGGCTCTTTCCAGTGTGGGGTGTGTGCCTGCCACCCTGGCCACATGGGGCCTCGCTGTGAGTG
  TGGCGAGGACATGCTGAGCACAGATTCCTGCAAGGAGGCCCCAGATCATCCCTCCTGCAGCGGAAGGGGT
  GACTGCTACTGTGGGCAGTGTATCTGCCACTTGTCTCCCTATGGAAACATTTATGGGCCTTATTGCCAGT
  GTGACAATTTCTCCTGCGTGAGACACAAAGGGCTGCTCTGCGGAGGTAACGGCGACTGTGACTGTGGTGA
  ATGTGTGTGCAGGAGCGGCTGGACTGGCGAGTACTGCAACTGCACCACCAGCACGGACTCCTGCGTCTCT
  GAAGATGGAGTGCTCTGCAGCGGGCGCGGGGACTGTGTTTGTGGCAAGTGTGTTTGCACAAACCCTGGAG
  CCTCAGGACCAACCTGTGAACGATGTCCTACCTGTGGTGACCCCTGTAACTCTAAACGGAGCTGCATTGA
  GTGCCACCTGTCAGCAGCTGGCCAAGCCCGAGAAGAATGTGTGGACAAGTGCAAACTAGCTGGTGCGACC
  ATCAGTGAAGAAGAAGATTTCTCAAAGGATGGTTCTGTTTCCTGCTCTCTGCAAGGAGAAAATGAATGTC
  TTATTACATTCCTAATAACTACAGATAATGAGGGGAAAACCATCATTCACAGCATCAATGAAAAAGATTG
  TCCGAAGCCTCCAAACATTCCCATGATCATGTTAGGGGTTTCCCTGGCTATTCTTCTCATCGGGGTTGTC
  CTACTGTGCATCTGGAAGCTACTGGTGTCATTTCATGATCGTAAAGAAGTTGCCAAATTTGAAGCAGAAC
  GATCAAAAGCCAAGTGGCAAACGGGAACCAATCCACTCTACAGAGGATCCACAAGTACTTTTAAAAATGT
  AACTTATAAACACAGGGAAAAACAAAAGGTAGACCTTTCCACAGATTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239325 representing NM_001282354
  Red=Cloning site Green=Tags(s)
  
  MGIELLCLFFLFLGRNDHVQGGAQTLQVHVRQTEDYPVDLYYLMDLSASMDDDLNTIKELGSRLSKEMSK
  LTSNFRLGFGSFVEKPVSPFVKTTPEEIANPCSSIPYFCLPTFGFKHILPLTNDAERFNEIVKNQKISAN
  IDTPEGGFDAIMQAAVCKEKIGWRNDSLHLLVFVSDADSHFGMDSKLAGIVIPNDGLCHLDSKNEYSMST
  VLEYPTIGQLIDKLVQNNVLLIFAVTQEQVHLYENYAKLIPGATVGLLQKDSGNILQLIISAYEELRSEV
  ELEVLGDTEGLNLSFTAICNNGTLFQHQKKCSHMKVGDTASFSVTVNIPHCERRSRHIIIKPVGLGDALE
  LLVSPECNCDCQKEVEVNSSKCHHGNGSFQCGVCACHPGHMGPRCECGEDMLSTDSCKEAPDHPSCSGRG
  DCYCGQCICHLSPYGNIYGPYCQCDNFSCVRHKGLLCGGNGDCDCGECVCRSGWTGEYCNCTTSTDSCVS
  EDGVLCSGRGDCVCGKCVCTNPGASGPTCERCPTCGDPCNSKRSCIECHLSAAGQAREECVDKCKLAGAT
  ISEEEDFSKDGSVSCSLQGENECLITFLITTDNEGKTIIHSINEKDCPKPPNIPMIMLGVSLAILLIGVV
  LLCIWKLLVSFHDRKEVAKFEAERSKAKWQTGTNPLYRGSTSTFKNVTYKHREKQKVDLSTDC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282354
• ORF Size: 2079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282354.1, NP_001269283.1
• RefSeq Size: 4408 bp
• RefSeq ORF: 2082 bp
• Locus ID: 3694
• Cytogenetics: 2q24.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
• MW: 76.3 kDa
• Gene Summary: This gene encodes a protein that is a member of the integrin superfamily. Members of this family are adhesion receptors that function in signaling from the extracellular matrix to the cell. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The encoded protein forms a dimer with an alpha v chain and this heterodimer can bind to ligands like fibronectin and transforming growth factor beta 1. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]