PCDH1 (NM_001278615) Human Tagged ORF Clone

CAT#: RC239296

PCDH1 (myc-DDK-tagged) - Human protocadherin 1 (PCDH1), transcript variant 4

Product Images

Circular map for RC239296

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDH1
• Synonyms: PC42; PCDH42
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239296 representing NM_001278615
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGACAATGCCCCCACCATTGAGATCCGGGGCATAGGGCTAGTGACTCATCAAGATGGGATGGCTA
  ACATCTCAGAGGATGTGGCAGAGGAGACAGCTGTGGCCCTGGTGCAGGTGTCTGACCGAGATGAGGGAGA
  GAATGCAGCTGTCACCTGTGTGGTGGCAGGTGATGTGCCCTTCCAGCTGCGCCAGGCCAGTGAGACAGGC
  AGTGACAGCAAGAAGAAGTATTTCCTGCAGACTACCACCCCGCTAGACTACGAGAAGGTCAAAGACTACA
  CCATTGAGATTGTGGCTGTGGACTCTGGCAACCCCCCACTCTCCAGCACTAACTCCCTCAAGGTGCAGGT
  GGTGGACGTCAATGACAACGCACCTGTCTTCACTCAGAGTGTCACTGAGGTCGCCTTCCCGGAAAACAAC
  AAGCCTGGTGAAGTGATTGCTGAGATCACTGCCAGTGATGCTGACTCTGGCTCTAATGCTGAGCTGGTTT
  ACTCTCTGGAGCCTGAGCCGGCTGCTAAGGGCCTCTTCACCATCTCACCCGAGACTGGAGAGATCCAGGT
  GAAGACATCTCTGGATCGGGAACAGCGGGAGAGCTATGAGTTGAAGGTGGTGGCAGCTGACCGGGGCAGT
  CCTAGCCTCCAGGGCACAGCCACTGTCCTTGTCAATGTGCTGGACTGCAATGACAATGACCCCAAATTTA
  TGCTGAGTGGCTACAACTTCTCAGTGATGGAGAACATGCCAGCACTGAGTCCAGTGGGCATGGTGACTGT
  CATTGATGGAGACAAGGGGGAGAATGCCCAGGTGCAGCTCTCAGTGGAGCAGGACAACGGTGACTTTGTT
  ATCCAGAATGGCACAGGCACCATCCTATCCAGCCTGAGCTTTGATCGAGAGCAACAAAGCACCTACACCT
  TCCAGCTGAAGGCAGTGGATGGTGGCGTCCCACCTCGCTCAGCTTACGTTGGTGTCACCATCAATGTGCT
  GGACGAGAATGACAACGCACCCTATATCACTGCCCCTTCTAACACCTCTCACAAGCTGCTGACCCCCCAG
  ACACGTCTTGGTGAGACGGTCAGCCAGGTGGCAGCCGAGGACTTTGACTCTGGTGTCAATGCTGAGCTGA
  TCTACAGCATTGCAGGTGGCAACCCTTATGGACTCTTCCAGATTGGGTCACATTCAGGTGCCATCACCCT
  GGAGAAGGAGATTGAGCGGCGCCACCATGGGCTACACCGCCTGGTGGTGAAGGTCAGTGACCGCGGCAAG
  CCCCCACGCTATGGCACAGCCTTGGTCCATCTTTATGTCAATGAGACTCTGGCCAACCGCACGCTGCTGG
  AGACCCTCCTGGGCCACAGCCTGGACACGCCGCTGGATATTGACATTGCTGGGGATCCAGAATATGAGCG
  CTCCAAGCAGCGTGGCAACATTCTCTTTGGTGTGGTGGCTGGTGTGGTGGCCGTGGCCTTGCTCATCGCC
  CTGGCGGTTCTTGTGCGCTACTGCAGACAGCGGGAGGCCAAAAGTGGTTACCAGGCTGGTAAGAAGGAGA
  CCAAGGACCTGTATGCCCCCAAGCCCAGTGGCAAGGCCTCCAAGGGAAACAAAAGCAAAGGCAAGAAGAG
  CAAGTCCCCAAAGCCCGTGAAGCCAGTGGAGGACGAGGATGAGGCCGGGCTGCAGAAGTCCCTCAAGTTC
  AACCTGATGAGCGATGCCCCTGGGGACAGTCCCCGCATCCACCTGCCCCTCAACTACCCACCAGGCAGCC
  CTGACCTGGGCCGCCACTATCGCTCTAACTCCCCACTGCCTTCCATCCAGCTGCAGCCCCAGTCACCCTC
  AGCCTCCAAGAAGCACCAGGTGGTACAGGACCTGCCACCTGCAAACACATTCGTGGGCACCGGGGACACC
  ACGTCCACGGGCTCTGAGCAGTACTCCGACTACAGCTACCGCACCAACCCCCCCAAATACCCCAGCAAGC
  AGGTAGGCCAGCCCTTTCAGCTCAGCACACCCCAGCCCCTACCCCACCCCTACCACGGAGCCATCTGGAC
  CGAGGTGTGGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239296 representing NM_001278615
  Red=Cloning site Green=Tags(s)
  
  MNDNAPTIEIRGIGLVTHQDGMANISEDVAEETAVALVQVSDRDEGENAAVTCVVAGDVPFQLRQASETG
  SDSKKKYFLQTTTPLDYEKVKDYTIEIVAVDSGNPPLSSTNSLKVQVVDVNDNAPVFTQSVTEVAFPENN
  KPGEVIAEITASDADSGSNAELVYSLEPEPAAKGLFTISPETGEIQVKTSLDREQRESYELKVVAADRGS
  PSLQGTATVLVNVLDCNDNDPKFMLSGYNFSVMENMPALSPVGMVTVIDGDKGENAQVQLSVEQDNGDFV
  IQNGTGTILSSLSFDREQQSTYTFQLKAVDGGVPPRSAYVGVTINVLDENDNAPYITAPSNTSHKLLTPQ
  TRLGETVSQVAAEDFDSGVNAELIYSIAGGNPYGLFQIGSHSGAITLEKEIERRHHGLHRLVVKVSDRGK
  PPRYGTALVHLYVNETLANRTLLETLLGHSLDTPLDIDIAGDPEYERSKQRGNILFGVVAGVVAVALLIA
  LAVLVRYCRQREAKSGYQAGKKETKDLYAPKPSGKASKGNKSKGKKSKSPKPVKPVEDEDEAGLQKSLKF
  NLMSDAPGDSPRIHLPLNYPPGSPDLGRHYRSNSPLPSIQLQPQSPSASKKHQVVQDLPPANTFVGTGDT
  TSTGSEQYSDYSYRTNPPKYPSKQVGQPFQLSTPQPLPHPYHGAIWTEVWE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278615
• ORF Size: 2043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278615.1, NP_001265544.1
• RefSeq Size: 2925 bp
• RefSeq ORF: 2046 bp
• Locus ID: 5097
• UniProt ID: Q08174
• Cytogenetics: 5q31.3
• Protein Families: Transcription Factors, Transmembrane
• MW: 74 kDa
• Gene Summary: This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene. [provided by RefSeq, Jul 2008]