MCCC1 (NM_001293273) Human Tagged ORF Clone

CAT#: RC239070

MCCC1 (myc-DDK-tagged) - Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), transcript variant 2

Product Images

Circular map for RC239070

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MCCC1
• Synonyms: MCC-B; MCCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239070 representing NM_001293273
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGTGGAGGCAAAGAACCATGAAGTACACAACAGCCACAGGCAGATGAAGCATATTCCATCGGCCC
  CGCTCCCTCCCAGCAGAGCTACCTATCTATGGAGAAAATCATTCAAGTGGCCAAGACCTCTGCTGCACAG
  CACATCCAAATCCATAATGGCTGCTGCTGGAGTACCTGTTGTGGAGGGTTATCATGGTGAGGACCAATCA
  GACCAGTGCCTGAAGGAACACGCCAGGAGAATTGGCTATCCTGTCATGATTAAAGCCGTCCGGGGTGGAG
  GAGGAAAAGGAATGAGGATTGTTAGATCAGAACAAGAATTTCAAGAACAGTTAGAGTCAGCACGGAGAGA
  AGCTAAGAAGTCTTTCAATGATGATGCTATGCTGATCGAGAAGTTTGTAGACACACCGAGGCATGTAGAA
  GTCCAGGTGTTTGGTGATCACCATGGCAATGCTGTGTACTTGTTTGAAAGAGACTGTAGTGTGCAGAGGC
  GACATCAGAAGATCATTGAGGAGGCCCCAGCGCCTGGTATTAAATCTGAAGTAAGAAAAAAGCTGGGAGA
  AGCTGCAGTCAGAGCTGCTAAAGCTGTAAATTATGTTGGAGCAGGGACTGTGGAGTTTATTATGGACTCA
  AAACATAATTTCTGTTTCATGGAGATGAATACAAGGCTGCAAGTGGAACATCCTGTTACTGAGATGATCA
  CAGGAACTGACTTGGTGGAGTGGCAGCTTAGAATTGCAGCAGGAGAGAAGATTCCTTTGAGCCAGGAAGA
  AATAACTCTGCAGGGCCATGCCTTCGAAGCTAGAATATATGCAGAAGATCCTAGCAATAACTTCATGCCT
  GTGGCAGGCCCATTAGTGCACCTCTCTACTCCTCGAGCAGACCCTTCCACCAGGATTGAAACTGGAGTAC
  GGCAAGGAGACGAAGTTTCCGTGCATTATGACCCCATGATTGCGAAGCTGGTCGTGTGGGCAGCAGATCG
  CCAGGCGGCATTGACAAAACTGAGGTACAGCCTTCGTCAGTACAATATTGTTGGACTGCACACCAACATT
  GACTTCTTACTCAACCTGTCTGGCCACCCAGAGTTTGAAGCTGGGAACGTGCACACTGATTTCATCCCTC
  AACACCACAAACAGTTGTTGCTCAGTCGGAAGGCTGCAGCCAAAGAGTCTTTATGCCAGGCAGCCCTGGG
  TCTCATCCTCAAGGAGAAAGCCATGACCGACACTTTCACTCTTCAGGCACATGATCAATTCTCTCCATTT
  TCGTCTAGCAGTGGAAGAAGACTGAATATCTCGTATACCAGAAACATGACTCTTAAAGATGGTAAAAACA
  ATGTAGCCATAGCTGTAACGTATAACCATGATGGGTCTTATAGCATGCAGATTGAAGATAAAACTTTCCA
  AGTCCTTGGTAATCTTTACAGCGAGGGAGACTGCACTTACCTGAAATGTTCTGTTAATGGAGTTGCTAGT
  AAAGCGAAGCTGATTATCCTGGAAAACACTATTTACCTATTTTCCAAGGAAGGAAGTATTGAGATTGACA
  TTCCAGTCCCCAAATACTTATCTTCTGTGAGCTCACAAGAAACTCAGGGCGGCCCCTTAGCTCCTATGAC
  TGGAACCATTGAAAAGGTGTTTGTCAAAGCTGGAGACAAAGTGAAAGCGGGAGATTCCCTCATGGTTATG
  ATCGCCATGAAGATGGAGCATACCATAAAGTCTCCAAAGGATGGCACAGTAAAGAAAGTGTTCTACAGAG
  AAGGTGCTCAGGCCAACAGACACACTCCTTTAGTCGAGTTTGAGGAGGAAGAATCAGACAAAAGGGAATC
  GGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239070 representing NM_001293273
  Red=Cloning site Green=Tags(s)
  
  MGVEAKNHEVHNSHRQMKHIPSAPLPPSRATYLWRKSFKWPRPLLHSTSKSIMAAAGVPVVEGYHGEDQS
  DQCLKEHARRIGYPVMIKAVRGGGGKGMRIVRSEQEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVE
  VQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPAPGIKSEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDS
  KHNFCFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLQGHAFEARIYAEDPSNNFMP
  VAGPLVHLSTPRADPSTRIETGVRQGDEVSVHYDPMIAKLVVWAADRQAALTKLRYSLRQYNIVGLHTNI
  DFLLNLSGHPEFEAGNVHTDFIPQHHKQLLLSRKAAAKESLCQAALGLILKEKAMTDTFTLQAHDQFSPF
  SSSSGRRLNISYTRNMTLKDGKNNVAIAVTYNHDGSYSMQIEDKTFQVLGNLYSEGDCTYLKCSVNGVAS
  KAKLIILENTIYLFSKEGSIEIDIPVPKYLSSVSSQETQGGPLAPMTGTIEKVFVKAGDKVKAGDSLMVM
  IAMKMEHTIKSPKDGTVKKVFYREGAQANRHTPLVEFEEEESDKRESE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293273
• ORF Size: 1824 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293273.1, NP_001280202.1
• RefSeq Size: 2302 bp
• RefSeq ORF: 1827 bp
• Locus ID: 56922
• UniProt ID: Q96RQ3
• Cytogenetics: 3q27.1
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• MW: 68.4 kDa
• Gene Summary: This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. [provided by RefSeq, Jul 2008]