HNRPM (HNRNPM) (NM_001297418) Human Tagged ORF Clone

CAT#: RC239026

HNRNPM (myc-DDK-tagged) - Human heterogeneous nuclear ribonucleoprotein M (HNRNPM), transcript variant 3

Product Images

Circular map for RC239026

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HNRPM
• Synonyms: CEAR; hnRNP M; HNRNPM4; HNRPM; HNRPM4; HTGR1; NAGR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239026 representing NM_001297418
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGAGCATGAAAAAAGCTGCGGAAGTCCTAAACAAGCATAGTCTGAGCGGAAGACCACTGAAAG
  TCAAAGAAGATCCTGATGGTGAACATGCCAGGAGAGCAATGCAAAAGGTGATGGCTACGACTGGTGGGAT
  GGGTATGGGACCAGGTGGCCCAGGAATGATTACTATCCCACCCAGTATCCTAAATAATCCCAACATCCCA
  AATGAGATTATCCATGCATTACAGGCTGGAAGACTTGGAAGCACAGTATTTGTAGCAAATCTGGATTATA
  AAGTTGGCTGGAAGAAACTGAAGGAAGTATTTAGTATGGCTGGTGTGGTGGTCCGAGCAGACATTCTTGA
  AGATAAAGATGGAAAAAGTCGTGGAATAGGCACTGTTACTTTTGAACAGTCCATTGAAGCTGTGCAAGCT
  ATATCTATGTTCAATGGCCAGCTGCTATTTGATAGACCAATGCACGTCAAGATGGATGAGAGGGCCTTAC
  CAAAAGGAGATTTCTTCCCTCCTGAGCGTCCACAACAACTTCCCCATGGCCTTGGTGGTATTGGCATGGG
  GTTAGGACCAGGAGGGCAACCCATTGATGCCAATCACCTGAATAAAGGCATCGGAATGGGAAACATAGGT
  CCCGCAGGAATGGAGGGGCCCTTTGGTGGTGGTATGGAAAACATGGGTCGATTTGGATCTGGGATGAACA
  TGGGCAGGATAAATGAAATCCTAAGTAATGCACTGAAGAGAGGAGAGATCATTGCAAAGCAGGGAGGAGG
  TGGAGGTGGAGGAAGCGTCCCTGGGATCGAGAGGATGGGTCCTGGCATTGACCGCCTCGGGGGTGCCGGC
  ATGGAGCGCATGGGCGCGGGCCTGGGCCACGGCATGGATCGCGTGGGCTCCGAGATCGAGCGCATGGGCC
  TGGTCATGGACCGCATGGGCTCCGTGGAGCGCATGGGCTCCGGCATTGAGCGCATGGGCCCGCTGGGCCT
  CGACCACATGGCCTCCAGCATTGAGCGCATGGGCCAGACCATGGAGCGCATTGGCTCTGGCGTGGAGCGC
  ATGGGTGCCGGCATGGGCTTCGGCCTTGAGCGCATGGCCGCTCCCATCGACCGTGTGGGCCAGACCATTG
  AGCGCATGGGCTCTGGCGTGGAGCGCATGGGCCCTGCCATCGAGCGCATGGGCCTGAGCATGGAGCGCAT
  GGTGCCCGCAGGTATGGGAGCTGGCCTGGAGCGCATGGGCCCCGTGATGGATCGCATGGCCACCGGCCTG
  GAGCGCATGGGCGCCAACAATCTGGAGCGGATGGGCCTGGAGCGCATGGGCGCCAACAGCCTCGAGCGCA
  TGGGCCTGGAGCGCATGGGTGCCAACAGCCTCGAGCGCATGGGCCCTGCCATGGGCCCGGCCCTGGGCGC
  TGGCATTGAGCGCATGGGCCTGGCCATGGGTGGCGGTGGCGGTGCCAGCTTTGACCGTGCCATCGAGATG
  GAGCGTGGCAACTTCGGAGGAAGCTTCGCAGGTTCCTTTGGTGGAGCTGGAGGCCATGCTCCTGGGGTGG
  CCAGGAAGGCCTGCCAGATATTTGTGAGAAATCTGCCATTCGATTTCACATGGAAGATGCTAAAGGACAA
  ATTCAACGAGTGCGGCCACGTGCTGTACGCCGACATCAAGATGGAGAATGGGAAGTCCAAGGGGTGTGGC
  GTGGTTAAGTTCGAGTCGCCAGAGGTGGCCGAGAGAGCCTGCCGGATGATGAATGGCATGAAGCTGAGTG
  GCCGAGAGATTGACGTTCGAATTGATAGAAACGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239026 representing NM_001297418
  Red=Cloning site Green=Tags(s)
  
  MEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIP
  NEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQA
  ISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLNKGIGMGNIG
  PAGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAG
  MERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSGIERMGPLGLDHMASSIERMGQTMERIGSGVER
  MGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRMATGL
  ERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGGGGASFDRAIEM
  ERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCG
  VVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001297418
• ORF Size: 1785 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001297418.2
• RefSeq Size: 2540 bp
• RefSeq ORF: 1788 bp
• Locus ID: 4670
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome
• MW: 63.4 kDa
• Gene Summary: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]