DRP1 (DNM1L) (NM_001278466) Human Tagged ORF Clone

CAT#: RC238754

DNM1L (myc-DDK-tagged) - Human dynamin 1-like (DNM1L), transcript variant 7

Product Images

Circular map for RC238754

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DRP1
• Synonyms: DLP1; DRP1; DVLP; DYMPLE; EMPF; EMPF1; HDYNIV; OPA5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238754 representing NM_001278466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCACAAGAAGATAAACGGAAAACAACAGGAGAAGAAAATGACCCTGCTACATGGAAAAACTCAAG
  ACACCTTTCTAAAGGGGTGGAAGCAGAAGAATGGGGTAAATTTCTTCACACCAAAAATAAGGAGCCAGCT
  AGATATTAACAACAAGAAGAGTGTAACTGATTCAATCCGTGATGAGTATGCTTTTCTTCAAAAGAAATAT
  CCATCTCTGGCCAATAGAAATGGAACAAAGTATCTTGCTAGGACTCTAAACAGGTTACTGATGCATCACA
  TCAGAGATTGTTTACCAGAGTTGAAAACAAGAATAAATGTTCTAGCTGCTCAGTATCAGTCTCTTCTAAA
  TAGCTACGGTGAACCCGTGGATGATAAAAGTGCTACTTTACTCCAACTTATTACCAAATTTGCCACAGAA
  TATTGTAACACTATTGAAGGAACTGCAAAATATATTGAAACTTCGGAGCTATGCGGTGGTGCTAGAATTT
  GTTATATTTTCCATGAGACTTTTGGGCGAACCTTAGAATCTGTTGATCCACTTGGTGGCCTTAACACTAT
  TGACATTTTGACTGCCATTAGAAATGCTACTGGTCCTCGTCCTGCTTTATTTGTGCCTGAGGTTTCATTT
  GAGTTACTGGTGAAGCGGCAAATCAAACGTCTAGAAGAGCCCAGCCTCCGCTGTGTGGAACTGGTTCATG
  AGGAAATGCAAAGGATCATTCAGCACTGTAGCAATTACAGTACACAGGAATTGTTACGATTTCCTAAACT
  TCATGATGCCATAGTTGAAGTGGTGACTTGTCTTCTTCGTAAAAGGTTGCCTGTTACAAATGAAATGGTC
  CATAACTTAGTGGCAATTGAACTGGCTTATATCAACACAAAACATCCAGACTTTGCTGATGCTTGTGGGC
  TAATGAACAATAATATAGAGGAACAAAGGAGAAACAGGCTAGCCAGAGAATTACCTTCAGCTGTATCACG
  AGACAAGTCTTCTAAAGTTCCAAGTGCTTTGGCACCTGCCTCCCAGGAGCCCTCCCCCGCTGCTTCTGCT
  GAGGCTGATGGCAAGTTAATTCAGGACAGCAGAAGAGAAACTAAAAATGTTGCATCTGGAGGTGGTGGGG
  TTGGAGATGGTGTTCAAGAACCAACCACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAAGCTGAAGA
  GTTATTAGCAGAAGAAAAATCAAAACCCATTCCAATTATGCCAGCCAGTCCACAAAAAGGTCATGCCGTG
  AACCTGCTAGATGTGCCAGTTCCTGTTGCACGAAAACTATCTGCTCGGGAACAGCGAGATTGTGAGGTTA
  TTGAACGACTCATTAAATCATATTTTCTCATTGTCAGAAAGAATATTCAAGACAGTGTGCCAAAGGCAGT
  AATGCATTTTTTGGTTAATCATGTGAAAGACACTCTTCAGAGTGAGCTAGTAGGCCAGCTGTATAAATCA
  TCCTTATTGGATGATCTTCTGACAGAATCTGAGGACATGGCACAGCGCAGGAAAGAAGCAGCTGATATGC
  TAAAGGCATTACAAGGAGCCAGTCAAATTATTGCTGAAATCCGGGAGACTCATCTTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238754 representing NM_001278466
  Red=Cloning site Green=Tags(s)
  
  MFHKKINGKQQEKKMTLLHGKTQDTFLKGWKQKNGVNFFTPKIRSQLDINNKKSVTDSIRDEYAFLQKKY
  PSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATE
  YCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSF
  ELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMV
  HNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKSSKVPSALAPASQEPSPAASA
  EADGKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAV
  NLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKS
  SLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278466
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278466.1, NP_001265395.1
• RefSeq Size: 4211 bp
• RefSeq ORF: 1602 bp
• Locus ID: 10059
• UniProt ID: O00429
• Cytogenetics: 12p11.21
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• MW: 60.5 kDa
• Gene Summary: This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]