GLS2 (NM_001280796) Human Tagged ORF Clone

CAT#: RC237458

GLS2 (myc-DDK-tagged) - Human glutaminase 2 (liver, mitochondrial) (GLS2), transcript variant 3

Product Images

Circular map for RC237458

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GLS2
• Synonyms: GA; GLS; hLGA; LGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237458 representing NM_001280796
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATACATGGGTTTCAGCAATGCCACGGAAGGTAATTTTTCCCCTTCTCCCAACACCCACTCCTCCAA
  CTTCAAGTACTAAATTGGGTGTTTGCTTCAGATTCCAGTCAGAGAAGGAAACAGGGGATCGGAATTATGC
  CATCGGCTATTATCTCAAGGAAAAGAAGTGCTTTCCTAAGGGGGTGGACATGATGGCTGCCCTTGATCTC
  TACTTCCAGCTGTGTTCTGTGGAGGTCACTTGTGAATCAGGCAGTGTCATGGCAGCCACCCTCGCCAACG
  GTGGGATCTGCCCCATCACAGGCGAGAGTGTGCTGAGTGCTGAAGCAGTGCGCAACACCCTCAGCCTCAT
  GCATTCCTGCGGCATGTATGACTTCTCTGGCCAGTTTGCCTTCCACGTGGGCCTGCCAGCCAAGTCAGCT
  GTATCAGGAGCCATCCTCCTGGTGGTACCCAATGTCATGGGAATGATGTGCCTGTCACCCCCATTGGACA
  AGCTGGGGAACAGCCATAGGGGGACCAGCTTCTGCCAGAAGTTGGTGTCTCTCTTCAATTTCCACAACTA
  TGACAACCTGAGGCACTGTGCTCGGAAGTTAGACCCACGGCGTGAAGGGGCAGAAATTCGGAACAAGACT
  GTGGTCAACCTGTTATTTGCTGCCTATAGTGGCGATGTCTCAGCTCTTCGAAGGTTTGCCTTGTCAGCCA
  TGGATATGGAACAGAAAGACTATGACTCGCGCACAGCTCTGCATGTTGCTGCAGCTGAAGGACACATCGA
  AGTTGTTAAATTCCTGATCGAGGCTTGCAAAGTGAATCCTTTTGCCAAGGACAGGTGGGGCAACATTCCC
  CTGGATGATGCTGTGCAGTTCAACCATCTGGAGGTGGTCAAACTGCTTCAAGATTACCAGGACTCCTACA
  CACTCTCTGAAACTCAGGCTGAGGCAGCAGCTGAGGCCCTGTCCAAAGAGAACTTAGAAAGCATGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237458 representing NM_001280796
  Red=Cloning site Green=Tags(s)
  
  MNTWVSAMPRKVIFPLLPTPTPPTSSTKLGVCFRFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDL
  YFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSA
  VSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKT
  VVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIP
  LDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001280796
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001280796.2
• RefSeq Size: 2721 bp
• RefSeq ORF: 981 bp
• Locus ID: 27165
• Cytogenetics: 12q13.3
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism
• MW: 36.4 kDa
• Gene Summary: The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]