CWF19L1 (NM_001303407) Human Tagged ORF Clone

CAT#: RC237246

CWF19L1 (myc-DDK-tagged) - Human CWF19-like 1, cell cycle control (S. pombe) (CWF19L1), transcript variant 5

Product Images

Circular map for RC237246

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CWF19L1
• Synonyms: C19L1; hDrn1; SCAR17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237246 representing NM_001303407
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTAATGGATGCAGCAGAACTGGTAAAACAGCCTCCGGATGTCACTGAAAACCCTTACAGAAAAT
  CTGGGCAGGAAGCATCCATAGGAAAGCAAATTCTTGCCCCTGTGGAAGAATCAGCCTGTCAGTTTTTCTT
  TGATTTAAATGAAAAGCAGGGAAGGAAGCGTTCATCCACAGGTAGAGATAGCAAATCTTCTCCTCATCCA
  AAGCAGCCTCGCAAACCTCCTCAGCCTCCAGGACCCTGCTGGTTTTGCCTTGCTAGCCCTGAAGTGGAAA
  AACATTTGGTGGTCAACATCGGCACACATTGCTACCTTGCCCTGGCCAAAGGAGGCTTATCTGATGACCA
  TGTCCTCATCCTGCCTATTGGACACTACCAGTCAGTGGTGGAGCTTTCAGCAGAGGTGGTAGAAGAGGTG
  GAGAAGTATAAGGCCACTCTGAGACGGTTCTTTAAGAGTCGAGGGAAATGGTGTGTTGTATTTGAGAGAA
  ATTATAAGAGCCATCACCTCCAGCTACAGGTCATTCCTGTCCCAATCAGCTGCTCTACTACTGATGACAT
  TAAAGATGCCTTCATTACCCAGGCACAGGAGCAGCAGATAGAGCTGTTGGAAATCCCAGAGCACTCTGAC
  ATCAAGCAGATTGCACAGCCAGGAGCAGCATATTTTTATGTTGAACTTGACACAGGAGAAAAGCTTTTCC
  ACAGAATTAAAAAGAATTTTCCTTTGCAGTTTGGAAGGGAGGTCCTGGCCAGTGAAGCCATCCTTAATGT
  TCCTGATAAGTCTGACTGGAGGCAGTGTCAGATCAGCAAGGAAGACGAGGAGACCCTGGCTCGCCGCTTC
  CGGAAAGACTTTGAGCCCTATGACTTTACTCTGGATGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237246 representing NM_001303407
  Red=Cloning site Green=Tags(s)
  
  MKLMDAAELVKQPPDVTENPYRKSGQEASIGKQILAPVEESACQFFFDLNEKQGRKRSSTGRDSKSSPHP
  KQPRKPPQPPGPCWFCLASPEVEKHLVVNIGTHCYLALAKGGLSDDHVLILPIGHYQSVVELSAEVVEEV
  EKYKATLRRFFKSRGKWCVVFERNYKSHHLQLQVIPVPISCSTTDDIKDAFITQAQEQQIELLEIPEHSD
  IKQIAQPGAAYFYVELDTGEKLFHRIKKNFPLQFGREVLASEAILNVPDKSDWRQCQISKEDEETLARRF
  RKDFEPYDFTLDD
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303407
• ORF Size: 879 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303407.2
• RefSeq Size: 2422 bp
• RefSeq ORF: 882 bp
• Locus ID: 55280
• Cytogenetics: 10q24.31
• MW: 33.9 kDa
• Gene Summary: This gene encodes a member of the CWF19 protein family. Mutations in this gene have been associated with autosomal recessive spinocerebellar ataxia-17 and mild cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]