NFAT5 (NM_173215) Human Tagged ORF Clone

CAT#: RC235309

NFAT5 (Myc-DDK tagged) - Homo sapiens nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant 5

Product Images

Circular map for RC235309

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NFAT5
• Synonyms: NF-AT5; NFATL1; NFATZ; OREBP; TONEBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235309 representing NM_173215
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGACA
  GCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAGCA
  GTTAACCAGTAACACAGTTCAGCAGCATCCATCAACACCGAAGAGGCACACAGTCTTGTACATCTCACCA
  CCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGTGTGGATTGGAATCTGAGC
  AGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAGCACCAGTTCCTACAATGA
  TAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAGAT
  TGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTTTCTCAGC
  TTACCACGGACAACAAAGGCAACTCAAAAGCGGGAAATGGAACATTGGAAAACCAAAAAGGAACTGGAGT
  AAAGAAGAGCCCTATGTTGTGTGGACAATATCCTGTTAAAAGTGAGGGAAAGGAGCTGAAGATAGTTGTA
  CAACCTGAGACACAGCACCGAGCTCGGTACCTGACTGAGGGCAGCCGTGGCTCAGTGAAAGATAGAACAC
  AGCAAGGCTTTCCTACAGTAAAGCTGGAAGGCCATAATGAACCTGTAGTGTTGCAAGTGTTTGTGGGCAA
  CGACTCTGGACGAGTGAAACCACATGGATTTTATCAGGCCTGCAGAGTAACTGGACGAAATACAACTCCT
  TGCAAAGAAGTGGACATTGAAGGCACTACTGTTATAGAAGTCGGCCTTGATCCTAGCAACAACATGACAC
  TGGCGGTGGACTGCGTAGGGATATTGAAATTGAGGAATGCTGATGTCGAAGCCAGAATAGGAATTGCTGG
  TTCCAAGAAGAAAAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCATGAGGAAAGATGGCTCCACT
  TTGACACTGCAAACACCCTCTTCTCCAATTTTGTGTACTCAGCCAGCAGGAGTGCCAGAAATCTTAAAGA
  AAAGCTTGCATAGCTGTTCAGTGAAAGGAGAAGAAGAAGTGTTTTTAATCGGCAAGAACTTTCTGAAAGG
  AACTAAAGTTATTTTCCAAGAAAATGTTTCTGATGAAAACTCTTGGAAGTCAGAAGCTGAAATTGATATG
  GAACTATTTCATCAGAATCATCTTATTGTGAAGGTTCCTCCCTATCATGACCAACATATAACTTTGCCTG
  TGTCAGTGGGAATATATGTAGTGACAAATGCTGGAAGATCTCATGATGTTCAACCATTCACTTACACTCC
  AGACCCAGCAGCAGCTGGTGCTTTGAATGTAAATGTGAAGAAGGAAATATCTAGTCCAGCAAGACCTTGC
  TCTTTTGAAGAGGCCATGAAAGCAATGAAAACTACTGGATGTAATTTAGATAAGGTAAATATTATCCCTA
  ATGCCCTGATGACTCCACTCATACCAAGCAGTATGATTAAGAGTGAAGATGTTACTCCAATGGAAGTAAC
  AGCAGAAAAAAGATCTTCCACTATTTTTAAGACTACAAAGTCTGTTGGATCAACTCAGCAAACATTAGAA
  AACATCTCAAACATAGCAGGAAATGGCTCTTTTTCATCACCATCATCTTCCCACCTACCTTCTGAAAATG
  AAAAACAGCAGCAGATTCAGCCCAAGGCATACAACCCAGAGACCCTGACAACTATTCAAACCCAGGACAT
  CTCACAGCCTGGTACTTTTCCAGCAGTTTCTGCTTCTAGTCAGCTGCCCAACAGCGATGCACTATTGCAG
  CAGGCTACACAGTTTCAGACAAGAGAAACTCAGTCTAGAGAGATATTACAGTCAGATGGTACAGTGGTTA
  ATTTGTCACAACTGACTGAGGCATCACAACAACAGCAGCAGTCACCACTACAAGAACAAGCACAGACTTT
  ACAGCAGCAGATTTCATCAAATATTTTTCCATCACCAAATAGTGTGAGTCAGCTTCAGAATACTATTCAG
  CAGCTGCAAGCAGGGAGTTTCACAGGCAGTACTGCTAGTGGCAGCAGTGGAAGTGTTGACTTGGTCCAAC
  AAGTTTTAGAGGCACAGCAGCAGTTATCTTCAGTTTTATTTTCTGCTCCAGATGGTAATGAGAATGTTCA
  AGAGCAGCTTAGTGCAGATATTTTTCAACAAGTCAGTCAAATTCAGAGTGGTGTAAGCCCTGGAATGTTT
  TCCTCAACAGAGCCAACAGTCCATACCAGACCAGATAATTTATTACCTGGAAGAGCTGAAAGTGTTCATC
  CACAGTCTGAAAACACGTTATCTAATCAACAGCAGCAGCAGCAGCAGCAACAGCAAGTGATGGAATCTTC
  AGCCGCAATGGTGATGGAGATGCAACAGAGTATCTGCCAGGCAGCTGCCCAGATTCAGTCAGAGTTATTC
  CCTTCAACTGCTTCAGCAAATGGAAACCTTCAGCAATCGCCAGTTTACCAGCAGACTTCTCACATGATGA
  GTGCATTGTCTACCAATGAGGATATGCAAATGCAGTGTGAATTGTTTTCTTCTCCTCCTGCAGTTTCTGG
  AAATGAAACTTCTACAACTACCACACAGCAGGTTGCAACCCCTGGCACTACCATGTTTCAGACATCAAGT
  TCAGGAGATGGAGAAGAAACTGGAACACAAGCAAAACAGATTCAGAACAGTGTCTTTCAGACCATGGTCC
  AAATGCAACATAGTGGGGACAATCAACCTCAAGTTAACCTTTTTTCATCCACAAAAAGTATGATGAGTGT
  TCAGAATAGTGGTACCCAACAACAAGGTAATGGTTTATTCCAGCAAGGGAATGAGATGATGTCACTTCAA
  TCTGGAAATTTTTTGCAGCAGTCTTCTCATTCACAGGCCCAACTTTTTCATCCTCAAAATCCTATTGCCG
  ATGCTCAGAACCTTTCCCAGGAAACTCAAGGTTCTCTCTTTCATAGTCCAAATCCTATTGTCCACAGTCA
  GACTTCTACAACCTCCTCTGAACAAATGCAGCCTCCAATGTTTCACTCTCAAAGTACCATTGCTGTGTTA
  CAGGGCTCTTCAGTTCCTCAAGACCAGCAGTCAACCAACATATTTCTTTCCCAGAGTCCCATGAATAATC
  TTCAGACTAACACAGTAGCCCAAGAAGCATTTTTTGCAGCACCGAACTCAATTTCTCCACTTCAGTCAAC
  ATCAAACAGTGAACAACAAGCTGCTTTCCAACAGCAAGCTCCAATATCACACATCCAGACTCCTATGCTT
  TCCCAAGAACAGGCACAACCCCCGCAGCAGGGTTTATTTCAGCCTCAGGTGGCCCTGGGCTCCCTTCCAC
  CTAATCCAATGCCTCAAAGCCAACAAGGAACCATGTTCCAGTCACAGCACTCAATAGTTGCCATGCAGAG
  TAACTCTCCATCCCAGGAACAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCAGCAGCAACAACAACAG
  AGCATTTTATTCAGTAATCAGAATACCATGGCTACAATGGCGTCTCCAAAGCAACCACCACCAAACATGA
  TATTCAACCCAAATCAAAATCCAATGGCTAATCAGGAGCAACAGAACCAGTCAATTTTTCACCAACAAAG
  TAACATGGCCCCAATGAATCAAGAGCAACAGCCCATGCAATTTCAGAGTCAGTCCACAGTTTCCTCACTT
  CAGAACCCAGGTCCTACCCAGTCGGAATCATCACAGACCCCCTTGTTCCATAGCTCTCCTCAGATTCAGT
  TGGTACAAGGGTCACCTAGTTCTCAAGAGCAGCAAGTAACTCTCTTCTTATCTCCAGCATCCATGTCTGC
  CTTGCAGACCAGTATAAATCAACAAGATATGCAACAGTCTCCTCTTTATTCCCCTCAGAACAACATGCCT
  GGAATTCAAGGAGCCACATCTTCGCCTCAACCACAGGCTACTTTATTTCACAACACAGCAGGAGGCACAA
  TGAACCAACTGCAGAATTCTCCTGGCTCATCTCAGCAGACATCAGGAATGTTCTTATTTGGCATTCAAAA
  TAACTGTAGTCAGCTTTTAACCTCTGGACCAGCTACATTGCCTGATCAGTTGATGGCCATAAGTCAGCCA
  GGCCAACCACAAAACGAGGGCCAGCCACCTGTGACAACACTTCTTTCTCAGCAAATGCCAGAGAATTCTC
  CACTGGCATCCTCTATAAACACCAACCAGAACATCGAAAAGATTGATTTGCTTGTTTCATTGCAAAACCA
  AGGGAACAACTTGACTGGCTCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235309 representing NM_173215
  Red=Cloning site Green=Tags(s)
  
  MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISP
  PPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRD
  CEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVV
  QPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTP
  CKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGST
  LTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDM
  ELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPC
  SFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLE
  NISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQ
  QATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQ
  QLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMF
  SSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELF
  PSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSS
  SGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQ
  SGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVL
  QGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPML
  SQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQ
  SILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSL
  QNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMP
  GIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQP
  GQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173215
• ORF Size: 4365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173215.3
• RefSeq Size: 5311 bp
• RefSeq ORF: 4368 bp
• Locus ID: 10725
• UniProt ID: O94916
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• MW: 158.4 kDa
• Gene Summary: The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]