CLINT1 (NM_001195556) Human Tagged ORF Clone

CAT#: RC234594

CLINT1 (Myc-DDK tagged) - Homo sapiens clathrin interactor 1 (CLINT1), transcript variant 3

Product Images

Circular map for RC234594

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLINT1
• Synonyms: CLINT; ENTH; EPN4; EPNR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234594 representing NM_001195556
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTATTCAGAGATCGAGTCTAAGGTTCGAGAGGCAACGAACGATGATCCTTGGGGACCTTCTGGGC
  AACTCATGGGAGAGATTGCCAAGGCTACATTTATGTATGAACAATTTCCAGAACTTATGAACATGCTTTG
  GTCACGAATGTTAAAAGACAACAAAAAGAATTGGAGAAGAGTTTATAAGTCGTTGCTGCTCCTAGCTTAC
  CTCATAAGGAATGGATCAGAGCGTGTTGTTACAAGTGCCAGAGAACACATTTATGATTTACGATCCCTGG
  AAAATTACCACTTTGTAGATGAGCATGGTAAGGATCAAGGTATAAATATTCGACAGAAGGTGAAGGAATT
  GGTTGAATTTGCCCAGGATGACGACAGGCTTCGTGAAGAGCGAAAGAAAGCAAAGAAGAACAAAGACAAG
  TATGTTGGGGTTTCCTCAGACAGTGTTGGAGGATTCAGATACAGTGAAAGATATGATCCTGAGCCCAAAT
  CAAAATGGGATGAGGAGTGGGATAAAAACAAGAGTGCTTTTCCATTCAGTGATAAATTAGGTGAGCTGAG
  TGATAAAATTGGAAGCACAATTGATGACACCATCAGCAAGTTCCGGAGGAAAGATAGAGAAGACTCTCCA
  GAAAGATGCAGCGACAGCGATGAGGAAAAGAAAGCGAGAAGAGGCAGATCTCCCAAAGGTGAATTCAAAG
  ATGAAGAGGAGACTGTGACGACAAAGCATATTCATATCACACAGGCCACAGAGACCACCACAACCAGACA
  CAAGCGCACAGCAAATCCTTCCAAAACCATTGATCTTGGAGCAGCAGCACATTACACAGGGGACAAAGCA
  AGTCCAGATCAGAATGCTTCAACCCACACACCTCAGTCTTCAGTTAAGACTTCAGTGCCTAGCAGCAAGT
  CATCTGGTGACCTTGTTGATCTGTTTGATGGCACCAGCCAGTCAACAGGAGGATCAGCTGATTTATTCGG
  AGGATTTGCTGACTTTGGCTCAGCTGCTGCATCAGGCAGTTTCCCTTCCCAAGTAACAGCAACAAGTGGG
  AATGGAGACTTTGGTGACTGGAGTGCCTTCAACCAAGCCCCATCAGGCCCTGTTGCTTCCAGTGGCGAGT
  TCTTTGGCAGTGCCTCACAGCCAGCGGTAGAACTTGTTAGTGGCTCACAATCAGCTCTAGGCCCACCTCC
  TGCTGCCTCAAATTCTTCAGACCTGTTTGATCTTATGGGCTCGTCCCAGGCAACCATGACATCTTCCCAG
  AGTATGAATTTCTCTATGATGAGCACTAACACTGTGGGACTTGGTTTGCCTATGTCAAGATCACAGCCTT
  TGCAAAATGTTAGCACAGTGCTGCAGAAGCCTAATCCTCTCTATAATCAGAATACAGATATGGTCCAGAA
  ATCAGTCAGCAAAACCTTGCCCTCTACTTGGTCTGACCCCAGTGTAAACATCAGCCTAGACAACTTACTA
  CCTGGTATGCAGCCTTCCAAACCCCAGCAGCCATCACTGAATACAATGATTCAGCAACAGAATATGCAGC
  AGCCTATGAATGTGATGACTCAAAGTTTTGGAGCTGTGAACCTCAGTTCTCCATCGAACATGCTTCCTGT
  CCGGCCCCAAACTAATGCTTTGATAGGGGGACCCATGCCTATGAGCATGCCCAATGTGATGACTGGCACC
  ATGGGAATGGCCCCTCTTGGAAATACTCCGATGATGAACCAGAGCATGATGGGCATGAACATGAACATAG
  GGATGTCCGCTGCTGGGATGGGCTTGACAGGCACAATGGGAATGGGCATGCCCAACATAGCCATGACTTC
  TGGAACTGTGCAACCCAAGCAAGATGCCTTTGCAAATTTCGCCAATTTTAGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234594 representing NM_001195556
  Red=Cloning site Green=Tags(s)
  
  MNYSEIESKVREATNDDPWGPSGQLMGEIAKATFMYEQFPELMNMLWSRMLKDNKKNWRRVYKSLLLLAY
  LIRNGSERVVTSAREHIYDLRSLENYHFVDEHGKDQGINIRQKVKELVEFAQDDDRLREERKKAKKNKDK
  YVGVSSDSVGGFRYSERYDPEPKSKWDEEWDKNKSAFPFSDKLGELSDKIGSTIDDTISKFRRKDREDSP
  ERCSDSDEEKKARRGRSPKGEFKDEEETVTTKHIHITQATETTTTRHKRTANPSKTIDLGAAAHYTGDKA
  SPDQNASTHTPQSSVKTSVPSSKSSGDLVDLFDGTSQSTGGSADLFGGFADFGSAAASGSFPSQVTATSG
  NGDFGDWSAFNQAPSGPVASSGEFFGSASQPAVELVSGSQSALGPPPAASNSSDLFDLMGSSQATMTSSQ
  SMNFSMMSTNTVGLGLPMSRSQPLQNVSTVLQKPNPLYNQNTDMVQKSVSKTLPSTWSDPSVNISLDNLL
  PGMQPSKPQQPSLNTMIQQQNMQQPMNVMTQSFGAVNLSSPSNMLPVRPQTNALIGGPMPMSMPNVMTGT
  MGMAPLGNTPMMNQSMMGMNMNIGMSAAGMGLTGTMGMGMPNIAMTSGTVQPKQDAFANFANFSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195556
• ORF Size: 1875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195556.2
• RefSeq Size: 4038 bp
• RefSeq ORF: 1878 bp
• Locus ID: 9685
• UniProt ID: Q14677
• Cytogenetics: 5q33.3
• MW: 68.6 kDa
• Gene Summary: This gene encodes a protein with similarity to the epsin family of endocytic adapter proteins. The encoded protein interacts with clathrin, the adapter protein AP-1 and phosphoinositides. This protein may be involved in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in this gene are associated with a susceptibility to schizophrenia and psychotic disorders. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]