SRP68 (NM_001260502) Human Tagged ORF Clone

CAT#: RC234506

SRP68 (Myc-DDK tagged) - Homo sapiens signal recognition particle 68kDa (SRP68), transcript variant 2

Product Images

Circular map for RC234506

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRP68
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234506 representing NM_001260502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGAGAAGCAGGTCCCAGGCGGCGGCGGCGGCGGCGGCAGTGGCGGCGGCGGTGGCAGTGGCG
  GCGGCGGTAGCGGCGGTGGACGTGGTGCCGGAGGGGAAGAAAATAAAGAAAACGAACGCCCTTCGGCCGG
  ATCGAAGGCAAACAAAGAATTTGGGGATAGCCTGAGTTTGGAGATTCTTCAGATTATTAAGGAATCCCAG
  CAGCAGCATGGTTTACGGCATGGAGATTTTCAGAGGTACAGATACTTGCTTCTGGTTCTGATGGATGCTG
  AAAGAGCCTGGAGCTACGCCATGCAGCTGAAACAGGAAGCCAACACTGAACCCCGAAAACGGTTTCACTT
  GTTATCTCGCCTACGCAAAGCCGTGAAGCATGCAGAGGAATTGGAACGCTTGTGTGAGAGCAATCGCGTG
  GATGCCAAGACCAAATTAGAGGCTCAGGCTTACACAGCTTACCTCTCAGGAATGCTACGTTTTGAACATC
  AAGAATGGAAAGCTGCCATTGAGGCTTTTAACAAATGCAAAACTATCTATGAGAAGCTAGCCAGTGCTTT
  CACAGAGGAGCAGGCTGTGCTGTATAACCAACGTGTGGAAGAGATTTCACCCAACATCCGCTATTGTGCA
  TATAATATTGGGGACCAGTCAGCCATCAATGAACTCATGCAGATGAGATTGAGGTCTGGGGGCACTGAGG
  GTCTCTTGGCTGAAAAATTGGAGGCTTTGATCACTCAGACTCGAGCCAAACAGGCAGCTACCATGAGTGA
  AGTGGAGTGGAGAGGGAGAACGGTTCCAGTGAAGATTGACAAAGTGCGCATTTTCTTATTAGGACTGGCT
  GATAACGAAGCAGCTATTGTCCAGGCTGAAAGCGAAGAAACTAAGGAGCGCCTGTTTGAATCAATGCTCA
  GCGAGTGTCGGGACGCCATCCAGGTGGTTCGGGAGGAGCTCAAGCCAGATCAGAAACAGAGAGATTATAT
  CCTTGAAGGAGAGCCAGGGAAGGTGTCTAATCTTCAATACTTGCATAGCTACCTGACTTACATCAAGCTA
  TCAACGGCAATCAAGCGTAATGAGAACATGGCCAAAGGTCTGCAGAGGGCTCTGCTGCAGCAGCAGCCAG
  AGGATGACAGCAAGCGCTCACCCCGGCCCCAGGACCTGATCCGACTCTATGACATCATCTTACAGAATCT
  GGTGGAATTGCTCCAGCTTCCTGGTTTAGAGGAAGACAAAGCCTTCCAGAAAGAGATAGGCCTCAAGACT
  CTGGTGTTCAAAGCTTACAGGTGTTTTTTCATTGCTCAGTCCTATGTGCTGGTGAAGAAGTGGAGCGAAG
  CCCTTGTCCTGTATGACAGAGTCCTGAAATATGCAAATGAAGTAAATTCTGATGCTGGCGCCTTCAAGAA
  CAGCCTAAAGGACCTGCCTGATGTGCAAGAGCTCATCACTCAAGTGCGGTCAGAGAAGTGCTCCCTGCAG
  GCCGCAGCCATCCTTGATGCAAACGACGCTCATCAAACAGAGACCTCCTCCTCCCAAGTCAAGGACAATA
  AGCCTCTGGTTGAACGGTTTGAGACATTCTGCCTGGACCCTTCCCTTGTCACCAAGCAAGCCAACCTTGT
  GCACTTCCCACCAGGCTTCCAGCCCATTCCCTGCAAGCCTTTGTTCTTTGACCTGGCCCTCAACCATGTG
  GCTTTCCCACCCCTTGAGGACAAGTTGGAACAGAAGACCAAGAGTGGCCTCACTGGATACATCAAGGGCA
  TCTTTGGATTCAGGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234506 representing NM_001260502
  Red=Cloning site Green=Tags(s)
  
  MAAEKQVPGGGGGGGSGGGGGSGGGGSGGGRGAGGEENKENERPSAGSKANKEFGDSLSLEILQIIKESQ
  QQHGLRHGDFQRYRYLLLVLMDAERAWSYAMQLKQEANTEPRKRFHLLSRLRKAVKHAEELERLCESNRV
  DAKTKLEAQAYTAYLSGMLRFEHQEWKAAIEAFNKCKTIYEKLASAFTEEQAVLYNQRVEEISPNIRYCA
  YNIGDQSAINELMQMRLRSGGTEGLLAEKLEALITQTRAKQAATMSEVEWRGRTVPVKIDKVRIFLLGLA
  DNEAAIVQAESEETKERLFESMLSECRDAIQVVREELKPDQKQRDYILEGEPGKVSNLQYLHSYLTYIKL
  STAIKRNENMAKGLQRALLQQQPEDDSKRSPRPQDLIRLYDIILQNLVELLQLPGLEEDKAFQKEIGLKT
  LVFKAYRCFFIAQSYVLVKKWSEALVLYDRVLKYANEVNSDAGAFKNSLKDLPDVQELITQVRSEKCSLQ
  AAAILDANDAHQTETSSSQVKDNKPLVERFETFCLDPSLVTKQANLVHFPPGFQPIPCKPLFFDLALNHV
  AFPPLEDKLEQKTKSGLTGYIKGIFGFRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001260502
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001260502.1, NP_001247431.1
• RefSeq Size: 2736 bp
• RefSeq ORF: 1770 bp
• Locus ID: 6730
• UniProt ID: Q9UHB9
• Cytogenetics: 17q25.1
• Protein Pathways: Protein export
• MW: 66.6 kDa
• Gene Summary: This gene encodes a subunit of the signal recognition particle (SRP). The SRP is a ribonucleoprotein complex that transports secreted and membrane proteins to the endoplasmic reticulum for processing. The complex includes a 7S RNA and six protein subunits. The encoded protein is the 68kDa component of the SRP, and forms a heterodimer with the 72kDa subunit that is required for SRP function. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and three pseudogenes of this gene are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, May 2012]