STK3 (NM_001256312) Human Tagged ORF Clone

CAT#: RC234335

STK3 (Myc-DDK tagged) - Homo sapiens serine/threonine kinase 3 (STK3), transcript variant 2

Product Images

Circular map for RC234335

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STK3
• Synonyms: KRS1; MST2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234335 representing NM_001256312
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTACAACTCATGGACAGTGGCATTACCATCTGCCTTAGGAACGGAGCAGCCTCTGTCTTCAAGAAAA
  AAGAATGGAGCACACAAGGAGAAGAAAACAAGCAAGACAGTAAACTAAAAAAGCTGAGTGAAGACAGTTT
  GACTAAGCAGCCTGAAGAAGTTTTTGATGTATTAGAGAAGCTTGGAGAAGGGTCTTATGGAAGTGTATTT
  AAAGCAATACACAAGGAATCCGGTCAAGTTGTCGCAATTAAACAAGTACCTGTTGAATCAGATCTTCAGG
  AAATAATCAAAGAAATTTCCATAATGCAGCAATGTGACAGCCCATATGTTGTAAAGTACTATGGCAGTTA
  TTTTAAGAATACAGACCTCTGGATTGTTATGGAGTACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGA
  TTACGAAACAAGACATTAATAGAAGATGAAATTGCAACCATTCTTAAATCTACATTGAAAGGACTAGAAT
  ATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGAAATATTCTCCTCAATACAGAAGGACA
  TGCAAAATTGGCAGATTTTGGAGTGGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATACTGTAATA
  GGAACTCCATTTTGGATGGCTCCTGAGGTGATTCAAGAAATAGGCTATAACTGTGTGGCCGACATCTGGT
  CCCTTGGCATTACTTCTATAGAAATGGCTGAAGGAAAACCTCCTTATGCTGATATACATCCAATGAGGGC
  TATTTTTATGATTCCCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACC
  GATTTTGTTAAAAAGTGTTTGGTGAAGAATCCTGAGCAGAGAGCTACTGCAACACAACTTTTACAGCATC
  CTTTTATCAAGAATGCCAAACCTGTATCAATATTAAGAGACCTGATCACAGAAGCTATGGAGATCAAAGC
  TAAAAGACATGAGGAACAGCAACGAGAATTGGAAGAGGAAGAAGAAAATTCGGATGAAGATGAGCTGGAT
  TCCCACACCATGGTGAAGACTAGTGTGGAGAGTGTGGGCACCATGCGGGCCACAAGCACGATGAGTGAAG
  GGGCCCAGACCATGATTGAACATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGATAAACAG
  TGAGGATGAGGAAGAAGAAGATGGAACTATGAAAAGAAATGCAACCTCACCACAAGTACAAAGACCATCT
  TTCATGGACTACTTTGATAAGCAAGACTTCAAGAATAAGAGTCACGAAAACTGTAATCAGAACATGCATG
  AACCCTTCCCTATGTCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCTCAAGATGGAGACTTTGACTT
  TTTGAAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTAAAAGCACTGGACCCCATGATGGAACGGGAG
  ATAGAAGAACTTCGTCAGAGATACACTGCGAAAAGACAGCCCATTCTGGATGCGATGGATGCAAAGAAAA
  GAAGGCAGCAAAACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234335 representing NM_001256312
  Red=Cloning site Green=Tags(s)
  
  MLQLMDSGITICLRNGAASVFKKKEWSTQGEENKQDSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVF
  KAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIR
  LRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVI
  GTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFT
  DFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELD
  SHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPS
  FMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMERE
  IEELRQRYTAKRQPILDAMDAKKRRQQNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256312
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256312.2
• RefSeq Size: 3171 bp
• RefSeq ORF: 1560 bp
• Locus ID: 6788
• UniProt ID: Q13188
• Cytogenetics: 8q22.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• MW: 59.9 kDa
• Gene Summary: This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]