SPAG6 (NM_001253854) Human Tagged ORF Clone

CAT#: RC234239

SPAG6 (Myc-DDK tagged) - Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 3

Product Images

Circular map for RC234239

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPAG6
• Synonyms: CFAP194; CT141; FAP194; pf16; Repro-SA-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234239 representing NM_001253854
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTCACTCACCATGGATCTCTGGATACCTAATGGACAGGCAGGTGTAATGTCTTTGCTGAGAACTC
  TTCTTCTGGACGTGGTCCCAACAATTCAACAGACTGCTGCTTTGGCTCTTGGGAGACTGGCCAATTATAA
  TGATGACCTAGCAGAAGCTGTTGTGAAGTGCGACATTCTTCCACAGCTTGTTTATTCATTGGCAGAACAG
  AATCGCTTCTACAAGAAAGCAGCTGCCTTTGTGTTACGAGCAGTTGGTAAACATTCTCCCCAGCTAGCTC
  AGGCAATAGTCGATTGTGGAGCACTGGATACGCTGGTCATATGCTTGGAAGATTTTGACCCTGGAGTCAA
  GGAGGCTGCAGCCTGGGCACTTAGATATATTGCAAGACATAATGCAGAACTGTCACAAGCTGTGGTGGAT
  GCAGGAGCTGTTCCTCTTTTAGTACTCTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATTGCTGCTT
  CGGCCCTCAGTGATATTGCAAAGCATTCTCCAGAGTTAGCACAGACAGTAGTGGATGCAGGAGCTGTTGC
  TCATTTAGCCCAGATGATCCTGAACCCTGATGCTAAATTGAAGCATCAGATCCTTTCAGCTCTCAGTCAG
  GTTTCAAAACATTCCGTGGATCTGGCAGAAATGGTTGTTGAAGCAGAGATTTTTCCAGTTGTACTTACCT
  GTCTGAAGGACAAGGATGAATACGTGAAGAAAAATGCTTCTACTTTAATTAGAGAGATTGCAAAACATAC
  ACCCGAGCTTTCACAGCTGGTAGTTAACGCAGGAGGGGTTGCTGCCGTGATTGACTGCATTGGGTCCTGC
  AAAGGGAACACACGGCTGCCTGGCATCATGATGCTTGGTTATGTAGCAGCTCATTCTGAGAACCTAGCAA
  TGGCAGTCATCATTTCTAAGGGTGTACCCCAGTTGTCAGTCTGCTTGTCAGAAGAACCGGAAGATCATAT
  TAAGGCTGCAGCTGCTTGGGCCTTAGGACAGATTGGAAGACACACTCCTGAACACGCACGGGCTGTTGCA
  GTCACAAATACTTTGCCAGTTCTGCTTTCTTTGTACATGTCAACAGAAAGTTCTGAGGATCTCCAAGTAA
  AAAGTAAAAAAGCCATAAAGAATATCCTGCAAAAATGTACCTACTTACCAGCCCTTGAACCATTTCTATA
  TGATGCTCCTCCCAATATTCTGAAACATGTGGTTGGACAGTTCAGTAAGGTGCTGCCGCATGATAGCAAA
  GCTCGACGACTTTTTGTAACAAGTGGTGGCCTTAAAAAAGTTCAAGAGATAAAAGCAGAACCTGGTTCTC
  TCCTTCAAGAATACATCAACAGTATTAACAGTTGTTACCCCGAGGAAATAGTGAGGTATTATTCCCCTGG
  ATATTCAGATACACTTCTGCAGAGGGTGGACAGCTATCAACCACTTAATAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234239 representing NM_001253854
  Red=Cloning site Green=Tags(s)
  
  MHSLTMDLWIPNGQAGVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQ
  NRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVD
  AGAVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGAVAHLAQMILNPDAKLKHQILSALSQ
  VSKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNASTLIREIAKHTPELSQLVVNAGGVAAVIDCIGSC
  KGNTRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSVCLSEEPEDHIKAAAAWALGQIGRHTPEHARAVA
  VTNTLPVLLSLYMSTESSEDLQVKSKKAIKNILQKCTYLPALEPFLYDAPPNILKHVVGQFSKVLPHDSK
  ARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINSCYPEEIVRYYSPGYSDTLLQRVDSYQPLNN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253854
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253854.2
• RefSeq Size: 2989 bp
• RefSeq ORF: 1455 bp
• Locus ID: 9576
• UniProt ID: O75602
• Cytogenetics: 10p12.2
• MW: 52.9 kDa
• Gene Summary: The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]