DEF8 (NM_001242816) Human Tagged ORF Clone

CAT#: RC234103

DEF8 (Myc-DDK tagged) - Homo sapiens differentially expressed in FDCP 8 homolog (mouse) (DEF8), transcript variant 3

Product Images

Circular map for RC234103

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DEF8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234103 representing NM_001242816
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATATGATGAGAAGCTGGCCCGTTTCCGGCAGGCCCACCTCAACCCCTTCAACAAGCAGTCTGGGC
  CGAGACAGCATGAGCAGGGCCCTGGGGAGGAGGTCCCGGACGTCACTCCTGAAGAGGCCCTGCCTGAGCT
  GCCCCCTGGGGAGCCGGAATTCCGCTGCCCTGAACGCGTGATGGATCTCGGCCTGTCTGAGGACCACTTC
  TCCCGCCCTGTGCTGCGGCAGGCGATCGAGGAGTGCAAGCAGGTGATTCTGGAGCTGCCCGAGCAGTCGG
  AGAAGCAGAAGGATGCCGTGGTGCGACTCATCCACCTCCGGCTGAAGCTCCAGGAGCTGAAGGACCCCAA
  TGAGGATGAGCCAAACATCCGAGTGCTCCTTGAGCACCGCTTTTACAAGGAGAAGAGCAAGAGCGTCAAG
  CAGACCTGTGACAAGTGTAACACCATCATCTGGGGGCTCATTCAGACCTGGTACACCTGCACAGGGTGTT
  ATTACCGCTGTCACAGTAAGTGCTTGAACCTCATCTCCAAGCCCTGTGTGAGCTCCAAAGTCAGCCACCA
  AGCTGAATACGAACTGAACATCTGCCCTGAGACAGGGCTGGACAGCCAGGATTACCGCTGTGCCGAGTGC
  CGGGCGCCCATCTCTCTGCGGGGTGTGCCCAGTGAGGCCAGGCAGTGCGACTACACCGGCCAGTACTACT
  GCAGCCACTGCCACTGGAACGACCTGGCTGTGATCCCTGCACGCGTTGTACACAACTGGGACTTTGAGCC
  TCGAAAGGTTTCTCGCTGCAGCATGCGCTACCTGGCGCTGATGGTGTCTCGGCCCGTACTCAGGCTCCGG
  GAGATCAACCCTCTGCTGTTCAGCTACGTGGAGGAGCTGGTGGAGATTCGCAAGCTGCGCCAGGACATCC
  TGCTCATGAAGCCGTACTTCATCACCTGCAGGGAGGCCATGGAGGCTCGTCTGCTGCTGCAGCTCCAGGA
  TCGGCAGCATTTTGTGGAGAACGACGAGATGTACTCTGTCCAGGACCTCCTGGACGTGCATGCCGGCCGC
  CTGGGCTGCTCGCTCACCGAGATCCACACGCTCTTCGCCAAGCACATCAAGCTGGACTGCGAGCGGTGCC
  AGGCCAAGGGCTTCGTGTGTGAGCTCTGCAGAGAGGGCGACGTGCTGTTCCCGTTCGACAGCCACACGTC
  TGTGTGCGCCGACTGCTCCGCGGTCTTCCACAGGGACTGCTACTACGACAACTCCACCACTTGTCCCAAG
  TGTGCCCGGCTCAGCCTGAGGAAGCAGTCGCTCTTCCAGGAGCCAGGTCCCGATGTGGAGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234103 representing NM_001242816
  Red=Cloning site Green=Tags(s)
  
  MEYDEKLARFRQAHLNPFNKQSGPRQHEQGPGEEVPDVTPEEALPELPPGEPEFRCPERVMDLGLSEDHF
  SRPVLRQAIEECKQVILELPEQSEKQKDAVVRLIHLRLKLQELKDPNEDEPNIRVLLEHRFYKEKSKSVK
  QTCDKCNTIIWGLIQTWYTCTGCYYRCHSKCLNLISKPCVSSKVSHQAEYELNICPETGLDSQDYRCAEC
  RAPISLRGVPSEARQCDYTGQYYCSHCHWNDLAVIPARVVHNWDFEPRKVSRCSMRYLALMVSRPVLRLR
  EINPLLFSYVEELVEIRKLRQDILLMKPYFITCREAMEARLLLQLQDRQHFVENDEMYSVQDLLDVHAGR
  LGCSLTEIHTLFAKHIKLDCERCQAKGFVCELCREGDVLFPFDSHTSVCADCSAVFHRDCYYDNSTTCPK
  CARLSLRKQSLFQEPGPDVEA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242816
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242816.2
• RefSeq Size: 3632 bp
• RefSeq ORF: 1326 bp
• Locus ID: 54849
• UniProt ID: Q6ZN54
• Cytogenetics: 16q24.3
• MW: 51.7 kDa
• Gene Summary: Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts. Involved in bone resorption.[UniProtKB/Swiss-Prot Function]