RAB34 (NM_001256281) Human Tagged ORF Clone

CAT#: RC233414

NARR (Myc-DDK tagged) - Homo sapiens nine-amino acid residue-repeats (NARR)

Product Images

Circular map for RC233414

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAB34
• Synonyms: NARR; RAB39; RAH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233414 representing NM_001256281
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGGGCAGCCTCAGCCCCGAGATGACGTCGGGTCTCCGCGGCCCCGGGTTATTGTAGGAACCATCC
  GGCCCCGCGTAATCGTAGGAACTATCCGGCCCCGCGTAATCGTAGGGTCTGCGCGGGCCCGGCCCCCACC
  AGACGGGACTCCCCGCCCCCAATTGGCGGCCGAAGAGTCTCCTCGCCCCAGAGTCATCTTCGGGACGCCC
  AGGGCCCGGGTGATTTTGGGCTCGCCGCGGCCCCGGGTGATTGTTTCATCTCCGTGGCCCGCGGTGGTCG
  TAGCGTCTCCGAGACCGCGGACTCCCGTAGGGTCCCCGTGGCCCCGAGTTGTAGTCGGGACACCCCGGCC
  GCGGGTGATCGTCGGGTCTCCACGCGCCCGGGTCGCTGACGCGGATCCGGCCTCGGCGCCTTCTCAGGGC
  GCCCTGCAAGGCCGCAGGCAGGATGAACATTCTGGCACCCGTGCGGAGGGATCGCGTCCTGGCGGAGCTG
  CCCCAGTGCCTGAGGAAGGAGGCCGCTTTGCACGGGCACAAAGACTTCCACCCCCGCGTCACCTGCGCCT
  GCCAGGAGCACCGGACAGGCACCGTGGGCAGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233414 representing NM_001256281
  Red=Cloning site Green=Tags(s)
  
  MVGQPQPRDDVGSPRPRVIVGTIRPRVIVGTIRPRVIVGSARARPPPDGTPRPQLAAEESPRPRVIFGTP
  RARVILGSPRPRVIVSSPWPAVVVASPRPRTPVGSPWPRVVVGTPRPRVIVGSPRARVADADPASAPSQG
  ALQGRRQDEHSGTRAEGSRPGGAAPVPEEGGRFARAQRLPPPRHLRLPGAPDRHRGQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256281
• ORF Size: 594 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256281.3
• RefSeq Size: 839 bp
• RefSeq ORF: 597 bp
• Locus ID: 83871
• UniProt ID: P0DI83
• Cytogenetics: 17q11.2
• Protein Families: Druggable Genome
• MW: 21.6 kDa
• Gene Summary: This gene encodes a protein belonging to the RAB family of proteins, which are small GTPases involved in protein transport. This family member is a Golgi-bound member of the secretory pathway that is involved in the repositioning of lysosomes and the activation of macropinocytosis. Alternative splicing of this gene results in multiple transcript variants. An alternatively spliced transcript variant produces the nine-amino acid residue-repeats (NARR) protein, which is a functionally distinct nucleolar protein resulting from a different reading frame. [provided by RefSeq, Dec 2016]