RGL2 (NM_001243738) Human Tagged ORF Clone

CAT#: RC233087

RGL2 (Myc-DDK tagged) - Homo sapiens ral guanine nucleotide dissociation stimulator-like 2 (RGL2), transcript variant 2

Product Images

Circular map for RC233087

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RGL2
• Synonyms: HKE1.5; KE1.5; RAB2L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233087 representing NM_001243738
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCCCCACGTTCCTCCCGACGGCTCCGAGCTGGCACTCTGGAGGCCCTGGTCAGACACCTACTGG
  ATACCCGGACATCAGGGACTGATGTGAGCTTCATGTCAGCCTTCCTGGCTACCCACCGGGCCTTCACCTC
  CACGCCTGCCTTGCTAGGGCTTATGGCTGACAGGCTGGAAGCCCTTGAATCTCATCCTACCGACGAACTA
  GAGAGGACAACAGAGGTAGCCATCTCTGTACTGTCAACCTGGCTGGCCTCTCACCCTGAGGATTTTGGCT
  CTGAGGCCAAGGGTCAGCTTGACCGGCTTGAGAGCTTCTTACTTCAGACAGGGTATGCAGCAGGGAAGGG
  TGTTGGGGGGGGCAGCGCTGACCTCATCCGCAATCTCCGGTCCCGGGTGGACCCCCAGGCCCCCGACCTT
  CCTAAGCCCCTGGCCCTCCCCGGCGATCCCCCTGCTGACCCCACGGATGTCCTGGTGTTCCTCGCTGACC
  ACTTGGCCGAACAGCTGACCCTGCTAGATGCGGAACTTTTTCTCAATTTGATCCCCTCTCAGTGCCTGGG
  AGGCCTGTGGGGTCACAGAGACCGGCCAGGACATTCTCACCTCTGCCCATCTGTCCGAGCTACTGTCACA
  CAGTTTAACAAGGTGGCAGGGGCAGTGGTTAGTTCTGTCCTGGGGGCTACTTCCACTGGAGAGGGACCTG
  GGGAGGTGACCATACGGCCACTCCGTCCCCCACAGAGGGCCCGGCTCCTGGAGAAGTGGATCCGCGTGGC
  AGAGGAGTGCCGGCTGCTCCGAAACTTCTCTTCAGTTTATGCCGTGGTGTCAGCCCTGCAGTCCAGCCCC
  ATCCACAGGCTTCGGGCAGCCTGGGGGGAAGCAACCAGGGACAGCCTCAGAGTCTTTTCCAGCCTCTGCC
  AGATTTTCTCCGAGGAGGATAATTATTCCCAGAGTCGGGAGCTGCTCGTGCAGGAGGTGAAGCTGCAGTC
  TCCTCTGGAGCCACACTCCAAGAAGGCCCCGAGGTCTGGCTCCCGGGGTGGGGGTGTGGTCCCATACCTT
  GGCACCTTCCTGAAGGACCTTGTGATGCTGGATGCAGCCTCCAAGGATGAGTTGGAGAATGGATACATCA
  ATTTTGACAAGCGGAGGAAGGAGTTTGCAGTCCTTTCTGAGTTGCGACGGCTCCAGAATGAATGTCGTGG
  CTATAACCTCCAACCTGACCATGATATCCAGAGGTGGCTACAGGGGCTCCGGCCACTGACAGAGGCTCAG
  AGCCATCGTGTATCCTGTGAGGTGGAGCCACCTGGTTCCAGTGACCCTCCTGCCCCACGGGTGCTTCGGC
  CAACATTGGTCATCTCGCAGTGGACAGAGGTTTTGGGCTCTGTTGGGGTCCCTACCCCGCTTGTGTCCTG
  TGACCGGCCCAGTACTGGGGGAGATGAGGCGCCTACAACTCCTGCTCCTCTGCTGACTCGGCTGGCCCAG
  CACATGAAGTGGCCATCTGTCTCGTCACTAGACTCTGCCTTGGAAAGCAGTCCATCCCTGCACAGTCCAG
  CTGACCCCAGCCACCTCTCCCCACCAGCCTCCTCCCCTAGGCCTTCTCGAGGTCACCGCCGCTCAGCCTC
  CTGTGGCTCCCCGCTGAGTGGGGGTGCAGAAGAGGCCTCCGGGGGGACTGGATATGGGGGAGAGGGATCT
  GGGCCAGGGGCCTCTGATTGCCGTATCATCCGAGTCCAGATGGAGTTGGGGGAAGATGGCAGTGTCTATA
  AGAGCATTTTGGTGACAAGCCAGGACAAGGCTCCAAGTGTCATCAGTCGTGTCCTTAAGAAAAACAATCG
  TGACTCTGCAGTGGCTTCAGAGTATGAGCTGGTACAGCTGCTACCAGGGGAGCGAGAGCTGACTATCCCA
  GCCTCGGCTAATGTATTCTACGCCATGGATGGAGCTTCACACGATTTCCTCCTGCGGCAGCGGCGAAGGT
  CCTCTACTGCTACACCTGGCGTCACCAGTGGCCCGTCTGCCTCAGGAACTCCTCCGAGTGAGGGAGGAGG
  GGGCTCCTTTCCCAGGATCAAGGCCACAGGGAGGAAGATTGCACGGGCACTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233087 representing NM_001243738
  Red=Cloning site Green=Tags(s)
  
  MPPPRSSRRLRAGTLEALVRHLLDTRTSGTDVSFMSAFLATHRAFTSTPALLGLMADRLEALESHPTDEL
  ERTTEVAISVLSTWLASHPEDFGSEAKGQLDRLESFLLQTGYAAGKGVGGGSADLIRNLRSRVDPQAPDL
  PKPLALPGDPPADPTDVLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGHSHLCPSVRATVT
  QFNKVAGAVVSSVLGATSTGEGPGEVTIRPLRPPQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSP
  IHRLRAAWGEATRDSLRVFSSLCQIFSEEDNYSQSRELLVQEVKLQSPLEPHSKKAPRSGSRGGGVVPYL
  GTFLKDLVMLDAASKDELENGYINFDKRRKEFAVLSELRRLQNECRGYNLQPDHDIQRWLQGLRPLTEAQ
  SHRVSCEVEPPGSSDPPAPRVLRPTLVISQWTEVLGSVGVPTPLVSCDRPSTGGDEAPTTPAPLLTRLAQ
  HMKWPSVSSLDSALESSPSLHSPADPSHLSPPASSPRPSRGHRRSASCGSPLSGGAEEASGGTGYGGEGS
  GPGASDCRIIRVQMELGEDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVASEYELVQLLPGERELTIP
  ASANVFYAMDGASHDFLLRQRRRSSTATPGVTSGPSASGTPPSEGGGGSFPRIKATGRKIARALF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243738
• ORF Size: 2085 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243738.1, NP_001230667.1
• RefSeq Size: 3165 bp
• RefSeq ORF: 2088 bp
• Locus ID: 5863
• UniProt ID: O15211
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome
• MW: 75.2 kDa
• Gene Summary: Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro (By similarity).[UniProtKB/Swiss-Prot Function]