MELK (NM_001256688) Human Tagged ORF Clone

CAT#: RC232990

MELK (Myc-DDK tagged) - Homo sapiens maternal embryonic leucine zipper kinase (MELK), transcript variant 4

Product Images

Circular map for RC232990

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MELK
• Synonyms: HPK38
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232990 representing NM_001256688
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGATTATGATGAACTTCTCAAATATTATGAATTACATGAAACTATTGGGACAGGTGGCTTTGCAA
  AGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTAGCTATAAAAATCATGGATAAAAACACACT
  AGGGAGTGATTTGCCCCGGATCAAAACGGAGATTGAGGCCTTGAAGAACCTGAGACATCAGCATATATGT
  CAACTCTACCATGTGCTAGAGACAGCCAACAAAATATTCATGGTTCTTGAGGGTAACAAGGATTACCATC
  TACAGACATGCTGTGGGAGTCTGGCTTATGCAGCACCTGAGTTAATACAAGGCAAATCATATCTTGGATC
  AGAGGCAGATGTTTGGAGCATGGGCATACTGTTATATGTTCTTATGTGTGGATTTCTACCATTTGATGAT
  GATAATGTAATGGCTTTATACAAGAAGATTATGAGAGGAAAATATGATGTTCCCAAGTGGCTCTCTCCCA
  GTAGCATTCTGCTTCTTCAACAAATGCTGCAGGTGGACCCAAAGAAACGGATTTCTATGAAAAATCTATT
  GAACCATCCCTGGATCATGCAAGATTACAACTATCCTGTTGAGTGGCAAAGCAAGAATCCTTTTATTCAC
  CTCGATGATGATTGCGTAACAGAACTTTCTGTACATCACAGAAACAACAGGCAAACAATGGAGGATTTAA
  TTTCACTGTGGCAGTATGATCACCTCACGGCTACCTATCTTCTGCTTCTAGCCAAGAAGGCTCGGGGAAA
  ACCAGTTCGTTTAAGGCTTTCTTCTTTCTCCTGTGGACAAGCCAGTGCTACCCCATTCACAGACATCAAG
  TCAAATAATTGGAGTCTGGAAGATGTGACCGCAAGTGATAAAAATTATGTGGCGGGATTAATAGACTATG
  ATTGGTGTGAAGATGATTTATCAACAGGTGCTGCTACTCCCCGAACATCACAGTTTACCAAGTACTGGAC
  AGAATCAAATGGGGTGGAATCTAAATCATTAACTCCAGCCTTATGCAGAACACCTGCAAATAAATTAAAG
  AACAAAGAAAATGTATATACTCCTAAGTCTGCTGTAAAGAATGAAGAGTACTTTATGTTTCCTGAGCCAA
  AGACTCCAGTTAATAAGAACCAGCATAAGAGAGAAATACTCACTACGCCAAATCGTTACACTACACCCTC
  AAAAGCTAGAAACCAGTGCCTGAAAGAAACTCCAATTAAAATACCAGTAAATTCAACAGGAACAGACAAG
  TTAATGACAGGTGTCATTAGCCCTGAGAGGCGGTGCCGCTCAGTGGAATTGGATCTCAACCAAGCACATA
  TGGAGGAGACTCCAAAAAGAAAGGGAGCCAAAGTGTTTGGGAGCCTTGAAAGGGGGTTGGATAAGGTTAT
  CACTGTGCTCACCAGGAGCAAAAGGAAGGGTTCTGCCAGAGACGGGCCCAGAAGACTAAAGCTTCACTAT
  AACGTGACTACAACTAGATTAGTGAATCCAGATCAACTGTTGAATGAAATAATGTCTATTCTTCCAAAGA
  AGCATGTTGACTTTGTACAAAAGGGTTATACACTGAAGTGTCAAACACAGTCAGATTTTGGGAAAGTGAC
  AATGCAATTTGAATTAGAAGTGTGCCAGCTTCAAAAACCCGATGTGGTGGGTATCAGGAGGCAGCGGCTT
  AAGGGCGATGCCTGGGTTTACAAAAGATTAGTGGAAGACATCCTATCTAGCTGCAAGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232990 representing NM_001256688
  Red=Cloning site Green=Tags(s)
  
  MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNLRHQHIC
  QLYHVLETANKIFMVLEGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDD
  DNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKKRISMKNLLNHPWIMQDYNYPVEWQSKNPFIH
  LDDDCVTELSVHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGKPVRLRLSSFSCGQASATPFTDIK
  SNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWTESNGVESKSLTPALCRTPANKLK
  NKENVYTPKSAVKNEEYFMFPEPKTPVNKNQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTDK
  LMTGVISPERRCRSVELDLNQAHMEETPKRKGAKVFGSLERGLDKVITVLTRSKRKGSARDGPRRLKLHY
  NVTTTRLVNPDQLLNEIMSILPKKHVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQKPDVVGIRRQRL
  KGDAWVYKRLVEDILSSCKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256688
• ORF Size: 1740 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256688.1, NP_001243617.1
• RefSeq Size: 2273 bp
• RefSeq ORF: 1743 bp
• Locus ID: 9833
• UniProt ID: Q14680
• Cytogenetics: 9p13.2
• Protein Families: Druggable Genome, Protein Kinase
• MW: 66.8 kDa
• Gene Summary: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.[UniProtKB/Swiss-Prot Function]