COL11A1 (NM_001190709) Human Tagged ORF Clone

CAT#: RC231490

COL11A1 (Myc-DDK-tagged)-Human collagen, type XI, alpha 1 (COL11A1), transcript variant E

Product Images

Circular map for RC231490

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COL11A1
• Synonyms: CO11A1; COLL6; DFNA37; STL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231490 representing NM_001190709
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGTGGTCCTCTAGGTGGAAAACGAAACGGTGGCTCTGGGATTTCACCGTAACAACCCTCGCAT
  TGACCTTCCTCTTCCAAGCTAGAGAGGTCAGAGGAGCTGCTCCAGTTGATGTACTAAAAGCACTAGATTT
  TCACAATTCTCCAGAGGGAATATCAAAAACAACGGGATTTTGCACAAACAGAAAGAATTCTAAAGGCTCA
  GATACTGCTTACAGAGTTTCAAAGCAAGCACAACTCAGTGCCCCAACAAAACAGTTATTTCCAGGTGGAA
  CTTTCCCAGAAGACTTTTCAATACTATTTACAGTAAAACCAAAAAAAGGAATTCAGTCTTTCCTTTTATC
  TATATATAATGAGCATGGTATTCAGCAAATTGGTGTTGAGGTTGGGAGATCACCTGTTTTTCTGTTTGAA
  GACCACACTGGAAAACCTGCCCCAGAAGACTATCCCCTCTTCAGAACTGTTAACATCGCTGACGGGAAGT
  GGCATCGGGTAGCAATCAGCGTGGAGAAGAAAACTGTGACAATGATTGTTGATTGTAAGAAGAAAACCAC
  GAAACCACTTGATAGAAGTGAGAGAGCAATTGTTGATACCAATGGAATCACGGTTTTTGGAACAAGGATT
  TTGGATGAAGAAGTTTTTGAGGGGGACATTCAGCAGTTTTTGATCACAGGTGATCCCAAGGCAGCATATG
  ACTACTGTGAGCATTATAGTCCAGACTGTGACTCTTCAGCACCCAAGGCTGCTCAAGCTCAGGAACCTCA
  GATAGATGAGGCAAACATCGTTGATGATTTTCAAGAATACAACTATGGAACAATGGAAAGTTACCAGACA
  GAAGCTCCTAGGCATGTTTCTGGGACAAATGAGCCAAATCCAGTTGAAGAAATATTTACTGAAGAATATC
  TAACGGGAGAGGATTATGATTCCCAGAGGAAAAATTCTGAGGATACACTATATGAAAACAAAGAAATAGA
  CGGCAGGGATTCTGATCTTCTGGTAGATGGAGATTTAGGCGAATATGATTTTTATGAATATAAAGAATAT
  GAAGATAAACCAACAAGCCCCCCTAATGAAGAATTTGGTCCAGGTGTACCAGCAGAAACTGATATTACAG
  AAACAAGCATAAATGGCCATGGTGCATATGGAGAGAAAGGACAGAAAGGAGAACCAGCAGTGGTTGAGCC
  TGGTATGCTTGTCGAAGGACCACCAGGACCAGCAGGACCTGCAGGTATTATGGGTCCTCCAGGTCTACAA
  GGCCCCACTGGACCCCCTGGTGACCCTGGCGATAGGGGCCCCCCAGGACGTCCTGGCTTACCAGGGGCTG
  ATGGTCTACCTGGTCCTCCTGGTACTATGTTGATGTTACCGTTCCGTTATGGTGGTGATGGTTCCAAAGG
  ACCAACCATCTCTGCTCAGGAAGCTCAGGCTCAAGCTATTCTTCAGCAGGCTCGGATTGCTCTGAGAGGC
  CCACCTGGCCCAATGGGTCTAACTGGAAGACCAGGTCCTGTGGGGGGGCCTGGTTCATCTGGGGCCAAAG
  GTGAGAGTGGTGATCCAGGTCCTCAGGGCCCTCGAGGCGTCCAGGGTCCCCCTGGTCCAACGGGAAAACC
  TGGAAAAAGGGGTCGTCCAGGTGCAGATGGAGGAAGAGGAATGCCAGGAGAACCTGGGGCAAAGGGAGAT
  CGAGGGTTTGATGGACTTCCGGGTCTGCCAGGTGACAAAGGTCACAGGGGTGAACGAGGTCCTCAAGGTC
  CTCCAGGTCCTCCTGGTGATGATGGAATGAGGGGAGAAGATGGAGAAATTGGACCAAGAGGTCTTCCAGG
  TGAAGCTGGCCCACGAGGTTTGCTGGGTCCAAGGGGAACTCCAGGAGCTCCAGGGCAGCCTGGTATGGCA
  GGTGTAGATGGCCCCCCAGGACCAAAAGGGAACATGGGTCCCCAAGGGGAGCCTGGGCCTCCAGGTCAAC
  AAGGGAATCCAGGACCTCAGGGTCTTCCTGGTCCACAAGGTCCAATTGGTCCTCCTGGTGAAAAAGGACC
  ACAAGGAAAACCAGGACTTGCTGGACTTCCTGGTGCTGATGGGCCTCCTGGTCATCCTGGGAAAGAAGGC
  CAGTCTGGAGAAAAGGGGGCTCTGGGTCCCCCTGGTCCACAAGGTCCTATTGGATACCCGGGCCCCCGGG
  GAGTAAAGGGAGCAGATGGTGTCAGAGGTCTCAAGGGATCTAAAGGTGAAAAGGGTGAAGATGGTTTTCC
  AGGATTCAAAGGTGACATGGGTCTAAAAGGTGACAGAGGAGAAGTTGGTCAAATTGGCCCAAGAGGGGAA
  GATGGCCCTGAAGGACCCAAAGGTCGAGCAGGCCCAACTGGAGACCCAGGTCCTTCAGGTCAAGCAGGAG
  AAAAGGGAAAACTTGGAGTTCCAGGATTACCAGGATATCCAGGAAGACAAGGTCCAAAGGGTTCCACTGG
  ATTCCCTGGGTTTCCAGGTGCCAATGGAGAGAAAGGTGCACGGGGAGTAGCTGGCAAACCAGGCCCTCGG
  GGTCAGCGTGGTCCAACGGGTCCTCGAGGTTCAAGAGGTGCAAGAGGTCCCACTGGGAAACCTGGGCCAA
  AGGGCACTTCAGGTGGCGATGGCCCTCCTGGCCCTCCAGGTGAAAGAGGTCCTCAAGGACCTCAGGGTCC
  AGTTGGATTCCCTGGACCAAAAGGCCCTCCTGGACCACCTGGGAAGGATGGGCTGCCAGGACACCCTGGG
  CAACGTGGGGAGACTGGATTTCAAGGCAAGACCGGCCCTCCTGGGCCAGGGGGAGTGGTTGGACCACAGG
  GACCAACCGGTGAGACTGGTCCAATAGGGGAACGTGGGCATCCTGGCCCTCCTGGCCCTCCTGGTGAGCA
  AGGTCTTCCTGGTGCTGCAGGAAAAGAAGGTGCAAAGGGTGATCCAGGTCCTCAAGGTATCTCAGGGAAA
  GATGGACCAGCAGGATTACGTGGTTTCCCAGGGGAAAGAGGTCTTCCTGGAGCTCAGGGTGCACCTGGAC
  TGAAAGGAGGGGAAGGTCCCCAGGGCCCACCAGGTCCAGTTGGCTCACCAGGAGAACGTGGGTCAGCAGG
  TACAGCTGGCCCAATTGGTTTACCAGGGCGCCCGGGACCTCAGGGTCCTCCTGGTCCAGCTGGAGAGAAA
  GGTGCTCCTGGAGAAAAAGGTCCCCAAGGGCCTGCAGGGAGAGATGGAGTTCAAGGTCCTGTTGGTCTCC
  CAGGGCCAGCTGGTCCTGCCGGCTCCCCTGGGGAAGACGGAGACAAGGGTGAAATTGGTGAGCCGGGACA
  AAAAGGCAGCAAGGGTGACAAGGGAGAAAATGGCCCTCCCGGTCCCCCAGGTCTTCAAGGACCAGTTGGT
  GCCCCTGGAATTGCTGGAGGTGATGGTGAACCAGGTCCTAGAGGACAGCAGGGGATGTTTGGGCAAAAAG
  GTGATGAGGGTGCCAGAGGCTTCCCTGGACCTCCTGGTCCAATAGGTCTTCAGGGTCTGCCAGGCCCACC
  TGGTGAAAAAGGTGAAAATGGGGATGTTGGTCCCATGGGGCCACCTGGTCCTCCAGGCCCAAGAGGCCCT
  CAAGGTCCCAATGGAGCTGATGGACCACAAGGACCCCCAGGGTCTGTTGGTTCAGTTGGTGGTGTTGGAG
  AAAAGGGTGAACCTGGAGAAGCAGGGAACCCAGGGCCTCCTGGGGAAGCAGGTGTAGGCGGTCCCAAAGG
  AGAAAGAGGAGAGAAAGGGGAAGCTGGTCCACCTGGAGCTGCTGGACCTCCAGGTGCCAAGGGGCCACCA
  GGTGATGATGGCCCTAAGGGTAACCCGGGTCCTGTTGGTTTTCCTGGAGATCCTGGTCCTCCTGGGGAAC
  CTGGCCCTGCAGGTCAAGATGGTGTTGGTGGTGACAAGGGTGAAGATGGAGATCCTGGTCAACCGGGTCC
  TCCTGGCCCATCTGGTGAGGCTGGCCCACCAGGTCCTCCTGGAAAACGAGGTCCTCCTGGAGCTGCAGGT
  GCAGAGGGAAGACAAGGTGAAAAAGGTGCTAAGGGGGAAGCAGGTGCAGAAGGTCCTCCTGGAAAAACCG
  GCCCAGTCGGTCCTCAGGGACCTGCAGGAAAGCCTGGTCCAGAAGGTCTTCGGGGCATCCCTGGTCCTGT
  GGGAGAACAAGGTCTCCCTGGAGCTGCAGGCCAAGATGGACCACCTGGTCCTATGGGACCTCCTGGCTTA
  CCTGGTCTCAAAGGTGACCCTGGCTCCAAGGGTGAAAAGGGACATCCTGGTTTAATTGGCCTGATTGGTC
  CTCCAGGAGAACAAGGGGAAAAAGGTGACCGAGGGCTCCCTGGAACTCAAGGATCTCCAGGAGCAAAAGG
  GGATGGGGGAATTCCTGGTCCTGCTGGTCCCTTAGGTCCACCTGGTCCTCCAGGTTTACCAGGTCCTCAA
  GGCCCAAAGGGTAACAAAGGCTCTACTGGACCCGCTGGCCAGAAAGGTGACAGTGGTCTTCCAGGGCCTC
  CTGGGTCTCCAGGTCCACCTGGTGAAGTCATTCAGCCTTTACCAATCTTGTCCTCCAAAAAAACGAGAAG
  ACATACTGAAGGCATGCAAGCAGATGCAGATGATAATATTCTTGATTACTCGGATGGAATGGAAGAAATA
  TTTGGTTCCCTCAATTCCCTGAAACAAGACATTGAGCATATGAAATTTCCAATGGGTACTCAGACCAATC
  CAGCCCGAACTTGTAAAGACCTGCAACTCAGCCATCCTGACTTCCCAGATGGTGAATATTGGATTGATCC
  TAACCAAGGTTGCTCAGGAGATTCCTTCAAAGTTTACTGTAATTTCACATCTGGTGGTGAGACTTGCATT
  TATCCAGACAAAAAATCTGAGGGAGTAAGAATTTCATCATGGCCAAAGGAGAAACCAGGAAGTTGGTTTA
  GTGAATTTAAGAGGGGAAAACTGCTTTCATACTTAGATGTTGAAGGAAATTCCATCAATATGGTGCAAAT
  GACATTCCTGAAACTTCTGACTGCCTCTGCTCGGCAAAATTTCACCTACCACTGTCATCAGTCAGCAGCC
  TGGTATGATGTGTCATCAGGAAGTTATGACAAAGCACTTCGCTTCCTGGGATCAAATGATGAGGAGATGT
  CCTATGACAATAATCCTTTTATCAAAACACTGTATGATGGTTGTGCGTCCAGAAAAGGCTATGAAAAGAC
  TGTCATTGAAATCAATACACCAAAAATTGATCAAGTACCTATTGTTGATGTCATGATCAATGACTTTGGT
  GATCAGAATCAGAAGTTCGGATTTGAAGTTGGTCCTGTTTGTTTTCTTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231490 representing NM_001190709
  Red=Cloning site Green=Tags(s)
  
  MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNSPEGISKTTGFCTNRKNSKGS
  DTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFE
  DHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDTNGITVFGTRI
  LDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKAAQAQEPQIDEANIVDDFQEYNYGTMESYQT
  EAPRHVSGTNEPNPVEEIFTEEYLTGEDYDSQRKNSEDTLYENKEIDGRDSDLLVDGDLGEYDFYEYKEY
  EDKPTSPPNEEFGPGVPAETDITETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPAGIMGPPGLQ
  GPTGPPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRG
  PPGPMGLTGRPGPVGGPGSSGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGD
  RGFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGAPGQPGMA
  GVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEG
  QSGEKGALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGE
  DGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPR
  GQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPG
  QRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAAGKEGAKGDPGPQGISGK
  DGPAGLRGFPGERGLPGAQGAPGLKGGEGPQGPPGPVGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEK
  GAPGEKGPQGPAGRDGVQGPVGLPGPAGPAGSPGEDGDKGEIGEPGQKGSKGDKGENGPPGPPGLQGPVG
  APGIAGGDGEPGPRGQQGMFGQKGDEGARGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGP
  QGPNGADGPQGPPGSVGSVGGVGEKGEPGEAGNPGPPGEAGVGGPKGERGEKGEAGPPGAAGPPGAKGPP
  GDDGPKGNPGPVGFPGDPGPPGEPGPAGQDGVGGDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGAAG
  AEGRQGEKGAKGEAGAEGPPGKTGPVGPQGPAGKPGPEGLRGIPGPVGEQGLPGAAGQDGPPGPMGPPGL
  PGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGTQGSPGAKGDGGIPGPAGPLGPPGPPGLPGPQ
  GPKGNKGSTGPAGQKGDSGLPGPPGSPGPPGEVIQPLPILSSKKTRRHTEGMQADADDNILDYSDGMEEI
  FGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCI
  YPDKKSEGVRISSWPKEKPGSWFSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAA
  WYDVSSGSYDKALRFLGSNDEEMSYDNNPFIKTLYDGCASRKGYEKTVIEINTPKIDQVPIVDVMINDFG
  DQNQKFGFEVGPVCFLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190709
• ORF Size: 5301 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190709.1, NP_001177638.1
• RefSeq ORF: 5304 bp
• Locus ID: 1301
• UniProt ID: P12107
• Cytogenetics: 1p21.1
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• MW: 177.1 kDa
• Gene Summary: This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. A single-nucleotide polymorphism in this gene is also associated with susceptibility to lumbar disc herniation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]