ABCC10 (NM_001198934) Human Tagged ORF Clone

CAT#: RC231486

ABCC10 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 10 (ABCC10), transcript variant MRP7

Product Images

Circular map for RC231486

• 

  Western blot validation of overexpression lysate (Cat# LY434484) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231486 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCC10
• Synonyms: EST182763; MRP7; SIMRP7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231486 representing NM_001198934
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACGACTTCTGGCCCAGCTGTGCGGCAGCAGCGCAGCGTGGCCGCTCCCGCTGTGGGAGGGGGACA
  CCACAGGCCACTGCTTCACCCAGCTGGTGCTCAGCGCCCTGCCCCACGCGCTCCTCGCCGTGCTCAGTGC
  CTGTTACTTGGGCACCCCGAGGAGTCCAGATTACATCCTACCCTGCAGTCCTGGATGGCGCCTCCGACTT
  GCAGCTTCCTTCCTGCTTTCCGTCTTCCCGCTGCTAGACCTTCTTCCAGTTGCTTTGCCACCAGGGGCAG
  GCCCAGGACCCATAGGGCTAGAGGTGTTGGCAGGGTGCGTGGCAGCTGTGGCCTGGATCAGCCACAGCCT
  GGCCCTGTGGGTGTTGGCACATTCCCCTCATGGCCACTCCCGGGGTCCCTTGGCCTTGGCCCTGGTAGCC
  TTGCTGCCAGCTCCAGCCCTAGTGCTGACCGTGTTGTGGCATTGCCAGCGAGGCACACTTCTGCCCCCAC
  TTCTCCCAGGGCCCATGGCCCGCCTATGCTTGCTCATCCTGCAGCTGGCTGCACTCTTGGCCTATGCACT
  GGGATGGGCAGCTCCTGGGGGACCACGAGAACCCTGGGCTCAGGAGCCCCTCCTGCCCGAGGATCAAGAA
  CCTGAGGTGGCTGAAGATGGGGAGAGTTGGCTGTCACGCTTTTCCTATGCCTGGCTGGCACCCTTGCTGG
  CCCGTGGGGCCTGTGGAGAGCTCCGGCAGCCTCAGGACATTTGCCGCCTCCCCCACAGACTGCAGCCAAC
  CTACCTGGCTCGTGTCTTCCAGGCACACTGGCAGGAGGGGGCACGGCTGTGGAGGGCCTTGTATGGGGCC
  TTTGGACGGTGCTATCTGGCACTTGGACTGCTGAAGCTGGTGGGGACCATGTTGGGATTCTCAGGGCCCC
  TGTTGCTCTCCCTACTGGTGGGCTTCCTGGAAGAGGGGCAGGAGCCACTAAGCCACGGCCTGCTCTATGC
  TCTGGGGCTAGCCGGTGGGGCTGTGCTGGGTGCTGTGCTGCAGAATCAGTATGGGTATGAGGTATATAAG
  GTAACACTTCAGGCACGGGGGGCTGTGCTGAACATCCTGTACTGCAAGGCTTTACAGCTGGGGCCCAGCC
  GCCCTCCTACTGGGGAGGCCCTGAACCTACTAGGCACTGACTCTGAACGGCTGCTTAACTTTGCTGGGAG
  CTTCCATGAAGCCTGGGGCCTGCCCCTGCAACTGGCCATCACCCTCTACCTGCTGTACCAGCAGGTAGGC
  GTGGCCTTCGTGGGTGGTCTCATCTTGGCACTGCTGCTGGTACCCGTCAACAAAGTGATTGCCACCCGCA
  TCATGGCCAGCAACCAGGAAATGCTACAGCACAAGGATGCGCGGGTTAAGCTTGTGACAGAGCTGCTGAG
  TGGCATTCGGGTCATCAAGTTCTGCGGGTGGGAGCAGGCACTGGGAGCCCGAGTAGAGGCCTGCCGGGCT
  CGAGAGCTGGGGCGACTCCGGGTCATCAAATACCTGGATGCGGCCTGTGTATACCTGTGGGCTGCCCTAC
  CGGTTGTCATCTCCATCGTTATCTTCATCACCTATGTCCTCATGGGGCACCAGCTCACTGCCACCAAGGT
  GTTCACGGCCCTGGCACTGGTGCGAATGCTCATTCTTCCTCTCAACAACTTCCCTTGGGTGATCAATGGT
  CTCCTGGAGGCCAAAGTGTCCTTGGACCGGATCCAGCTTTTCCTCGACCTTCCAAACCACAACCCCCAGG
  CCTACTACAGCCCAGATCCCCCTGCAGAGCCATCTACAGTATTGGAGCTGCATGGAGCCTTGTTCTCCTG
  GGACCCAGTTGGAACCAGCCTGGAGACCTTCATCAGTCATCTCGAAGTGAAAAAGGGTATGCTGGTGGGC
  ATCGTGGGGAAGGTCGGCTGTGGGAAGAGCTCCCTGCTGGCTGCCATCGCTGGAGAGCTCCACAGGCTGC
  GTGGGCATGTGGCAGTGCGGGGGCTGTCCAAGGGCTTTGGCCTGGCCACCCAGGAACCCTGGATCCAGTT
  TGCCACCATCCGAGACAACATCCTCTTTGGGAAGACATTTGATGCACAGCTGTACAAGGAGGTGCTAGAA
  GCCTGCGCCCTCAATGATGACCTCAGTATCCTGCCTGCTGGAGACCAGACAGAGGTGGGGGAGAAGGGTG
  TCACCCTTAGCGGAGGACAGCGTGCCCGGATTGCCCTTGCTCGTGCTGTCTACCAGGAAAAGGAGCTCTA
  TCTCCTCGATGACCCTCTGGCCGCTGTGGATGCAGATGTGGCCAACCACCTGCTGCACAGGTGCATCCTG
  GGCATGCTGAGCTACACCACACGGCTGCTCTGCACCCACCGCACTGAGTACCTGGAGAGGGCTGACGCGG
  TGCTGCTGATGGAGGCCGGGCGCCTCATCCGGGCTGGACCTCCCTCTGAGATTCTGCCACTGGTACAAGC
  TGTCCCCAAAGCCTGGGCTGAGAATGGACAAGAGTCTGACTCAGCCACAGCCCAGTCAGTACAGAACCCA
  GAGAAAACAAAGGAGGGGCTGGAGGAGGAGCAGAGCACATCTGGTCGCCTGCTGCAGGAAGAAAGCAAGA
  AGGAGGGCGCCGTGGCCTTGCACGTGTACCAAGCTTACTGGAAGGCCGTGGGCCAGGGCTTGGCCTTAGC
  CATCCTCTTCTCTCTGCTTCTCATGCAAGCCACGCGGAACGCTGCTGACTGGTGGCTCTCCCACTGGATC
  TCTCAGCTGAAGGCTGAGAATAGCTCCCAGGAGGCGCAACCCTCCACCAGCCCAGCTTCTATGGGGCTCT
  TCTCTCCGCAGCTGCTCCTCTTTTCCCCTGGAAACCTCTACATCCCAGTGTTCCCACTGCCCAAAGCTGC
  CCCCAATGGCTCCTCAGACATCCGTTTCTACCTCACCGTGTATGCGACCATTGCTGGTGTAAATTCCCTC
  TGCACCCTTCTCCGGGCAGTGCTCTTTGCAGCAGGCACCCTTCAAGCAGCTGCCACTCTGCATCGCCGCC
  TGCTGCATCGAGTCCTTATGGCACCAGTGACTTTCTTCAATGCCACACCCACGGGCCGGATCCTAAACCG
  CTTCTCCTCTGATGTGGCCTGTGCGGATGACAGCCTGCCCTTCATCCTCAACATCCTCCTGGCCAACGCG
  GCAGGCCTGCTGGGGCTCCTGGCCGTGCTGGGCTCTGGCCTGCCCTGGCTGCTGCTCCTGCTGCCGCCTT
  TGAGCATCATGTACTATCACGTGCAGCGCCACTACAGGGCCTCCTCACGGGAGCTGCGGCGCCTGGGCAG
  CCTCACCCTGTCTCCACTGTATAGCCATCTGGCCGATACCTTGGCTGGCCTCTCTGTGCTCCGGGCCACA
  GGGGCCACCTACAGGTTTGAGGAGGAGAACCTGCGACTCCTTGAGCTAAACCAGAGGTGCCAGTTTGCCA
  CCAGTGCCACAATGCAGTGGCTGGACATTCGGCTACAGCTCATGGGGGCGGCAGTGGTCAGCGCTATCGC
  AGGCATCGCTCTGGTGCAGCACCAGCAGGGCCTCGCTAACCCAGGGCTGGTGGGCTTGTCGCTGTCTTAT
  GCCCTGTCCCTGACGGGCCTGCTCTCGGGCCTGGTGAGCAGCTTCACACAGACAGAGGCCATGCTGGTGA
  GCGTCGAGCGGCTGGAAGAGTACACCTGTGACCTGCCCCAGGAACCCCAGGGCCAGCCACTGCAGCTGGG
  CACCGGCTGGCTGACCCAGGGGGGCGTGGAGTTCCAGGACGTGGTGTTGGCGTACCGGCCAGGGCTGCCG
  AATGCCCTGGATGGAGTGACCTTCTGCGTGCAGCCTGGAGAGAAGTTGGGCATCGTGGGCCGCACAGGCT
  CCGGCAAGTCTTCCCTGTTGTTGGTGCTCTTCCGGCTGCTAGAGCCCAGTTCAGGGCGAGTGCTGCTGGA
  CGGCGTGGACACCAGCCAGCTGGAGCTGGCCCAGCTCAGATCCCAGTTGGCTATCATCCCCCAGGAGCCC
  TTTTTGTTCAGTGGGACTGTTCGGGAAAACCTGGACCCCCAGGGCCTACATAAGGACAGGGCCTTGTGGC
  AGGCCCTGAAGCAGTGCCACCTGAGTGAGGTGATTACATCCATGGGTGGTCTGGATGGTGAGCTGGGTGA
  GGGGGGCCGGAGCTTATCTCTTGGGCAGAGGCAGCTGTTGTGTTTGGCCAGGGCTCTCCTCACAGATGCC
  AAGATCCTGTGTATCGATGAGGCCACAGCAAGTGTGGACCAGAAGACAGACCAGCTGCTCCAGCAGACCA
  TCTGCAAACGCTTTGCCAACAAGACAGTGCTGACCATTGCCCATAGGCTCAACACGATCCTGAACTCAGA
  CCGGGTGCTGGTGCTACAAGCGGGGAGAGTGGTAGAGCTGGACTCCCCGGCCACCCTGCGCAACCAGCCC
  CACTCCCTGTTCCAGCAGCTGCTGCAGAGCAGCCAGCAGGGAGTCCCTGCCTCACTCGGAGGTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231486 representing NM_001198934
  Red=Cloning site Green=Tags(s)
  
  MERLLAQLCGSSAAWPLPLWEGDTTGHCFTQLVLSALPHALLAVLSACYLGTPRSPDYILPCSPGWRLRL
  AASFLLSVFPLLDLLPVALPPGAGPGPIGLEVLAGCVAAVAWISHSLALWVLAHSPHGHSRGPLALALVA
  LLPAPALVLTVLWHCQRGTLLPPLLPGPMARLCLLILQLAALLAYALGWAAPGGPREPWAQEPLLPEDQE
  PEVAEDGESWLSRFSYAWLAPLLARGACGELRQPQDICRLPHRLQPTYLARVFQAHWQEGARLWRALYGA
  FGRCYLALGLLKLVGTMLGFSGPLLLSLLVGFLEEGQEPLSHGLLYALGLAGGAVLGAVLQNQYGYEVYK
  VTLQARGAVLNILYCKALQLGPSRPPTGEALNLLGTDSERLLNFAGSFHEAWGLPLQLAITLYLLYQQVG
  VAFVGGLILALLLVPVNKVIATRIMASNQEMLQHKDARVKLVTELLSGIRVIKFCGWEQALGARVEACRA
  RELGRLRVIKYLDAACVYLWAALPVVISIVIFITYVLMGHQLTATKVFTALALVRMLILPLNNFPWVING
  LLEAKVSLDRIQLFLDLPNHNPQAYYSPDPPAEPSTVLELHGALFSWDPVGTSLETFISHLEVKKGMLVG
  IVGKVGCGKSSLLAAIAGELHRLRGHVAVRGLSKGFGLATQEPWIQFATIRDNILFGKTFDAQLYKEVLE
  ACALNDDLSILPAGDQTEVGEKGVTLSGGQRARIALARAVYQEKELYLLDDPLAAVDADVANHLLHRCIL
  GMLSYTTRLLCTHRTEYLERADAVLLMEAGRLIRAGPPSEILPLVQAVPKAWAENGQESDSATAQSVQNP
  EKTKEGLEEEQSTSGRLLQEESKKEGAVALHVYQAYWKAVGQGLALAILFSLLLMQATRNAADWWLSHWI
  SQLKAENSSQEAQPSTSPASMGLFSPQLLLFSPGNLYIPVFPLPKAAPNGSSDIRFYLTVYATIAGVNSL
  CTLLRAVLFAAGTLQAAATLHRRLLHRVLMAPVTFFNATPTGRILNRFSSDVACADDSLPFILNILLANA
  AGLLGLLAVLGSGLPWLLLLLPPLSIMYYHVQRHYRASSRELRRLGSLTLSPLYSHLADTLAGLSVLRAT
  GATYRFEEENLRLLELNQRCQFATSATMQWLDIRLQLMGAAVVSAIAGIALVQHQQGLANPGLVGLSLSY
  ALSLTGLLSGLVSSFTQTEAMLVSVERLEEYTCDLPQEPQGQPLQLGTGWLTQGGVEFQDVVLAYRPGLP
  NALDGVTFCVQPGEKLGIVGRTGSGKSSLLLVLFRLLEPSSGRVLLDGVDTSQLELAQLRSQLAIIPQEP
  FLFSGTVRENLDPQGLHKDRALWQALKQCHLSEVITSMGGLDGELGEGGRSLSLGQRQLLCLARALLTDA
  KILCIDEATASVDQKTDQLLQQTICKRFANKTVLTIAHRLNTILNSDRVLVLQAGRVVELDSPATLRNQP
  HSLFQQLLQSSQQGVPASLGGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198934
• ORF Size: 4476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198934.2
• RefSeq ORF: 4479 bp
• Locus ID: 89845
• UniProt ID: Q5T3U5
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 162.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This ABC full-transporter is a member of the MRP subfamily which is involved in multi-drug resistance. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2010]