Ensconsin (MAP7) (NM_001198616) Human Tagged ORF Clone

CAT#: RC231388

MAP7 (Myc-DDK-tagged)-Human microtubule-associated protein 7 (MAP7), transcript variant 7

Product Images

Circular map for RC231388

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ensconsin
• Synonyms: E-MAP-115; EMAP115
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231388 representing NM_001198616
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTAGGAGCTGGCGGCGACGGCCACAGGGGCGGCGACGGCGCAGTGCGAAGCGAAACAGCAC
  CCGACAGCTACAAAGTGCAAGATAAGAAAAATGCCTCCAGCCGCCCTGCCTCTGCAATTTCAGGACAAAA
  TAACAACCACTCAGGAAATAAACCAGACCCTCCGCCTGTGTTACGTGTTGATGACCGGCAGCGGCTGGCC
  CGGGAGCGACGTGAGGAACGGGAGAAACAGCTAGCTGCAAGAGAAATAGTGTGGTTAGAAAGAGAAGAGC
  GAGCCAGGCAGCACTACGAGAAGCACCTGGAAGAGCGGAAGAAGAGGTTGGAGGAGCAGAGGCAGAAGGA
  GGAGCGGAGGAGGGCTGCTGTGGAGGAGAAGCGGAGGCAGAGACTTGAGGAGGACAAAGAACGCCACGAA
  GCTGTTGTACGGCGCACAATGGAAAGGAGCCAGAAGCCAAAACAGAAGCATAACCGTTGGTCGTGGGGAG
  GCTCTCTCCATGGGAGCCCTAGCATCCACAGTGCAGCTCGCCGCCTGCAGCTCAGCCCATGGGAGAGCAG
  CGTTGTTAACAGACTCCTGACGCCCACACATTCGTTCCTGGCCAGAAGTAAAAGCACAGCTGCCTTGTCT
  GGAGAAGCAGCATCTTGCAGCCCCATCATCATGCCCTACAAAGCTGCACACTCTAGAAATTCGATGGATC
  GACCAAAACTCTTTGTAACACCACCTGAGGGCTCTTCTCGCAGGAGGATCATTCATGGCACAGCGAGCTA
  TAAAAAAGAAAGAGAGAGAGAAAATGTACTCTTCCTCACATCTGGCACCCGAAGGGCTGTATCTCCATCT
  AATCCCAAAGCAAGACAACCAGCTCGCTCCCGACTTTGGCTTCCGTCCAAGTCTCTTCCTCATTTGCCTG
  GCACACCCAGACCGACATCCTCCTTGCCACCCGGCTCAGTCAAAGCTGCTCCTGCTCAGGTCCGGCCCCC
  ATCCCCCGGCAACATCCGCCCTGTCAAGAGGGAAGTCAAAGTGGAGCCTGAGAAGAAAGATCCTGAGAAG
  GAACCTCAGAAAGTTGCCAATGAGCCCTCACTAAAGGGCAGAGCACCTTTAGTGAAGGTAGAAGAAGCCA
  CAGTTGAAGAGCGGACACCTGCTGAACCAGAAGTTGGCCCTGCTGCTCCAGCCATGGCCCCAGCTCCAGC
  CTCGGCCCCAGCTCCAGCCTCGGCCCCAGCTCCAGCCCCGGTCCCCACCCCAGCCATGGTCTCAGCCCCG
  TCATCCACTGTGAATGCCAGTGCTTCTGTTAAGACTTCTGCAGGCACCACCGACCCAGAGGAGGCCACAA
  GGCTTCTAGCTGAGAAGAGGCGGCTGGCCCGAGAGCAGAGAGAAAAGGAAGAAAGGGAGAGGAGGGAGCA
  GGAAGAGCTTGAAAGACAAAAGAGAGAGGAATTGGCTCAACGTGTGGCTGAAGAGAGGACGACTCGCCGT
  GAGGAGGAGTCGCGCAGGCTGGAAGCCGAGCAGGCCCGGGAGAAGGAGGAGCAGCTGCAGCGGCAGGCGG
  AGGAGCGGGCGCTGCGCGAGCGGGAGGAGGCAGAGCGCGCCCAGAGGCAGAAAGAAGAAGAAGCTCGCGT
  TCGTGAAGAAGCAGAGAGGGTCCGGCAGGAACGAGAGAAGCATTTCCAGAGAGAAGAGCAAGAGCGCCTG
  GAGAGAAAGAAGCGACTTGAGGAGATTATGAAAAGAACCAGGAGAACAGAAGCTACAGATAAGAAAACCA
  GTGATCAGAGAAACGGTGATATAGCCAAGGGAGCTCTCACTGGAGGAACAGAGGTGTCTGCACTTCCATG
  TACAACAAACGCTCCGGGAAATGGAAAGCCAGTTGGCAGCCCACATGTGGTTACCTCACACCAGTCAAAA
  GTGACAGTGGAGAGCACTCCCGATTTGGAAAAACAACCAAATGAAAATGGTGTATCTGTTCAGAATGAAA
  ATTTTGAAGAAATTATAAACTTACCCATTGGATCTAAACCATCCAGATTAGATGTCACCAACAGTGAGAG
  CCCAGAAATTCCTTTGAATCCAATTTTGGCCTTTGATGATGAAGGGACACTTGGGCCCCTGCCTCAGGTA
  GATGGTGTTCAGACACAGCAGACTGCAGAAGTTATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231388 representing NM_001198616
  Red=Cloning site Green=Tags(s)
  
  MAELGAGGDGHRGGDGAVRSETAPDSYKVQDKKNASSRPASAISGQNNNHSGNKPDPPPVLRVDDRQRLA
  RERREEREKQLAAREIVWLEREERARQHYEKHLEERKKRLEEQRQKEERRRAAVEEKRRQRLEEDKERHE
  AVVRRTMERSQKPKQKHNRWSWGGSLHGSPSIHSAARRLQLSPWESSVVNRLLTPTHSFLARSKSTAALS
  GEAASCSPIIMPYKAAHSRNSMDRPKLFVTPPEGSSRRRIIHGTASYKKERERENVLFLTSGTRRAVSPS
  NPKARQPARSRLWLPSKSLPHLPGTPRPTSSLPPGSVKAAPAQVRPPSPGNIRPVKREVKVEPEKKDPEK
  EPQKVANEPSLKGRAPLVKVEEATVEERTPAEPEVGPAAPAMAPAPASAPAPASAPAPAPVPTPAMVSAP
  SSTVNASASVKTSAGTTDPEEATRLLAEKRRLAREQREKEERERREQEELERQKREELAQRVAEERTTRR
  EEESRRLEAEQAREKEEQLQRQAEERALREREEAERAQRQKEEEARVREEAERVRQEREKHFQREEQERL
  ERKKRLEEIMKRTRRTEATDKKTSDQRNGDIAKGALTGGTEVSALPCTTNAPGNGKPVGSPHVVTSHQSK
  VTVESTPDLEKQPNENGVSVQNENFEEIINLPIGSKPSRLDVTNSESPEIPLNPILAFDDEGTLGPLPQV
  DGVQTQQTAEVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198616
• ORF Size: 2136 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198616.1, NP_001185545.1
• RefSeq ORF: 2139 bp
• Locus ID: 9053
• UniProt ID: Q14244
• Cytogenetics: 6q23.3
• Protein Families: Druggable Genome
• MW: 80.4 kDa
• Gene Summary: The product of this gene is a microtubule-associated protein that is predominantly expressed in cells of epithelial origin. Microtubule-associated proteins are thought to be involved in microtubule dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtubules, and may serve to modulate microtubule functions. Studies of the related mouse protein also suggested an essential role in microtubule function required for spermatogenesis. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]