CRMP2 (DPYSL2) (NM_001197293) Human Tagged ORF Clone

CAT#: RC231368

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DPYSL2 (Myc-DDK-tagged)-Human dihydropyrimidinase-like 2 (DPYSL2), transcript variant 1



  "NM_001197293" in other vectors (5)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 862.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DPYSL2
Synonyms CRMP-2; CRMP2; DHPRP2; DRP-2; DRP2; N2A3; ULIP-2; ULIP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC231368 representing NM_001197293
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGAGAAAGCAATCCGGGAAGGCGGCAGAGGACGAAGAGGTCCCTGCTTTTTTTAAAAACCTGG
GCTCCGGCAGCCCCAAGCCCCGGCAGAAATTCTGTGGCATGTTCTGCCCGGTGGAAGGGTCCTCGGAGAA
CAAGACCATCGACTTCGACTCGCTGTCGGTGGGCCGGGGCTCGGGGCAGGTGGTGGCTCAGCAGCGGGAC
GTCGCCCACTTGGGCCCGGACCCGCAGCCGCCGTACTCGCGGCAGGGCCGGCGCGCCGGCGGAGAGCCAT
CTGTTGAATCGGGCCGGAAGGTGGAGATCCGGAGGGCCTCGGGCAAAGAAGCCCTGCAGAACATCAACGA
CCAGAGCGATCGTCTTCTGATCAAAGGAGGTAAAATTGTTAATGATGACCAGTCGTTCTATGCAGACATA
TACATGGAAGATGGGTTGATCAAGCAAATAGGAGAAAATCTGATTGTGCCAGGAGGAGTGAAGACCATCG
AGGCCCACTCCCGGATGGTGATCCCCGGAGGAATTGACGTCCACACTCGTTTCCAGATGCCTGATCAGGG
AATGACGTCTGCTGATGATTTCTTCCAAGGAACCAAGGCGGCCCTGGCTGGGGGAACCACTATGATCATT
GACCACGTTGTTCCTGAGCCTGGGACAAGCCTGCTCGCTGCCTTTGACCAGTGGAGGGAATGGGCCGACA
GCAAGTCCTGCTGTGACTACTCTCTGCATGTGGACATCAGTGAGTGGCATAAGGGCATCCAGGAGGAGAT
GGAAGCGCTTGTGAAGGATCACGGGGTAAATTCCTTCCTCGTGTACATGGCTTTCAAAGATCGCTTCCAG
CTAACGGATTGCCAGATTTATGAAGTACTGAGTGTGATCCGGGATATTGGCGCCATAGCCCAAGTCCACG
CAGAAAATGGCGACATCATTGCAGAGGAGCAGCAGAGGATCCTGGATCTGGGCATCACGGGCCCCGAGGG
ACATGTGCTGAGCCGACCTGAGGAGGTCGAGGCCGAAGCCGTGAATCGTGCCATCACCATCGCCAACCAG
ACCAACTGCCCGCTGTATATCACCAAGGTGATGAGCAAAAGCTCTGCTGAGGTCATCGCCCAGGCACGGA
AGAAGGGAACTGTGGTGTATGGCGAGCCCATCACTGCCAGCTTGGGAACGGACGGCTCCCATTACTGGAG
CAAGAACTGGGCCAAGGCTGCTGCCTTTGTCACCTCCCCACCCTTGAGCCCTGATCCAACCACTCCAGAC
TTTCTCAACTCCTTGCTGTCCTGTGGAGACCTCCAGGTCACGGGCAGTGCCCATTGCACGTTTAACACTG
CCCAGAAGGCTGTAGGAAAGGACAACTTCACCCTGATTCCGGAGGGCACCAATGGCACTGAGGAGCGGAT
GTCCGTCATCTGGGACAAGGCTGTGGTCACTGGGAAGATGGATGAGAACCAGTTTGTGGCTGTGACCAGC
ACCAATGCAGCCAAAGTCTTCAACCTTTACCCCCGGAAAGGCCGCATTGCTGTGGGATCCGATGCCGACC
TGGTCATCTGGGACCCCGACAGCGTTAAAACCATCTCTGCCAAGACACACAACAGCTCTCTCGAGTACAA
CATCTTTGAAGGCATGGAGTGCCGCGGCTCCCCACTGGTGGTCATCAGCCAGGGGAAGATTGTCCTGGAG
GACGGCACCCTGCATGTCACCGAAGGCTCTGGACGCTACATTCCCCGGAAGCCCTTCCCTGATTTTGTTT
ACAAGCGTATCAAGGCAAGGAGCAGGCTGGCTGAGCTGAGAGGGGTTCCTCGTGGCCTGTATGACGGACC
TGTGTGTGAAGTGTCTGTGACGCCCAAGACAGTCACTCCAGCCTCCTCGGCCAAGACGTCTCCTGCCAAG
CAGCAGGCCCCACCTGTCCGGAACCTGCACCAGTCTGGATTCAGTTTGTCTGGTGCTCAGATTGATGACA
ACATTCCCCGCCGCACCACCCAGCGTATCGTGGCGCCCCCCGGTGGCCGTGCCAACATCACCAGCCTGGG
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC231368 representing NM_001197293
Red=Cloning site Green=Tags(s)

MAERKQSGKAAEDEEVPAFFKNLGSGSPKPRQKFCGMFCPVEGSSENKTIDFDSLSVGRGSGQVVAQQRD
VAHLGPDPQPPYSRQGRRAGGEPSVESGRKVEIRRASGKEALQNINDQSDRLLIKGGKIVNDDQSFYADI
YMEDGLIKQIGENLIVPGGVKTIEAHSRMVIPGGIDVHTRFQMPDQGMTSADDFFQGTKAALAGGTTMII
DHVVPEPGTSLLAAFDQWREWADSKSCCDYSLHVDISEWHKGIQEEMEALVKDHGVNSFLVYMAFKDRFQ
LTDCQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQRILDLGITGPEGHVLSRPEEVEAEAVNRAITIANQ
TNCPLYITKVMSKSSAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPD
FLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTS
TNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLE
DGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAK
QQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001197293
ORF Size 2031 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001197293.3
RefSeq ORF 2034 bp
Locus ID 1808
UniProt ID Q16555
Cytogenetics 8p21.2
Protein Families Druggable Genome
Protein Pathways Axon guidance
MW 74 kDa
Gene Summary This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Other Versions

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