CRMP2 (DPYSL2) (NM_001197293) Human Tagged ORF Clone

CAT#: RC231368

DPYSL2 (Myc-DDK-tagged)-Human dihydropyrimidinase-like 2 (DPYSL2), transcript variant 1

Product Images

Circular map for RC231368

• 

  Western blot validation of overexpression lysate (Cat# LY434367) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231368 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRMP2
• Synonyms: CRMP-2; CRMP2; DHPRP2; DRP-2; DRP2; N2A3; ULIP-2; ULIP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231368 representing NM_001197293
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGAGAAAGCAATCCGGGAAGGCGGCAGAGGACGAAGAGGTCCCTGCTTTTTTTAAAAACCTGG
  GCTCCGGCAGCCCCAAGCCCCGGCAGAAATTCTGTGGCATGTTCTGCCCGGTGGAAGGGTCCTCGGAGAA
  CAAGACCATCGACTTCGACTCGCTGTCGGTGGGCCGGGGCTCGGGGCAGGTGGTGGCTCAGCAGCGGGAC
  GTCGCCCACTTGGGCCCGGACCCGCAGCCGCCGTACTCGCGGCAGGGCCGGCGCGCCGGCGGAGAGCCAT
  CTGTTGAATCGGGCCGGAAGGTGGAGATCCGGAGGGCCTCGGGCAAAGAAGCCCTGCAGAACATCAACGA
  CCAGAGCGATCGTCTTCTGATCAAAGGAGGTAAAATTGTTAATGATGACCAGTCGTTCTATGCAGACATA
  TACATGGAAGATGGGTTGATCAAGCAAATAGGAGAAAATCTGATTGTGCCAGGAGGAGTGAAGACCATCG
  AGGCCCACTCCCGGATGGTGATCCCCGGAGGAATTGACGTCCACACTCGTTTCCAGATGCCTGATCAGGG
  AATGACGTCTGCTGATGATTTCTTCCAAGGAACCAAGGCGGCCCTGGCTGGGGGAACCACTATGATCATT
  GACCACGTTGTTCCTGAGCCTGGGACAAGCCTGCTCGCTGCCTTTGACCAGTGGAGGGAATGGGCCGACA
  GCAAGTCCTGCTGTGACTACTCTCTGCATGTGGACATCAGTGAGTGGCATAAGGGCATCCAGGAGGAGAT
  GGAAGCGCTTGTGAAGGATCACGGGGTAAATTCCTTCCTCGTGTACATGGCTTTCAAAGATCGCTTCCAG
  CTAACGGATTGCCAGATTTATGAAGTACTGAGTGTGATCCGGGATATTGGCGCCATAGCCCAAGTCCACG
  CAGAAAATGGCGACATCATTGCAGAGGAGCAGCAGAGGATCCTGGATCTGGGCATCACGGGCCCCGAGGG
  ACATGTGCTGAGCCGACCTGAGGAGGTCGAGGCCGAAGCCGTGAATCGTGCCATCACCATCGCCAACCAG
  ACCAACTGCCCGCTGTATATCACCAAGGTGATGAGCAAAAGCTCTGCTGAGGTCATCGCCCAGGCACGGA
  AGAAGGGAACTGTGGTGTATGGCGAGCCCATCACTGCCAGCTTGGGAACGGACGGCTCCCATTACTGGAG
  CAAGAACTGGGCCAAGGCTGCTGCCTTTGTCACCTCCCCACCCTTGAGCCCTGATCCAACCACTCCAGAC
  TTTCTCAACTCCTTGCTGTCCTGTGGAGACCTCCAGGTCACGGGCAGTGCCCATTGCACGTTTAACACTG
  CCCAGAAGGCTGTAGGAAAGGACAACTTCACCCTGATTCCGGAGGGCACCAATGGCACTGAGGAGCGGAT
  GTCCGTCATCTGGGACAAGGCTGTGGTCACTGGGAAGATGGATGAGAACCAGTTTGTGGCTGTGACCAGC
  ACCAATGCAGCCAAAGTCTTCAACCTTTACCCCCGGAAAGGCCGCATTGCTGTGGGATCCGATGCCGACC
  TGGTCATCTGGGACCCCGACAGCGTTAAAACCATCTCTGCCAAGACACACAACAGCTCTCTCGAGTACAA
  CATCTTTGAAGGCATGGAGTGCCGCGGCTCCCCACTGGTGGTCATCAGCCAGGGGAAGATTGTCCTGGAG
  GACGGCACCCTGCATGTCACCGAAGGCTCTGGACGCTACATTCCCCGGAAGCCCTTCCCTGATTTTGTTT
  ACAAGCGTATCAAGGCAAGGAGCAGGCTGGCTGAGCTGAGAGGGGTTCCTCGTGGCCTGTATGACGGACC
  TGTGTGTGAAGTGTCTGTGACGCCCAAGACAGTCACTCCAGCCTCCTCGGCCAAGACGTCTCCTGCCAAG
  CAGCAGGCCCCACCTGTCCGGAACCTGCACCAGTCTGGATTCAGTTTGTCTGGTGCTCAGATTGATGACA
  ACATTCCCCGCCGCACCACCCAGCGTATCGTGGCGCCCCCCGGTGGCCGTGCCAACATCACCAGCCTGGG
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231368 representing NM_001197293
  Red=Cloning site Green=Tags(s)
  
  MAERKQSGKAAEDEEVPAFFKNLGSGSPKPRQKFCGMFCPVEGSSENKTIDFDSLSVGRGSGQVVAQQRD
  VAHLGPDPQPPYSRQGRRAGGEPSVESGRKVEIRRASGKEALQNINDQSDRLLIKGGKIVNDDQSFYADI
  YMEDGLIKQIGENLIVPGGVKTIEAHSRMVIPGGIDVHTRFQMPDQGMTSADDFFQGTKAALAGGTTMII
  DHVVPEPGTSLLAAFDQWREWADSKSCCDYSLHVDISEWHKGIQEEMEALVKDHGVNSFLVYMAFKDRFQ
  LTDCQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQRILDLGITGPEGHVLSRPEEVEAEAVNRAITIANQ
  TNCPLYITKVMSKSSAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPD
  FLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTS
  TNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLE
  DGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAK
  QQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001197293
• ORF Size: 2031 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001197293.3
• RefSeq ORF: 2034 bp
• Locus ID: 1808
• UniProt ID: Q16555
• Cytogenetics: 8p21.2
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• MW: 74 kDa
• Gene Summary: This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]