Golgin subfamily A member 4 (GOLGA4) (NM_001172713) Human Tagged ORF Clone

CAT#: RC230747

GOLGA4 (Myc-DDK-tagged)-Human golgin A4 (GOLGA4), transcript variant 1

Product Images

Circular map for RC230747

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Golgin subfamily A member 4
• Synonyms: CRPF46; GCP2; GOLG; MU-RMS-40.18; p230
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230747 representing NM_001172713
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAAGAAACTGAAGCAAAAGATCAGCGAGGAGCAGCAGCAGCTCCAGCAGGCGCTGGCTCCTGCTC
  AGGCGTCCTCCAATTCTTCAACACCAACAAGAATGAGGAGCAGGACATCTTCATTTACAGAGCAACTTGA
  TGAAGGTACACCCAATAGAGAGAATGCATCTACTCATGCCTCGAAATCTCCTGACAGTGTTAATGGAAGT
  GAACCAAGCATTCCTCAGTCAGGTGACACACAGTCTTTTGCACAGAAGCTCCAGCTCCGGGTGCCCTCCG
  TGGAGTCTTTGTTTCGAAGTCCGATAAAGGAATCTCTATTCCGGTCTTCTTCTAAAGAGTCTTTGGTACG
  AACATCTTCCAGAGAATCCCTGAATCGACTTGACCTGGACAGTTCTACTGCCAGTTTTGATCCACCCTCT
  GATATGGATAGCGAGGCTGAAGACTTGGTAGGGAATTCAGACAGTCTCAACAAAGAACAGTTGATTCAGC
  GGTTGCGAAGAATGGAACGAAGCTTAAGTAGCTACAGGGGAAAATATTCTGAGCTTGTTACAGCTTATCA
  GATGCTTCAGAGAGAGAAGAAAAAGCTACAAGGTATATTAAGTCAGAGTCAGGATAAATCACTTCGGAGA
  ATAGCAGAATTAAGAGAGGAGCTCCAAATGGACCAGCAGGCAAAGAAACATCTGCAAGAGGAGTTTGATG
  CATCTTTAGAGGAGAAAGATCAGTATATCAGTGTTCTCCAAACTCAGGTTTCTCTACTGAAACAACGATT
  ACGAAATGGCCCGATGAATGTTGATGTACTGAAACCACTTCCTCAGCTGGAACCACAGGCTGAAGTCTTC
  ACTAAAGAAGAGAATCCAGAAAGTGATGGAGAGCCAGTAGTGGAAGATGGAACTTCTGTAAAAACACTGG
  AAACACTCCAGCAAAGAGTGAAGCGTCAAGAGAACCTACTTAAGCGTTGTAAGGAAACAATTCAGTCACA
  TAAGGAACAATGTACACTATTAACTAGTGAAAAAGAAGCTCTGCAAGAACAACTGGATGAAAGACTTCAA
  GAACTAGAAAAGATAAAGGACCTTCATATGGCCGAGAAGACTAAACTTATCACTCAGTTGCGTGATGCAA
  AGAACTTAATTGAACAGCTTGAACAAGATAAGGGAATGGTAATCGCAGAGACAAAACGTCAGATGCATGA
  AACCCTGGAAATGAAAGAAGAAGAAATTGCTCAACTCCGTAGTCGCATCAAACAGATGACTACCCAGGGA
  GAGGAATTACGGGAACAGAAAGAAAAGTCCGAAAGAGCTGCTTTTGAGGAACTTGAAAAAGCTTTGAGTA
  CAGCCCAAAAAACAGAGGAAGCACGGAGAAAACTGAAGGCAGAAATGGATGAACAAATAAAAACTATCGA
  AAAAACAAGTGAGGAGGAACGCATCAGTCTTCAACAGGAATTAAGTCGGGTGAAACAGGAGGTTGTTGAT
  GTAATGAAAAAATCCTCAGAAGAACAAATTGCTAAGCTACAGAAGCTTCATGAAAAGGAGCTGGCCAGAA
  AAGAGCAGGAACTGACCAAGAAGCTTCAGACCCGAGAAAGGGAATTTCAGGAACAAATGAAAGTAGCTCT
  TGAAAAGAGTCAATCAGAATATTTGAAGATCAGCCAAGAAAAAGAACAGCAAGAATCTTTGGCCCTAGAA
  GAGTTAGAGTTGCAGAAAAAAGCAATCCTCACAGAAAGTGAAAATAAACTTCGGGACCTTCAGCAAGAAG
  CAGAGACTTACAGAACTAGAATTCTTGAATTGGAAAGTTCTTTGGAAAAAAGCTTACAAGAAAACAAAAA
  TCAGTCAAAAGATTTGGCTGTTCATCTGGAAGCTGAAAAAAATAAGCACAATAAGGAGATTACAGTCATG
  GTTGAAAAACACAAGACAGAATTGGAAAGCCTTAAGCATCAGCAGGATGCCCTTTGGACTGAAAAACTCC
  AAGTCTTAAAGCAACAATATCAGACTGAAATGGAAAAACTTAGGGAAAAGTGTGAACAAGAAAAAGAAAC
  ATTGTTGAAAGACAAAGAGATTATCTTCCAGGCCCACATAGAAGAAATGAATGAAAAGACTTTAGAAAAG
  CTTGATGTGAAGCAAACAGAACTAGAATCATTATCTTCTGAACTGTCAGAAGTATTAAAAGCCCGTCACA
  AACTAGAAGAGGAACTTTCTGTTCTGAAAGATCAAACAGATAAAATGAAGCAGGAATTAGAGGCCAAGAT
  GGATGAACAGAAAAATCATCACCAGCAGCAAGTTGACAGTATCATTAAAGAACACGAGGTATCTATCCAG
  AGGACTGAGAAGGCATTAAAAGATCAAATTAATCAACTTGAGCTTCTCTTGAAGGAAAGGGACAAGCATT
  TGAAAGAGCATCAGGCTCATGTAGAAAATTTAGAGGCAGATATTAAAAGGTCTGAAGGGGAACTCCAGCA
  GGCATCTGCTAAGCTGGACGTTTTTCAGTCTTACCAGAGTGCCACACATGAGCAGACAAAAGCATATGAG
  GAACAGTTGGCCCAATTGCAGCAGAAGTTGTTGGATTTGGAAACAGAAAGAATTCTTCTTACCAAACAGG
  TTGCTGAAGTTGAAGCACAAAAGAAAGATGTTTGTACTGAGTTAGATGCTCACAAAATCCAGGTGCAGGA
  CTTAATGCAGCAACTTGAAAAACAAAATAGTGAAATGGAGCAAAAAGTAAAATCTTTAACCCAAGTCTAT
  GAGTCCAAACTTGAAGATGGTAACAAAGAACAGGAACAGACAAAGCAAATCTTGGTGGAAAAGGAAAATA
  TGATTTTACAAATGAGAGAAGGACAGAAGAAAGAAATTGAGATACTCACACAGAAATTGTCAGCCAAGGA
  GGACAGTATTCATATTTTGAATGAGGAATATGAAACCAAATTTAAAAACCAAGAAAAAAAGATGGAAAAA
  GTTAAGCAGAAAGCAAAGGAGATGCAAGAAACGTTAAAGAAAAAATTACTGGATCAGGAAGCCAAACTTA
  AGAAAGAGCTTGAAAATACTGCTCTAGAGCTTAGTCAGAAAGAAAAACAGTTTAATGCCAAAATGCTGGA
  AATGGCACAGGCTAACTCAGCTGGAATCAGTGATGCAGTGTCAAGACTGGAAACAAACCAAAAAGAACAA
  ATAGAAAGTCTTACTGAGGTTCATCGACGAGAACTCAATGATGTCATATCAATCTGGGAAAAGAAACTTA
  ATCAGCAAGCTGAAGAACTTCAGGAAATACATGAAATCCAATTACAGGAAAAAGAACAAGAGGTAGCAGA
  ACTGAAACAAAAGATCCTCCTATTTGGGTGTGAAAAAGAAGAGATGAACAAGGAAATAACATGGCTGAAG
  GAAGAAGGTGTTAAGCAGGATACAACATTAAATGAATTACAGGAACAGTTAAAGCAGAAGTCTGCCCATG
  TGAATTCTCTTGCACAAGATGAAACTAAACTGAAAGCTCATCTTGAAAAGCTAGAGGTTGACTTGAATAA
  GTCTCTGAAGGAAAATACTTTTCTTCAAGAGCAGCTAGTTGAACTGAAGATGCTGGCAGAAGAAGATAAG
  CGGAAGGTTTCTGAGTTGACTAGCAAGTTGAAAACCACAGATGAAGAATTCCAGAGTTTGAAATCTTCAC
  ATGAAAAAAGTAACAAAAGCCTAGAGGACAAGAGCTTGGAATTTAAAAAACTGTCTGAGGAACTAGCGAT
  TCAGCTAGATATTTGCTGTAAGAAAACCGAAGCCTTATTAGAAGCTAAAACAAATGAGCTAATCAACATT
  AGTAGTAGTAAAACTAATGCCATTCTTTCTAGGATTTCTCATTGTCAGCACCGTACAACTAAAGTTAAGG
  AGGCACTGTTAATTAAAACTTGCACAGTTTCTGAATTAGAAGCACAACTTAGACAGTTGACAGAGGAGCA
  AAATACACTAAATATTTCTTTTCAACAGGCTACTCATCAGTTAGAAGAAAAAGAAAATCAAATTAAGAGC
  ATGAAGGCTGATATTGAAAGTCTTGTAACAGAAAAAGAAGCCTTACAGAAGGAAGGAGGCAATCAGCAAC
  AGGCTGCTTCTGAAAAGGAGTCTTGTATAACACAGTTGAAGAAAGAGTTATCTGAAAACATCAATGCTGT
  CACATTGATGAAAGAAGAGCTTAAAGAAAAAAAAGTTGAGATTAGCAGTCTTAGTAAACAACTAACTGAT
  TTGAATGTTCAGCTTCAAAATAGCATCAGCCTATCCGAAAAAGAAGCAGCCATTTCATCACTAAGAAAGC
  AGTATGATGAAGAAAAATGTGAATTGCTGGATCAGGTGCAAGATTTATCTTTTAAAGTTGACACTCTGAG
  TAAAGAGAAAATTTCTGCTCTTGAGCAGGTAGATGACTGGTCCAATAAATTCTCAGAATGGAAGAAGAAA
  GCACAGTCAAGATTTACACAGCATCAAAACACTGTTAAAGAATTGCAGATCCAGCTTGAGTTAAAATCAA
  AGGAAGCTTATGAAAAGGATGAGCAGATAAATTTATTGAAGGAAGAGCTTGATCAGCAAAATAAAAGATT
  TGATTGTTTAAAGGGTGAAATGGAAGACGACAAGAGCAAGATGGAGAAAAAGGAGTCTAATTTAGAAACA
  GAGTTAAAGTCTCAAACAGCAAGAATTATGGAATTAGAGGACCATATTACCCAGAAAACTATTGAAATAG
  AGTCCTTAAATGAAGTTCTTAAAAATTACAATCAACAAAAGGATATTGAACACAAAGAATTGGTTCAGAA
  ACTTCAACATTTTCAAGAGTTAGGAGAAGAAAAGGACAACAGGGTTAAAGAAGCTGAAGAAAAAATCTTA
  ACACTTGAAAACCAAGTTTATTCCATGAAAGCTGAACTTGAAACTAAGAAGAAAGAATTAGAACATGTGA
  ATTTAAGTGTGAAAAGCAAAGAGGAGGAGTTAAAGGCATTGGAAGATAGGCTTGAGTCAGAAAGTGCTGC
  AAAATTAGCAGAGTTGAAGAGAAAAGCTGAACAAAAAATTGCTGCCATTAAGAAGCAGTTGTTATCTCAA
  ATGGAAGAGAAAGAAGAACAGTATAAAAAAGGTACAGAAAGCCATTTGAGTGAGCTAAATACAAAATTGC
  AGGAAAGAGAAAGGGAAGTTCACATCTTGGAAGAAAAACTTAAGTCAGTGGAAAGTTCACAGTCAGAAAC
  ATTAATTGTACCCAGATCAGCAAAAAATGTGGCAGCATATACTGAACAAGAAGAAGCAGATTCCCAAGGC
  TGTGTGCAGAAGACATATGAAGAAAAAATCAGTGTTTTACAAAGAAACTTAACTGAAAAAGAAAAGCTAT
  TGCAGAGGGTAGGGCAGGAAAAAGAAGAGACAGTTTCTTCTCATTTTGAAATGCGATGCCAATACCAGGA
  GCGCTTAATAAAGCTAGAACATGCTGAGGCAAAGCAACATGAAGATCAAAGTATGATAGGTCATCTTCAA
  GAGGAGCTTGAAGAAAAAAACAAGAAATATTCCTTGATAGTAGCCCAGCATGTGGAAAAAGAAGGAGGTA
  AAAATAACATACAGGCAAAGCAAAACTTGGAAAATGTGTTTGACGACGTCCAGAAAACCCTCCAGGAGAA
  GGAACTAACCTGTCAGATTTTGGAGCAAAAGATAAAAGAGCTGGATTCCTGCTTAGTAAGACAGAAAGAA
  GTACATAGAGTTGAAATGGAAGAGTTGACCTCAAAATATGAAAAATTACAGGCTTTACAACAGATGGATG
  GAAGAAATAAACCCACAGAACTTTTGGAAGAAAACACTGAAGAAAAGTCCAAATCACATTTGGTCCAACC
  CAAATTGCTTAGTAACATGGAAGCCCAGCACAATGATCTGGAGTTTAAATTAGCCGGGGCAGAACGGGAG
  AAACAGAAACTGGGCAAGGAGATTGTTAGATTGCAGAAAGACCTTCGAATGTTGAGAAAGGAGCATCAGC
  AAGAATTGGAAATACTAAAGAAAGAATATGATCAAGAAAGGGAAGAGAAAATCAAACAGGAGCAGGAAGA
  TCTTGAACTGAAGCACAATTCCACATTAAAACAGCTGATGAGGGAGTTTAATACACAGCTGGCACAAAAG
  GAACAAGAGCTGGAAATGACCATAAAAGAAACTATCAATAAGGCCCAGGAGGTGGAGGCTGAACTTTTAG
  AAAGCCATCAAGAAGAGACAAATCAGTTACTTAAAAAAATTGCTGAGAAAGATGATGATCTAAAACGAAC
  AGCCAAAAGATATGAAGAAATCCTTGATGCTCGTGAAGAAGAAATGACTGCAAAAGTAAGGGACCTGCAG
  ACTCAACTTGAGGAGCTGCAGAAGAAATACCAGCAAAAGCTAGAGCAGGAGGAGAACCCTGGCAATGATA
  ATACACAGCTAGCACAGAAGACGACTTTAATCAGTGATTCGAAATTGAAAGAGCAAGAGTTCAGAGAACA
  GATTCACAATTTAGAAGACCGTTTGAAGAAATATGAAAAGAATGTATATGCAACAACTGTGGGGACACCT
  TACAAAGGTGGCAATTTGTACCATACGGATGTCTCACTCTTTGGAGAACCTACCGAATTTGAGTATTTGC
  GAAAAGTGCTTTTTGAGTATATGATGGGTCGTGAGACTAAGACCATGGCAAAAGTTATAACCACCGTACT
  GAAGTTCCCTGATGATCAGACTCAGAAAATTTTGGAAAGAGAAGATGCTCGGCTGATGTCATGGCTCCGA
  TCTTCATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230747 representing NM_001172713
  Red=Cloning site Green=Tags(s)
  
  MFKKLKQKISEEQQQLQQALAPAQASSNSSTPTRMRSRTSSFTEQLDEGTPNRENASTHASKSPDSVNGS
  EPSIPQSGDTQSFAQKLQLRVPSVESLFRSPIKESLFRSSSKESLVRTSSRESLNRLDLDSSTASFDPPS
  DMDSEAEDLVGNSDSLNKEQLIQRLRRMERSLSSYRGKYSELVTAYQMLQREKKKLQGILSQSQDKSLRR
  IAELREELQMDQQAKKHLQEEFDASLEEKDQYISVLQTQVSLLKQRLRNGPMNVDVLKPLPQLEPQAEVF
  TKEENPESDGEPVVEDGTSVKTLETLQQRVKRQENLLKRCKETIQSHKEQCTLLTSEKEALQEQLDERLQ
  ELEKIKDLHMAEKTKLITQLRDAKNLIEQLEQDKGMVIAETKRQMHETLEMKEEEIAQLRSRIKQMTTQG
  EELREQKEKSERAAFEELEKALSTAQKTEEARRKLKAEMDEQIKTIEKTSEEERISLQQELSRVKQEVVD
  VMKKSSEEQIAKLQKLHEKELARKEQELTKKLQTREREFQEQMKVALEKSQSEYLKISQEKEQQESLALE
  ELELQKKAILTESENKLRDLQQEAETYRTRILELESSLEKSLQENKNQSKDLAVHLEAEKNKHNKEITVM
  VEKHKTELESLKHQQDALWTEKLQVLKQQYQTEMEKLREKCEQEKETLLKDKEIIFQAHIEEMNEKTLEK
  LDVKQTELESLSSELSEVLKARHKLEEELSVLKDQTDKMKQELEAKMDEQKNHHQQQVDSIIKEHEVSIQ
  RTEKALKDQINQLELLLKERDKHLKEHQAHVENLEADIKRSEGELQQASAKLDVFQSYQSATHEQTKAYE
  EQLAQLQQKLLDLETERILLTKQVAEVEAQKKDVCTELDAHKIQVQDLMQQLEKQNSEMEQKVKSLTQVY
  ESKLEDGNKEQEQTKQILVEKENMILQMREGQKKEIEILTQKLSAKEDSIHILNEEYETKFKNQEKKMEK
  VKQKAKEMQETLKKKLLDQEAKLKKELENTALELSQKEKQFNAKMLEMAQANSAGISDAVSRLETNQKEQ
  IESLTEVHRRELNDVISIWEKKLNQQAEELQEIHEIQLQEKEQEVAELKQKILLFGCEKEEMNKEITWLK
  EEGVKQDTTLNELQEQLKQKSAHVNSLAQDETKLKAHLEKLEVDLNKSLKENTFLQEQLVELKMLAEEDK
  RKVSELTSKLKTTDEEFQSLKSSHEKSNKSLEDKSLEFKKLSEELAIQLDICCKKTEALLEAKTNELINI
  SSSKTNAILSRISHCQHRTTKVKEALLIKTCTVSELEAQLRQLTEEQNTLNISFQQATHQLEEKENQIKS
  MKADIESLVTEKEALQKEGGNQQQAASEKESCITQLKKELSENINAVTLMKEELKEKKVEISSLSKQLTD
  LNVQLQNSISLSEKEAAISSLRKQYDEEKCELLDQVQDLSFKVDTLSKEKISALEQVDDWSNKFSEWKKK
  AQSRFTQHQNTVKELQIQLELKSKEAYEKDEQINLLKEELDQQNKRFDCLKGEMEDDKSKMEKKESNLET
  ELKSQTARIMELEDHITQKTIEIESLNEVLKNYNQQKDIEHKELVQKLQHFQELGEEKDNRVKEAEEKIL
  TLENQVYSMKAELETKKKELEHVNLSVKSKEEELKALEDRLESESAAKLAELKRKAEQKIAAIKKQLLSQ
  MEEKEEQYKKGTESHLSELNTKLQEREREVHILEEKLKSVESSQSETLIVPRSAKNVAAYTEQEEADSQG
  CVQKTYEEKISVLQRNLTEKEKLLQRVGQEKEETVSSHFEMRCQYQERLIKLEHAEAKQHEDQSMIGHLQ
  EELEEKNKKYSLIVAQHVEKEGGKNNIQAKQNLENVFDDVQKTLQEKELTCQILEQKIKELDSCLVRQKE
  VHRVEMEELTSKYEKLQALQQMDGRNKPTELLEENTEEKSKSHLVQPKLLSNMEAQHNDLEFKLAGAERE
  KQKLGKEIVRLQKDLRMLRKEHQQELEILKKEYDQEREEKIKQEQEDLELKHNSTLKQLMREFNTQLAQK
  EQELEMTIKETINKAQEVEAELLESHQEETNQLLKKIAEKDDDLKRTAKRYEEILDAREEEMTAKVRDLQ
  TQLEELQKKYQQKLEQEENPGNDNTQLAQKTTLISDSKLKEQEFREQIHNLEDRLKKYEKNVYATTVGTP
  YKGGNLYHTDVSLFGEPTEFEYLRKVLFEYMMGRETKTMAKVITTVLKFPDDQTQKILEREDARLMSWLR
  SSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172713
• ORF Size: 6729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172713.2
• RefSeq ORF: 6732 bp
• Locus ID: 2803
• UniProt ID: Q13439
• Cytogenetics: 3p22.2
• MW: 262.8 kDa
• Gene Summary: The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein has been postulated to play a role in Rab6-regulated membrane-tethering events in the Golgi apparatus. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010]