ZNF540 (NM_001172226) Human Tagged ORF Clone

CAT#: RC230367

ZNF540 (Myc-DDK-tagged)-Human zinc finger protein 540 (ZNF540), transcript variant 3. Note: ORF is codon optimized

Product Images

Circular map for RC230367

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF540
• Synonyms: Nbla10512
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230367 ORF sequence, codon optimized.
  Due to the complexity of NM_001172226, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACATGCTCTTGTGACATTCAGAGACGTTGCGATTGATTTTTCACAGAAAGAATGGGAATGCCTCG
  ATACAACTCAGCGGAAGCTTTATCGGGACGTGATGCTGGAGAACTATAATAACCTTGTGAGCCTCGGACT
  GCTCAGCCGGCATAAGACTAAGAAATTGAGCAGTGAAAAAGACATCCACGAGATCTCACTCTCAAAGGAA
  TCAATCATAGAAAAGTCAAAGACCCTGCGACTGAAGGGGTCCATATTCAGAAACGAGTGGCAAAATAAAA
  GTGAGTTTGAGGGTCAGCAGGGTCTTAAGGAGAGGAGCATCTCTCAGAAAAAAATCGTTTCTAAGAAAAT
  GTCAACAGACCGAAAGCGGCCTTCCTTCACGCTCAATCAGCGGATTCACAACTCTGAGAAGTCATGTGAT
  AGCCACTTGGTACAGCATGGCAAGATTGATAGTGATGTGAAGCACGATTGTAAGGAGTGCGGCAGCACGT
  TCAACAATGTGTACCAGCTTACATTGCATCAGAAGATACATACCGGAGAAAAGTCTTGCAAATGTGAAAA
  ATGCGGCAAAGTGTTCTCCCACAGCTACCAGCTGACCCTGCATCAGAGGTTCCACACCGGGGAAAAGCCC
  TATGAGTGCCAGGAATGTGGTAAGACATTTACTTTGTATCCACAGTTGAACCGCCATCAAAAAATCCACA
  CCGGTAAGAAACCCTATATGTGCAAAAAGTGCGACAAAGGGTTTTTTTCTCGCCTGGAGCTGACACAGCA
  CAAAAGGATCCACACCGGGAAAAAGAGCTACGAATGTAAGGAGTGCGGTAAGGTGTTCCAACTTATATTC
  TACTTTAAAGAACACGAGCGAATCCACACTGGCAAAAAGCCATACGAATGTAAGGAGTGCGGCAAAGCCT
  TCTCAGTTTGCGGCCAGCTGACCAGGCACCAGAAAATTCACACAGGCGTGAAGCCCTACGAATGCAAAGA
  GTGCGGAAAAACCTTCAGACTTAGTTTCTACCTGACTGAACATCGGCGCACTCACGCCGGAAAAAAGCCC
  TACGAGTGCAAGGAATGCGGAAAATCCTTTAACGTGCGGGGCCAGCTTAATAGACATAAGACCATCCACA
  CTGGAATAAAACCGTTCGCTTGTAAAGTTTGCGAGAAAGCGTTCTCTTATTCTGGAGACTTGCGAGTCCA
  TTCTAGAATCCATACAGGCGAGAAACCCTATGAATGTAAGGAGTGTGGCAAGGCGTTCATGCTGCGAAGC
  GTCCTGACGGAGCACCAGCGCTTGCATACTGGGGTGAAGCCCTATGAGTGTAAAGAATGCGGCAAAACAT
  TCCGGGTGCGCAGCCAGATCAGCCTTCACAAGAAGATTCATACAGACGTGAAGCCATATAAATGTGTTAG
  GTGTGGTAAAACTTTTAGGTTCGGGTTCTATCTGACCGAACATCAGAGAATTCACACTGGGGAAAAACCC
  TACAAGTGCAAAGAATGCGGGAAGGCATTCATCCGGCGGGGAAATCTTAAAGAACATCTGAAGATCCACT
  CCGGTTTGAAGCCATATGACTGTAAGGAATGCGGGAAAAGTTTTTCACGACGGGGGCAGTTCACAGAGCA
  CCAGAAAATCCACACTGGAGTCAAGCCCTATAAATGTAAAGAGTGCGGAAAGGCTTTTAGCCGATCAGTT
  GATCTGAGAATCCATCAGCGGATCCATACCGGTGAGAAACCCTATGAATGCAAACAGTGCGGCAAGGCCT
  TCCGCTTGAATAGCCATCTGACAGAACATCAGCGGATACATACAGGAGAAAAGCCTTATGAATGCAAGGT
  TTGTAGAAAGGCTTTTCGCCAGTACAGCCACCTGTATCAACATCAGAAGACACATAACGTCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230367 representing NM_001172226
  Red=Cloning site Green=Tags(s)
  
  MAHALVTFRDVAIDFSQKEWECLDTTQRKLYRDVMLENYNNLVSLGLLSRHKTKKLSSEKDIHEISLSKE
  SIIEKSKTLRLKGSIFRNEWQNKSEFEGQQGLKERSISQKKIVSKKMSTDRKRPSFTLNQRIHNSEKSCD
  SHLVQHGKIDSDVKHDCKECGSTFNNVYQLTLHQKIHTGEKSCKCEKCGKVFSHSYQLTLHQRFHTGEKP
  YECQECGKTFTLYPQLNRHQKIHTGKKPYMCKKCDKGFFSRLELTQHKRIHTGKKSYECKECGKVFQLIF
  YFKEHERIHTGKKPYECKECGKAFSVCGQLTRHQKIHTGVKPYECKECGKTFRLSFYLTEHRRTHAGKKP
  YECKECGKSFNVRGQLNRHKTIHTGIKPFACKVCEKAFSYSGDLRVHSRIHTGEKPYECKECGKAFMLRS
  VLTEHQRLHTGVKPYECKECGKTFRVRSQISLHKKIHTDVKPYKCVRCGKTFRFGFYLTEHQRIHTGEKP
  YKCKECGKAFIRRGNLKEHLKIHSGLKPYDCKECGKSFSRRGQFTEHQKIHTGVKPYKCKECGKAFSRSV
  DLRIHQRIHTGEKPYECKQCGKAFRLNSHLTEHQRIHTGEKPYECKVCRKAFRQYSHLYQHQKTHNVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172226
• ORF Size: 1884 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172226.1, NM_001172226.2, NP_001165697.1
• RefSeq Size: 3141 bp
• RefSeq ORF: 1887 bp
• Locus ID: 163255
• UniProt ID: Q8NDQ6
• Cytogenetics: 19q13.12
• Protein Families: Transcription Factors
• MW: 73.7 kDa
• Gene Summary: May act as a transcriptional repressor.[UniProtKB/Swiss-Prot Function]