SP110 (NM_001185015) Human Tagged ORF Clone

CAT#: RC230264

SP110 (Myc-DDK-tagged)-Human SP110 nuclear body protein (SP110), transcript variant d

Product Images

Circular map for RC230264

• 

  Western blot validation of overexpression lysate (Cat# LY433264) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230264 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SP110
• Synonyms: IFI41; IFI75; IPR1; VODI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230264 representing NM_001185015
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGGGGCTTCAGGATGTTCACCATGACAAGAGCCATGGAAGAGGCTCTTTTTCAGCACTTCATGC
  ACCAGAAGCTGGGGATCGCCTATGCCATACACAAGCCATTTCCCTTCTTTGAAGGCCTCCTAGACAACTC
  CATCATCACTAAGAGAATGTACATGGAATCTCTGGAAGCCTGTAGAAATTTGATCCCTGTATCCAGAGTG
  GTGCACAACATTCTCACCCAACTGGAGAGGACTTTTAACCTGTCTCTTCTGGTGACATTGTTCAGTCAAA
  TTAACCTGCGTGAATATCCCAATCTGGTGACGATTTACAGAAGCTTCAAACGTGTTGGTGCTTCCTATGA
  ATGGCAGAGCAGAGACACACCAATCCTACTTGAAGCCCCAACTGGCCTAGCAGAAGGAAGCTCCCTCCAT
  ACCCCACTGGCGCTGCCCCCACCACAACCCCCTCAACCAAGCTGTTCACCCTGTGCGCCAAGAGTCAGTG
  AGCCTGGAACATCCTCCCAGCAAAGCGATGAGATCCTGAGTGAGTCGCCCAGCCCATCTGACCCTGTCCT
  GCCTCTCCCTGCACTCATCCAGGAAGGAAGAAGCACTTCAGTGACCAATGACAAGTTAACATCCAAAATG
  AATGCGGAAGAAGACTCAGAAGAGATGCCCAGCCTCCTCACTAGCACTGTGCAAGTGGCCAGTGACAACC
  TGATCCCCCAAATAAGAGATAAAGAAGACCCTCAAGAGATGCCCCACTCTCCCTTGGGCTCTATGCCAGA
  GATAAGAGATAATTCTCCAGAACCAAATGACCCAGAAGAGCCCCAGGAGGTGTCCAGCACACCTTCAGAC
  AAGAAAGGAAAGAAAAGAAAAAGATGTATCTGGTCAACTCCAAAAAGGAGACATAAGAAAAAAAGCCTCC
  CAGGAGGGACAGCCTCATCTAGACACGGAATCCAAAAGAAGCTCAAAAGGGTGGATCAGGTTCCTCAAAA
  GAAAGATGACTCAACTTGTAACTCCACGGTAGAGACAAGGGCCCAAAAGGCGAGAACTGAATGTGCCCGA
  AAGTCGAGATCAGAGGAGATCATTGATGGCACTTCAGAAATGAATGAAGGAAAGAGGTCCCAGAAGACGC
  CTAGTACACCACGAAGGGTCACACAAGGGGCAGCCTCACCTGGGCATGGCATCCAAGAGAAGCTCCAAGT
  GGTGGATAAGGTGACTCAAAGGAAAGACGACTCAACCTGGAACTCAGAGGTCATGATGAGGGTCCAAAAG
  GCAAGAACTAAATGTGCCCGAAAGTCCAGATTGAAAGAAAAGAAAAAGGAGAAAGATATCTGTTCAAGCT
  CAAAAAGGAGATTTCAGAAAAATATTCACCGAAGAGGAAAACCCAAAAGTGACACTGTGGATTTTCACTG
  TTCTAAGCTCCCCGTGACCTGTGGTGAGGCGAAAGGGATTTTATATAAGAAGAAAATGAAACACGGATCC
  TCAGTGAAGTGCATTCGGAATGAGGATGGAACTTGGTTAACACCAAATGAATTTGAAGTCGAAGGAAAAG
  GAAGGAACGCAAAGAACTGGAAACGGAATATACGTTGTGAAGGAATGACCCTAGGAGAGCTGCTGAAGAG
  TGGACTTTTGCTCTGTCCTCCAAGAATAAATCTCAAGAGAGAGTTAAATAGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230264 representing NM_001185015
  Red=Cloning site Green=Tags(s)
  
  MGRGFRMFTMTRAMEEALFQHFMHQKLGIAYAIHKPFPFFEGLLDNSIITKRMYMESLEACRNLIPVSRV
  VHNILTQLERTFNLSLLVTLFSQINLREYPNLVTIYRSFKRVGASYEWQSRDTPILLEAPTGLAEGSSLH
  TPLALPPPQPPQPSCSPCAPRVSEPGTSSQQSDEILSESPSPSDPVLPLPALIQEGRSTSVTNDKLTSKM
  NAEEDSEEMPSLLTSTVQVASDNLIPQIRDKEDPQEMPHSPLGSMPEIRDNSPEPNDPEEPQEVSSTPSD
  KKGKKRKRCIWSTPKRRHKKKSLPGGTASSRHGIQKKLKRVDQVPQKKDDSTCNSTVETRAQKARTECAR
  KSRSEEIIDGTSEMNEGKRSQKTPSTPRRVTQGAASPGHGIQEKLQVVDKVTQRKDDSTWNSEVMMRVQK
  ARTKCARKSRLKEKKKEKDICSSSKRRFQKNIHRRGKPKSDTVDFHCSKLPVTCGEAKGILYKKKMKHGS
  SVKCIRNEDGTWLTPNEFEVEGKGRNAKNWKRNIRCEGMTLGELLKSGLLLCPPRINLKRELNSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001185015
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001185015.2
• RefSeq ORF: 1668 bp
• Locus ID: 3431
• UniProt ID: Q9HB58
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 63.1 kDa
• Gene Summary: The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. This gene is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Jul 2008]