MCT7 (SLC16A6) (NM_001174166) Human Tagged ORF Clone

CAT#: RC230227

SLC16A6 (Myc-DDK-tagged)-Human solute carrier family 16, member 6 (monocarboxylic acid transporter 7) (SLC16A6), transcript variant 1

Product Images

Circular map for RC230227

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  Western blot validation of overexpression lysate (Cat# LY433227) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230227 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MCT7
• Synonyms: MCT6; MCT7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230227 representing NM_001174166
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCAAAATAAATTAAAGCTTTGTTCCAAAGCCAATGTGTATACTGAAGTGCCTGATGGAGGATGGG
  GCTGGGCGGTAGCTGTTTCATTTTTCTTCGTTGAAGTCTTCACCTACGGCATCATCAAGACATTTGGTGT
  CTTCTTTAATGACTTAATGGACAGTTTTAATGAATCCAATAGCAGGATCTCATGGATAATCTCAATCTGT
  GTGTTTGTCTTAACATTTTCAGCTCCCCTCGCCACAGTCCTGAGCAATCGTTTCGGACACCGTCTGGTAG
  TGATGTTGGGGGGGCTACTTGTCAGCACCGGGATGGTGGCCGCCTCCTTCTCACAAGAGGTTTCTCATAT
  GTACGTCGCCATCGGCATCATCTCTGGTCTGGGATACTGCTTTAGTTTTCTCCCAACTGTAACCATCCTA
  TCACAATATTTTGGCAAAAGACGTTCCATAGTCACTGCAGTTGCTTCCACAGGAGAATGTTTCGCTGTGT
  TTGCTTTCGCACCAGCAATCATGGCTCTGAAGGAGCGCATTGGCTGGAGATACAGCCTCCTCTTCGTGGG
  CCTACTACAGTTAAACATTGTCATCTTCGGAGCACTGCTCAGACCCATCTTTATCAGAGGACCAGCGTCA
  CCGAAAATAGTCATCCAGGAAAATCGGAAAGAAGCGCAGTATATGCTTGAAAATGAGAAAACACGAACCT
  CAATAGACTCCATTGACTCAGGAGTAGAACTAACTACCTCACCTAAAAATGTGCCTACTCACACTAACCT
  GGAACTGGAGCCGAAGGCCGACATGCAGCAGGTCCTGGTGAAGACCAGCCCCAGGCCAAGCGAAAAGAAA
  GCCCCGCTATTAGACTTCTCCATTTTGAAAGAGAAAAGTTTTATTTGTTATGCATTATTTGGTCTCTTTG
  CAACACTGGGATTCTTTGCACCTTCCTTGTACATCATTCCTCTGGGCATTAGTCTGGGCATTGACCAGGA
  CCGCGCTGCTTTTTTATTATCTACGATGGCCATTGCAGAAGTTTTCGGAAGGATCGGAGCTGGTTTTGTC
  CTCAACAGGGAGCCCATTCGTAAGATTTACATTGAGCTCATCTGCGTCATCTTATTGACTGTGTCTCTGT
  TTGCCTTTACTTTTGCTACTGAATTCTGGGGTCTAATGTCATGCAGCATATTTTTTGGGTTTATGGTTGG
  AACAATAGGAGGGACTCACATTCCACTGCTTGCTGAGGATGATGTCGTGGGCATTGAGAAGATGTCTTCT
  GCAGCTGGGGTCTACATCTTCATTCAGAGCATAGCAGGACTGGCTGGACCGCCCCTTGCAGGTTTGTTGG
  TGGACCAAAGTAAGATCTACAGCAGGGCCTTCTACTCCTGCGCAGCTGGCATGGCCCTGGCTGCTGTGTG
  CCTCGCCCTGGTGAGACCGTGTAAGATGGGACTGTGCCAGCATCATCACTCAGGTGAAACAAAGGTAGTG
  AGCCATCGTGGGAAGACTTTACAGGACATACCTGAAGACTTTCTGGAAATGGATCTTGCAAAAAATGAGC
  ACAGAGTTCACGTGCAAATGGAGCCGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230227 representing NM_001174166
  Red=Cloning site Green=Tags(s)
  
  MTQNKLKLCSKANVYTEVPDGGWGWAVAVSFFFVEVFTYGIIKTFGVFFNDLMDSFNESNSRISWIISIC
  VFVLTFSAPLATVLSNRFGHRLVVMLGGLLVSTGMVAASFSQEVSHMYVAIGIISGLGYCFSFLPTVTIL
  SQYFGKRRSIVTAVASTGECFAVFAFAPAIMALKERIGWRYSLLFVGLLQLNIVIFGALLRPIFIRGPAS
  PKIVIQENRKEAQYMLENEKTRTSIDSIDSGVELTTSPKNVPTHTNLELEPKADMQQVLVKTSPRPSEKK
  APLLDFSILKEKSFICYALFGLFATLGFFAPSLYIIPLGISLGIDQDRAAFLLSTMAIAEVFGRIGAGFV
  LNREPIRKIYIELICVILLTVSLFAFTFATEFWGLMSCSIFFGFMVGTIGGTHIPLLAEDDVVGIEKMSS
  AAGVYIFIQSIAGLAGPPLAGLLVDQSKIYSRAFYSCAAGMALAAVCLALVRPCKMGLCQHHHSGETKVV
  SHRGKTLQDIPEDFLEMDLAKNEHRVHVQMEPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001174166
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174166.2
• RefSeq ORF: 1572 bp
• Locus ID: 9120
• UniProt ID: O15403
• Cytogenetics: 17q24.2
• Protein Families: Transmembrane
• MW: 57.8 kDa
• Gene Summary: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity).[UniProtKB/Swiss-Prot Function]