BANP (NM_001173542) Human Tagged ORF Clone

CAT#: RC230201

BANP (Myc-DDK-tagged)-Human BTG3 associated nuclear protein (BANP), transcript variant 6

Product Images

Circular map for RC230201

• 

  Western blot validation of overexpression lysate (Cat# LY433201) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230201 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BANP
• Synonyms: BEND1; SMAR1; SMARBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230201 representing NM_001173542
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCGGAACACGACCTGGCCGATGTGGTTCAGATTGCAGTGGAAGACCTGAGCCCTGACCACCCAG
  TTGTTTTGGAGAATCATGTAGTGACAGATGAAGACGAACCTGCTTTGAAACGCCAGCGACTAGAAATCAA
  TTGCCAGGATCCATCTATAAAGTCATTCCTGTATTCCATCAACCAGACAATCTGCTTGCGGTTGGATAGC
  ATTGAAGCCAAATTGCAAGCCCTGGAGGCTACTTGTAAATCCTTAGAAGAAAAGCTGGATCTGGTCACGA
  ACAAGCAGCACAGCCCCATCCAGGTCCCCATGGTGGCCGGCTCCCCTCTCGGGGCAACCCAGACGTGCAA
  CAAAGTGCGATGCGTCGTCCCCCAGACTACAGTAATACTCAACAATGATCGGCAGAACGCCATTGTAGCC
  AAGATGGAAGACCCCTTGAGCAACAGGGCACCGGATTCCCTGGAAAATGTCATTAGCAACGCTGTGCCTG
  GGCGTCGGCAGAACACCATTGTGGTGAAGGTGCCGGGCCAAGAAGACAGCCACCACGAGGACGGGGAGAG
  CGGCTCGGAGGCCAGCGACTCTGTGTCCAGCTGTGGGCAGGCGGGCAGTCAGAGCATCGGGAGCAACGTC
  ACGCTCATCACCCTGAACTCGGAAGAGGACTACCCCAATGGCACCTGGCTGGGCGACGAGAACAACCCCG
  AGATGCGGGTACGCTGCGCCATCATCCCCTCCGACATGCTGCACATCAGCACCAACTGCCGCACGGCCGA
  GAAGATGGCGCTCACGCTGCTGGACTACCTCTTCCACCGCGAGGTGCAGGCTGTGTCCAACCTCTCGGGG
  CAGGGCAAGCACGGGAAGAAGCAGCTGGACCCGCTCACCATCTACGGCATCCGGTGTCACCTTTTCTATA
  AATTTGGCATCACAGAATCCGACTGGTACCGAATCAAGCAGAGCATCGACTCCAAGTGCCGCACGGCGTG
  GCGGCGCAAGCAGCGGGGCCAGAGCCTGGCGGTCAAGAGCTTCTCGCGGAGAACGCCCAACTCGTCCTCC
  TACTGCCCTTCAGAGCCGATGATGAGCACCCCACCTCCTGCCAGCGAGCTCCCGCAGCCACAGCCGCAGC
  CGCAGGCCCTGCACTACGCGCTGGCCAACGCACAGCAGGTGCAGATCCACCAGATCGGAGAAGACGGACA
  GGTGCAAGTAATCCCACAGGGACACCTCCACATCGCCCAGGTGCCGCAGGGGGAGCAAGTCCAGATCACG
  CAGGACAGCGAGGGCAACCTCCAGATCCATCACGTGGGGCAGGACGGTCAGGTGCTGCAGGGTGCACAGC
  TGATCGCCGTGGCCTCCTCGGACCCCGCGGCGGCGGGCGTGGATGGGTCGCCACTCCAGGGCAGCGACAT
  CCAGGTTCAGTACGTGCAGCTGGCGCCAGTGAGTGACCACACGGCCGGGGCACAGACGGCCGAAGCCCTG
  CAGCCCACGCTACAGCCGGAGATGCAGCTCGAGCACGGGGCCATCCAGATTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230201 representing NM_001173542
  Red=Cloning site Green=Tags(s)
  
  MMSEHDLADVVQIAVEDLSPDHPVVLENHVVTDEDEPALKRQRLEINCQDPSIKSFLYSINQTICLRLDS
  IEAKLQALEATCKSLEEKLDLVTNKQHSPIQVPMVAGSPLGATQTCNKVRCVVPQTTVILNNDRQNAIVA
  KMEDPLSNRAPDSLENVISNAVPGRRQNTIVVKVPGQEDSHHEDGESGSEASDSVSSCGQAGSQSIGSNV
  TLITLNSEEDYPNGTWLGDENNPEMRVRCAIIPSDMLHISTNCRTAEKMALTLLDYLFHREVQAVSNLSG
  QGKHGKKQLDPLTIYGIRCHLFYKFGITESDWYRIKQSIDSKCRTAWRRKQRGQSLAVKSFSRRTPNSSS
  YCPSEPMMSTPPPASELPQPQPQPQALHYALANAQQVQIHQIGEDGQVQVIPQGHLHIAQVPQGEQVQIT
  QDSEGNLQIHHVGQDGQVLQGAQLIAVASSDPAAAGVDGSPLQGSDIQVQYVQLAPVSDHTAGAQTAEAL
  QPTLQPEMQLEHGAIQIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001173542
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001173542.1, NP_001167013.1
• RefSeq ORF: 1527 bp
• Locus ID: 54971
• UniProt ID: Q8N9N5
• Cytogenetics: 16q24.2
• MW: 56 kDa
• Gene Summary: This gene encodes a protein that binds to matrix attachment regions. The protein forms a complex with p53 and negatively regulates p53 transcription, and functions as a tumor suppressor and cell cycle regulator. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]