KLHDC4 (NM_001184854) Human Tagged ORF Clone

CAT#: RC230128

KLHDC4 (Myc-DDK-tagged)-Human kelch domain containing 4 (KLHDC4), transcript variant 3

Product Images

Circular map for RC230128

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLHDC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230128 representing NM_001184854
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGAAGGGCAAGAAGGAGAAGAAGGGCCGCGGCGCGGAGAAGACGGCCGCCAAGATGGAGAAGA
  AGGTGTCTAAGCGCTCGCGGAAGGAGGAGACTTTTTTGTATAACGAGCTCTATGTCTACAATACCAGAAA
  GGACACCTGGACCAAAGTTGACATCCCCAGTCCACCTCCGAGGCGCTGTGCTCACCAGGCGGTGGTAGTG
  CCTCAAGGTGGCGGACAGCTGTGGGTCTTTGGAGGGGAGTTTGCCTCTCCCAACGGAGAGCAGTTCTACC
  ACTACAAGGATCTCTGGGTCCTGCATTTGGCCACCAAGACCTGGGAACAAGTCAAATCAACAGGCGGTCC
  TTCGGGTCGGAGTGGACATCGGATGGTGGCCTGGAAGAGACAATTGATCCTGTTTGGTGGCTTCCATGAA
  AGTACACGGGATTACATCTACTACAACGACGTGTATGCCTTTAATCTGGACACCTTCACATGGAGCAAGC
  TGTCCCCGTCAGGGACGGGGCCCACACCCAGATCAGGCTGCCAGATGTCCGTCACTCCCCAGGGCGGCAT
  CGTCGTCTATGGGGGCTACTCGAAACAGAGAGTTAAGAAAGACGTGGACAAGGGCACACGGCACTCAGAC
  ATGTTCCTGCTGAAGCCAGAGGACGGAAGAGAAGACAAGTGGGTTTGGACTCGGATGAACCCTTCGGGGG
  TCAAGCCCACCCCACGGTCTGGCTTTTCCGTGGCCATGGCCCCGAATCACCAGACACTGTTCTTCGGGGG
  TGTCTGTGACGAGGAAGAGGAGGAGAGCCTGTCGGGCGAGTTCTTCAACGATCTGTACTTCTACGACGCC
  ACCAGGAACCGTTGGTTTGAGGGACAGCTGAAGGGACCCAAGTCTGAAAAGAAGAAACGCAGGCGGGGCA
  GAAAAGAGGAGCCCGAAGGTGGTAGCAGGCCGGCGTGTGGGGGAGCTGGCACCCAGGGGCCTGTGCAGCT
  GGTCAAGGAGGTGGTGGCCGAGGATGGCACCGTGGTCACCATTAAGCAGGTGCTCACCGCGCCAGGCTCG
  GCGGGGCAGCCCCGGTCTGAGGACGAAGACAGCCTTGAGGAGGCCGGCAGCCCCGCACCTGGGCCGTGTC
  CACGCTCCAACGCCATGCTGGCTGTGAAGCATGGGGTGCTCTACGTCTATGGGGGCATGTTTGAGGCCGG
  CGACCGCCAGGTCACCCTCAGCGACCTGCACTGCCTGGACCTGCACAGGATGGAGGCGTGGAAGGCCTTG
  GTGGAGATGGACCCAGAAACTCAGGAGTGGCTGGAGGAGACGGACTCGGAAGAGGACAGTGAGGAGGTTG
  AGGGCGCCGAGGGTGGGGTCGACGACGAAGACAGCGGAGAGGAGAGCGGTGCGGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230128 representing NM_001184854
  Red=Cloning site Green=Tags(s)
  
  MGKKGKKEKKGRGAEKTAAKMEKKVSKRSRKEETFLYNELYVYNTRKDTWTKVDIPSPPPRRCAHQAVVV
  PQGGGQLWVFGGEFASPNGEQFYHYKDLWVLHLATKTWEQVKSTGGPSGRSGHRMVAWKRQLILFGGFHE
  STRDYIYYNDVYAFNLDTFTWSKLSPSGTGPTPRSGCQMSVTPQGGIVVYGGYSKQRVKKDVDKGTRHSD
  MFLLKPEDGREDKWVWTRMNPSGVKPTPRSGFSVAMAPNHQTLFFGGVCDEEEEESLSGEFFNDLYFYDA
  TRNRWFEGQLKGPKSEKKKRRRGRKEEPEGGSRPACGGAGTQGPVQLVKEVVAEDGTVVTIKQVLTAPGS
  AGQPRSEDEDSLEEAGSPAPGPCPRSNAMLAVKHGVLYVYGGMFEAGDRQVTLSDLHCLDLHRMEAWKAL
  VEMDPETQEWLEETDSEEDSEEVEGAEGGVDDEDSGEESGAED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184854
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184854.2
• RefSeq ORF: 1392 bp
• Locus ID: 54758
• UniProt ID: Q8TBB5
• Cytogenetics: 16q24.2
• MW: 52 kDa