ASMT (NM_001171038) Human Tagged ORF Clone

CAT#: RC229958

ASMT (Myc-DDK-tagged)-Human acetylserotonin O-methyltransferase (ASMT), transcript variant 1

Product Images

Circular map for RC229958

• 

  Western blot validation of overexpression lysate (Cat# LY432958) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229958 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASMT
• Synonyms: ASMTY; HIOMT; HIOMTY
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229958 representing NM_001171038
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATCCTCAGAGGACCAGGCCTATCGCCTCCTTAATGACTACGCCAACGGCTTCATGGTGTCCCAGG
  TTCTCTTCGCCGCCTGCGAGCTGGGCGTGTTTGACCTTCTCGCCGAGGCCCCAGGGCCCCTGGACGTGGC
  GGCAGTGGCTGCAGGTGTGAGGGCCAGCGCCCATGGGACAGAGCTCCTGCTGGACATCTGTGTGTCCCTG
  AAGCTGCTGAAAGTGGAGACGAGGGGAGGAAAAGCTTTCTATCGAAACACAGAGCTGTCCAGCGACTACC
  TGACCACGGTCAGCCCGACGTCACAATGCAGCATGCTGAAGTACATGGGCAGGACCAGCTACCGGTGCTG
  GGGCCACCTGGCAGACGCCGTGAGAGAAGGAAGGAACCAGTACCTGGAGACGTTTGGCGTTCCCGCTGAA
  GAGCTTTTTACGGCCATCTACAGGTCCGAGGGCGAGCGGCTACAGTTCATGCAAGCTCTGCAGGAGGTCT
  GGAGCGTCAACGGGAGAAGCGTGCTGACCGCCTTTGACCTGTCAGTGTTCCCACTTATGTGTGACCTTGG
  TGGGACATGGATAAAGCTGGAAACCATCATTCTCAGCAAACTATCGCAAGGACAGAAAACCAAACACCGC
  GTGTTCTCACTCATAGGTGGGGCTGGAGCTCTGGCTAAGGAATGCATGTCTCTGTACCCTGGATGTAAGA
  TCACCGTTTTTGACATCCCAGAAGTGGTGTGGACGGCAAAGCAGCACTTCTCATTCCAGGAGGAAGAACA
  GATTGACTTCCAGGAAGGGGATTTCTTCAAAGACCCTCTTCCGGAAGCTGATCTGTACATCCTGGCCAGG
  GTCCTCCATGACTGGGCAGACGGAAAGTGCTCACACCTGCTGGAGAGGATCTACCACACTTGCAAGCCAG
  GTGGTGGCATTCTGGTAATTGAAAGCCTCCTGGATGAAGACAGGCGAGGTCCTCTGCTCACGCAGCTCTA
  CTCTCTGAACATGCTTGTGCAGACGGAAGGGCAGGAGAGGACCCCCACCCACTACCACATGCTCCTCTCT
  TCTGCTGGCTTCAGAGACTTCCAGTTTAAGAAAACAGGAGCCATTTATGATGCCATTTTAGCCAGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229958 representing NM_001171038
  Red=Cloning site Green=Tags(s)
  
  MGSSEDQAYRLLNDYANGFMVSQVLFAACELGVFDLLAEAPGPLDVAAVAAGVRASAHGTELLLDICVSL
  KLLKVETRGGKAFYRNTELSSDYLTTVSPTSQCSMLKYMGRTSYRCWGHLADAVREGRNQYLETFGVPAE
  ELFTAIYRSEGERLQFMQALQEVWSVNGRSVLTAFDLSVFPLMCDLGGTWIKLETIILSKLSQGQKTKHR
  VFSLIGGAGALAKECMSLYPGCKITVFDIPEVVWTAKQHFSFQEEEQIDFQEGDFFKDPLPEADLYILAR
  VLHDWADGKCSHLLERIYHTCKPGGGILVIESLLDEDRRGPLLTQLYSLNMLVQTEGQERTPTHYHMLLS
  SAGFRDFQFKKTGAIYDAILARK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001171038
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001171038.2
• RefSeq ORF: 1122 bp
• Locus ID: 438
• UniProt ID: P46597
• Cytogenetics: X;Y
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tryptophan metabolism
• MW: 42.1 kDa
• Gene Summary: This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2010]