ATP13A1 (NM_020410) Human Tagged ORF Clone

CAT#: RC229417

ATP13A1 (Myc-DDK-tagged)-Human ATPase type 13A1 (ATP13A1)

Product Images

Circular map for RC229417

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATP13A1
• Synonyms: ATP13A; CGI-152
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229417 representing NM_020410
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGCGGCGGCGGTGGGCAACGCGGTGCCCTGCGGGGCCCGGCCTTGCGGGGTCCGGCCTGACG
  GGCAGCCCAAGCCCGGGCCGCAGCCGCGCGCGCTCCTTGCCGCCGGGCCGGCGCTCATAGCGAACGGTGA
  CGAGCTGGTGGCTGCCGTGTGGCCGTACCGGCGGTTGGCGCTGTTGCGGCGCCTCACGGTGCTGCCATTC
  GCCGGGCTGCTTTACCCGGCCTGGTTGGGTGCCGCAGCCGCTGGCTGCTGGGGCTGGGGCAGCAGTTGGG
  TGCAGATCCCCGAAGCTGCGCTGCTCGTGCTTGCCACCATCTGCCTCGCGCACGCGCTCACTGTCCTCTC
  GGGGCATTGGTCTGTGCACGCGCATTGCGCGCTCACCTGCACCCCGGAGTACGACCCCAGCAAAGCGACC
  TTTGTGAAGGTGGTGCCAACCCCCAACAATGGCTCCACGGAGCTCGTGGCCCTGCACCGCAATGAGGGCG
  AAGACGGGCTTGAGGTGCTGTCCTTCGAATTCCAGAAGATCAAGTATTCCTACGATGCCCTGGAGAAGAA
  GCAGTTTCTCCCCGTGGCCTTTCCTGTGGGAAACGCCTTCTCATACTATCAGAGCAACAGAGGCTTCCAG
  GAAGACTCAGAGATCCGAGCAGCTGAGAAGAAATTTGGGAGCAACAAGGCCGAGATGGTGGTGCCTGACT
  TCTCGGAGCTTTTCAAGGAGAGAGCCACAGCCCCCTTCTTTGTATTTCAGGTGTTCTGTGTGGGGCTCTG
  GTGCCTGGATGAGTACTGGTACTACAGCGTCTTTACGCTATCCATGCTGGTGGCGTTCGAGGCCTCGCTG
  GTGCAGCAGCAGATGCGGAACATGTCGGAGATCCGGAAGATGGGCAACAAGCCCCACATGATCCAGGTCT
  ACCGAAGCCGCAAGTGGAGGCCCATTGCCAGTGATGAGATCGTACCAGGGGACATCGTCTCCATCGGCCG
  CTCCCCACAGGAGAACCTGGTGCCATGTGACGTGCTTCTGCTGCGAGGCCGCTGCATCGTAGACGAGGCC
  ATGCTCACGGGGGAGTCCGTGCCACAGATGAAGGAGCCCATCGAAGACCTCAGCCCAGACCGGGTGCTGG
  ACCTCCAGGCTGATTCCCGGCTGCACGTCATCTTCGGGGGCACCAAGGTGGTGCAGCACATCCCCCCACA
  GAAAGCCACCACGGGCCTGAAGCCGGTTGACAGCGGGTGCGTGGCCTACGTCCTGCGGACCGGATTCAAC
  ACATCCCAGGGCAAGCTGCTGCGCACCATCCTCTTCGGGGTCAAGAGGGTGACTGCGAACAACCTGGAGA
  CCTTCATCTTCATCCTCTTCCTCCTGGTGTTTGCCATCGCTGCAGCTGCCTATGTATGGATTGAAGGTAC
  CAAGGACCCCAGCCGGAACCGCTACAAGCTGTTTCTGGAGTGCACCCTGATCCTCACCTCGGTCGTGCCT
  CCTGAGCTGCCCATCGAGCTGTCCCTGGCCGTCAACACCTCCCTCATCGCCCTGGCCAAGCTCTACATGT
  ACTGCACAGAGCCCTTCCGGATCCCCTTTGCTGGCAAGGTCGAGGTGTGCTGCTTTGACAAGACGGGGAC
  GTTGACCAGTGACAGCCTGGTGGTGCGCGGTGTGGCCGGGCTGAGAGACGGGAAGGAGGTGACCCCAGTG
  TCCAGCATCCCTGTAGAAACACACCGGGCCCTGGCCTCGTGCCACTCGCTCATGCAGCTGGACGACGGCA
  CCCTCGTGGGTGACCCTCTAGAGAAGGCCATGCTGACGGCCGTGGACTGGACGCTGACCAAAGATGAGAA
  AGTATTCCCCCGAAGTATTAAAACTCAGGGGCTGAAAATTCACCAGCGCTTTCATTTTGCCAGTGCCCTG
  AAGCGAATGTCCGTGCTTGCCTCGTATGAGAAGCTGGGCTCCACCGACCTCTGCTACATCGCGGCCGTGA
  AGGGGGCCCCCGAAACTCTGCACTCCATGTTCTCCCAGTGCCCGCCCGACTACCACCACATCCACACCGA
  GATCTCCCGGGAAGGAGCCCGCGTCCTGGCGCTGGGGTACAAGGAGCTGGGACACCTCACTCACCAGCAG
  GCCCGGGAGGTCAAGCGGGAGGCCCTGGAGTGCAGCCTCAAGTTCGTCGGCTTCATTGTGGTCTCCTGCC
  CGCTCAAGGCTGACTCCAAGGCCGTGATCCGGGAGATCCAGAATGCGTCCCACCGGGTGGTCATGATCAC
  GGGAGACAACCCGCTCACTGCATGCCACGTGGCCCAGGAGCTGCACTTCATTGAAAAGGCCCACACGCTG
  ATCCTGCAGCCTCCCTCCGAGAAAGGCCGGCAGTGCGAGTGGCGCTCCATTGACGGCAGCATCGTGCTGC
  CCCTGGCCCGGGGCTCCCCAAAGGCACTGGCCCTGGAGTACGCACTGTGCCTCACAGGCGACGGCTTGGC
  CCACCTGCAGGCCACCGACCCCCAGCAGCTGCTCCGCCTCATCCCCCATGTGCAGGTGTTCGCCCGTGTG
  GCTCCCAAGCAGAAGGAGTTTGTCATCACCAGCCTGAAGGAGCTGGGCTACGTGACCCTCATGTGTGGGG
  ATGGCACCAACGACGTGGGCGCCCTGAAGCATGCTGACGTGGGTGTGGCGCTCTTGGCCAATGCCCCTGA
  GCGGGTTGTCGAGCGGCGACGGCGGCCCCGGGACAGCCCAACCCTGAGCAACAGTGGCATCAGAGCCACC
  TCCAGGACAGCCAAGCAGCGGTCGGGGCTCCCTCCCTCCGAGGAGCAGCCAACCTCCCAGAGGGACCGCC
  TGAGCCAGGTGCTGCGAGACCTCGAGGACGAGAGTACGCCCATTGTGAAACTGGGGGATGCCAGCATCGC
  AGCACCCTTCACCTCCAAGCTCTCATCCATCCAGTGCATCTGCCACGTGATCAAGCAGGGCCGCTGCACG
  CTGGTGACCACGCTACAGATGTTCAAGATCCTGGCGCTCAATGCCCTCATCCTGGCCTACAGCCAGAGCG
  TCCTCTACCTGGAGGGAGTCAAGTTCAGTGACTTCCAGGCCACCCTACAGGGGCTGCTGCTGGCCGGCTG
  CTTCCTCTTCATCTCCCGTTCCAAGCCCCTCAAGACCCTCTCCCGAGAACGGCCCCTGCCCAACATCTTC
  AACCTGTACACCATCCTCACCGTCATGCTCCAGTTCTTTGTGCACTTCCTGAGCCTTGTCTACCTGTACC
  GTGAGGCCCAGGCCCGGAGCCCCGAGAAGCAGGAGCAGTTCGTGGACTTGTACAAGGAGTTTGAGCCAAG
  CCTGGTCAACAGCACCGTCTACATCATGGCCATGGCCATGCAGATGGCCACCTTCGCCATCAATTACAAA
  GGCCCGCCCTTCATGGAGAGCCTGCCCGAGAACAAGCCCCTGGTGTGGAGTCTGGCAGTTTCACTCCTGG
  CCATCATTGGCCTGCTCCTCGGCTCCTCGCCCGACTTCAACAGCCAGTTTGGCCTCGTGGACATCCCTGT
  GGAGTTCAAGCTGGTCATTGCCCAGGTCCTGCTCCTGGACTTCTGCCTGGCGCTCCTGGCCGACCGCGTC
  CTGCAGTTCTTCCTGGGGACCCCGAAGCTGAAAGTGCCTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229417 representing NM_020410
  Red=Cloning site Green=Tags(s)
  
  MAAAAAVGNAVPCGARPCGVRPDGQPKPGPQPRALLAAGPALIANGDELVAAVWPYRRLALLRRLTVLPF
  AGLLYPAWLGAAAAGCWGWGSSWVQIPEAALLVLATICLAHALTVLSGHWSVHAHCALTCTPEYDPSKAT
  FVKVVPTPNNGSTELVALHRNEGEDGLEVLSFEFQKIKYSYDALEKKQFLPVAFPVGNAFSYYQSNRGFQ
  EDSEIRAAEKKFGSNKAEMVVPDFSELFKERATAPFFVFQVFCVGLWCLDEYWYYSVFTLSMLVAFEASL
  VQQQMRNMSEIRKMGNKPHMIQVYRSRKWRPIASDEIVPGDIVSIGRSPQENLVPCDVLLLRGRCIVDEA
  MLTGESVPQMKEPIEDLSPDRVLDLQADSRLHVIFGGTKVVQHIPPQKATTGLKPVDSGCVAYVLRTGFN
  TSQGKLLRTILFGVKRVTANNLETFIFILFLLVFAIAAAAYVWIEGTKDPSRNRYKLFLECTLILTSVVP
  PELPIELSLAVNTSLIALAKLYMYCTEPFRIPFAGKVEVCCFDKTGTLTSDSLVVRGVAGLRDGKEVTPV
  SSIPVETHRALASCHSLMQLDDGTLVGDPLEKAMLTAVDWTLTKDEKVFPRSIKTQGLKIHQRFHFASAL
  KRMSVLASYEKLGSTDLCYIAAVKGAPETLHSMFSQCPPDYHHIHTEISREGARVLALGYKELGHLTHQQ
  AREVKREALECSLKFVGFIVVSCPLKADSKAVIREIQNASHRVVMITGDNPLTACHVAQELHFIEKAHTL
  ILQPPSEKGRQCEWRSIDGSIVLPLARGSPKALALEYALCLTGDGLAHLQATDPQQLLRLIPHVQVFARV
  APKQKEFVITSLKELGYVTLMCGDGTNDVGALKHADVGVALLANAPERVVERRRRPRDSPTLSNSGIRAT
  SRTAKQRSGLPPSEEQPTSQRDRLSQVLRDLEDESTPIVKLGDASIAAPFTSKLSSIQCICHVIKQGRCT
  LVTTLQMFKILALNALILAYSQSVLYLEGVKFSDFQATLQGLLLAGCFLFISRSKPLKTLSRERPLPNIF
  NLYTILTVMLQFFVHFLSLVYLYREAQARSPEKQEQFVDLYKEFEPSLVNSTVYIMAMAMQMATFAINYK
  GPPFMESLPENKPLVWSLAVSLLAIIGLLLGSSPDFNSQFGLVDIPVEFKLVIAQVLLLDFCLALLADRV
  LQFFLGTPKLKVPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020410
• ORF Size: 3612 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020410.3
• RefSeq ORF: 3615 bp
• Locus ID: 57130
• UniProt ID: Q9HD20
• Cytogenetics: 19p13.11
• Domains: E1-E2_ATPase, Hydrolase
• Protein Families: Transmembrane
• MW: 132.8 kDa
• Gene Summary: Mediates manganese transport into the endoplasmic reticulum. The ATPase activity is required for cellular manganese homeostasis.[UniProtKB/Swiss-Prot Function]