ZYG11A (NM_001004339) Human Tagged ORF Clone

CAT#: RC229364

ZYG11A (Myc-DDK-tagged)-Human zyg-11 homolog A (C. elegans) (ZYG11A)

Product Images

Circular map for RC229364

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZYG11A
• Synonyms: ZYG11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229364 representing NM_001004339
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCATTTCTTGCACCCGGGCCACACGCCCCGGAACATCGTCCCTCCTGACGCTCAGAAGGATGCCC
  TGGGCTGCTGCGTGGTACAGGAAGAGGCATCTCCCTACACTTTGGTCAACATCTGCCTGAATGTCCTGAT
  TGCTAACCTGGAGAAATTGTGTTCTGAAAGACCTGATGGAACACTGTGCCTTCCGGAGCATTGGAGTTTC
  CCTCAGGAAGTAGCCGAGCGATTTCTCAGGGTGATGACTTGGCAAGGCAAGCTGACTGACAGAACAGCCA
  GCATTTTCCGAGGCAACCAAATGAAACTGAAGCTGGTCAATATCCAAAAAGCTAAAATCTCTACAGCTGC
  ATTCATAAAAGCCTTCTGCCGTCATAAGCTCATTGAACTGAATGCTACTGCAGTGCACGCTGACCTCCCA
  GTTCCAGACATCATAAGTGGACTCTGCAGCAATAGGTGGATCCAGCAAAACCTCCAGTGTCTCCTGTTAG
  ACTCGACAAGCATCCCTCAAAATTCAAGATTACTGTTCTTTAGTCAGCTCACTGGTCTTCGCATTTTAAG
  TGTTTTTAATGTTTGTTTTCATACTGAAGACCTGGCTAATGTTTCTCAGTTACCAAGACTGGAAAGCTTA
  GATATCTCTAATACTCTAGTCACTGATATTTCTGCACTGCTTACCTGTAAGGATCGATTGAAGTCTCTCA
  CAATGCACTATCTGAAATGCCTGGCCATGACCAAATCACAAATTCTTGCAGTCATTAGAGAACTTAAATG
  TCTGCTTCACCTTGATATTTCTGATCACAGGCAACTCAAATCAGACCTAGCTTTTCATTTGCTACAGCAG
  AAGGATATCCTGCCCAATGTTGTGTCATTGGATATTTCTGGGGGCAATTGCATCACTGATGAAGCTGTAG
  AACTGTTTATACGACTGCGGCCTGCCATGCAATTTGTGGGACTATTGGCCACGGATGCTGGCTCTTCTGA
  CTTCTTTACTACAAAGCAAGGCTTGAGGGTTGCTGGAGGAGCCAGTATGAGTCAGATTTCAGAAGCACTG
  AGCCGATACAGGAACAGATCATGTTTTGTGAAGGAAGCCCTCCACAGGCTGTTCACAGAGACATTTTCAA
  TGGAGGTAACCATGCCTGCTATTTTAAAGCTTGTGGCTATAGGAATGAGGAATCACCCATTGGATTTGCG
  AGTGCAGTTCACAGCCAGTGCTTGCGCTCTCAACCTAACACGCCAGGGCCTGGCCAAGGGGATGCCTGTT
  CGCCTGTTGTCAGAGGTCACCTGTCTACTTTTCAAGGCTCTGAAAAATTTCCCCCATTACCAGCAGTTAC
  AGAAGAATTGTCTTCTCTCCTTAACCAATTCCAGGATTCTTGTGGATGTTCCATTTGACAGGTTTGATGC
  TGCCAAGTTTGTCATGAGATGGCTCTGTAAGCATGAAAACCCCAAGATGCAAACAATGGCAGTGAGTGTC
  ACCTCTATTCTGGCTCTGCAGCTCTCACCTGAGCAAACGGCACAGCTTGAAGAGCTTTTCATGGCAGTTA
  AGGAACTTCTAGCAATAGTAAAACAAAAGACTACTGAGAATTTAGATGATGTCACCTTCTTGTTTACTTT
  GAAAGCACTTTGGAATCTTACAGATGGGTCTCCAGCTGCCTGCAAGCACTTCATTGAAAATCAAGGATTG
  CAAATCTTCATCCAAGTCTTGGAGACCTTTTCAGAGTCAGCAATACAAAGCAAAGTACTTGGTCTTTTGA
  ACAACATAGCAGAAGTCAGAGAGCTCTCTTCCAAGCTGGTGACCGAAGATGTGCTGAAGCATATCAACAG
  TTTACTCTGTAGCAGGGAAATGGAAGTCAGCTATTTTGCTGCAGGTATCATAGCCCACCTGACATCTGAC
  AGACAGCTTTGGATATCCCGTGACTTCCAGAGGCGTACTCTTCTCCAAGATCTGCATGCAACCATACAGA
  ATTGGCCAAGTTCAAGTTGTAAGATGACAGCATTGGTGACCTATAGATCTTTCAAGACATTTTTCCCGCT
  CCTTGGCAACTTCTCTCAACCAGAGGTTCAGCTCTGGGCACTATGGGCTATGTATCATGTCTGCAGTAAA
  AATCCCAGCAAATACTGCAAAATGTTAGTTGAAGAAGAAGGATTGCAGCTTTTGTGTGATATCCAGGAGC
  ACAGTGAGGCAACCCCCAAAGCACAGCAGATTGCAGCCTCCATTCTGGATGACTTCAGAATGCATTTCAT
  GAATTATCAGAGGCCCACTCTGTGTCAAATGCCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229364 representing NM_001004339
  Red=Cloning site Green=Tags(s)
  
  MVHFLHPGHTPRNIVPPDAQKDALGCCVVQEEASPYTLVNICLNVLIANLEKLCSERPDGTLCLPEHWSF
  PQEVAERFLRVMTWQGKLTDRTASIFRGNQMKLKLVNIQKAKISTAAFIKAFCRHKLIELNATAVHADLP
  VPDIISGLCSNRWIQQNLQCLLLDSTSIPQNSRLLFFSQLTGLRILSVFNVCFHTEDLANVSQLPRLESL
  DISNTLVTDISALLTCKDRLKSLTMHYLKCLAMTKSQILAVIRELKCLLHLDISDHRQLKSDLAFHLLQQ
  KDILPNVVSLDISGGNCITDEAVELFIRLRPAMQFVGLLATDAGSSDFFTTKQGLRVAGGASMSQISEAL
  SRYRNRSCFVKEALHRLFTETFSMEVTMPAILKLVAIGMRNHPLDLRVQFTASACALNLTRQGLAKGMPV
  RLLSEVTCLLFKALKNFPHYQQLQKNCLLSLTNSRILVDVPFDRFDAAKFVMRWLCKHENPKMQTMAVSV
  TSILALQLSPEQTAQLEELFMAVKELLAIVKQKTTENLDDVTFLFTLKALWNLTDGSPAACKHFIENQGL
  QIFIQVLETFSESAIQSKVLGLLNNIAEVRELSSKLVTEDVLKHINSLLCSREMEVSYFAAGIIAHLTSD
  RQLWISRDFQRRTLLQDLHATIQNWPSSSCKMTALVTYRSFKTFFPLLGNFSQPEVQLWALWAMYHVCSK
  NPSKYCKMLVEEEGLQLLCDIQEHSEATPKAQQIAASILDDFRMHFMNYQRPTLCQMPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004339
• ORF Size: 2277 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004339.3
• RefSeq ORF: 2280 bp
• Locus ID: 440590
• UniProt ID: Q6WRX3
• Cytogenetics: 1p32.3
• MW: 85.6 kDa
• Gene Summary: Probably acts as target recruitment subunit in an E3 ubiquitin ligase complex ZYGA-CUL2-elongin BC.[UniProtKB/Swiss-Prot Function]