SH2B2 (NM_020979) Human Tagged ORF Clone

CAT#: RC229333

SH2B2 (Myc-DDK-tagged)-Human SH2B adaptor protein 2 (SH2B2)

Product Images

Circular map for RC229333

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  Western blot validation of overexpression lysate (Cat# LY432348) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229333 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SH2B2 protein (Cat# TP329333). The protein was produced from HEK293T cells transfected with SH2B2 cDNA clone (Cat# RC229333) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SH2B2
• Synonyms: APS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229333 representing NM_020979
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCAAGCTATTGTCCTGCCCATGGCTTCCCATCTCAGGACCCTCTCTGGCCGCTATCATCCCAGC
  AGTGGAGTTCAGCCCACTACTCTGAACCAGCCGCAGGTGGCTGCGATGGGACGGAAGCCATGAATGGTGC
  CGGCCCTGGCCCCGCCGCAGCCGCCCCGGTCCCAGTCCCGGTCCCGGTCCCGGACTGGCGGCAGTTCTGC
  GAGCTGCATGCGCAGGCGGCCGCCGTGGACTTTGCGCACAAGTTCTGCCGTTTCCTGCGGGACAACCCAG
  CTTACGACACGCCCGACGCCGGCGCCTCCTTCTCCCGCCACTTCGCCGCCAACTTCCTGGACGTCTTCGG
  CGAGGAGGTGCGCCGCGTGCTGGTGGCTGGGCCGACGACTCGGGGCGCGGCCGTGAGCGCAGAGGCCATG
  GAGCCGGAGCTCGCGGACACCTCTGCACTCAAGGCGGCGCCCTACGGCCACTCGCGGAGCTCGGAGGACG
  TGTCCACGCACGCGGCCACCAAGGCCCGCGTTCGCAAGGGCTTCTCGCTGCGCAACATGAGCCTGTGCGT
  GGTGGACGGCGTGCGCGACATGTGGCACCGGCGCGCCTCGCCCGAGCCCGACGCGGCAGCTGCCCCGCGC
  ACCGCCGAGCCCCGCGACAAGTGGACGCGGCGCCTGAGGCTGTCGCGGACGCTGGCTGCCAAGGTGGAGC
  TGGTGGACATTCAACGCGAGGGGGCGCTGCGCTTCATGGTGGCCGACGACGCGGCCGCGGGCTCCGGGGG
  CTCGGCTCAGTGGCAGAAGTGCCGCCTGCTCCTGCGCAGGGCTGTGGCCGAGGAACGCTTCCGCCTGGAG
  TTCTTCGTGCCGCCCAAAGCCTCCAGGCCCAAGGTCAGCATCCCACTGTCAGCCATCATTGAGGTCCGCA
  CCACCATGCCCCTGGAAATGCCAGAGAAGGATAACACATTCGTCCTCAAGGTAGAGAATGGAGCCGAATA
  CATCTTGGAGACCATCGACTCTCTGCAGAAGCACTCGTGGGTAGCTGACATCCAGGGCTGCGTGGACCCC
  GGTGACAGTGAGGAAGACACCGAGCTCTCCTGTACCCGAGGAGGCTGTCTGGCCAGCCGCGTGGCCTCCT
  GCAGCTGTGAGCTCCTGACTGATGCAGTCGACCTGCCCCGCCCCCCAGAGACGACAGCCGTGGGTGCAGT
  GGTGACAGCCCCCCACAGCCGAGGTCGAGATGCCGTCAGAGAATCCCTGATCCACGTCCCGCTAGAGACC
  TTTCTGCAGACCCTGGAATCCCCGGGCGGCAGCGGCAGTGACAGCAATAACACAGGGGAACAGGGTGCAG
  AGACGGATCCCGAGGCTGAACCCGAGCTGGAGCTATCCGACTACCCATGGTTCCACGGGACACTGTCCCG
  GGTCAAGGCTGCTCAACTGGTTCTGGCAGGGGGGCCCCGGAACCACGGCCTCTTCGTGATCCGCCAAAGT
  GAGACTCGGCCTGGGGAGTACGTGCTGACCTTCAACTTCCAGGGCAAGGCCAAGCACCTGCGCCTGTCCC
  TGAACGGCCACGGCCAGTGTCACGTACAGCATCTGTGGTTCCAGTCTGTGCTTGACATGCTCCGCCACTT
  CCACACACACCCCATCCCACTGGAGTCAGGGGGCTCGGCCGACATCACCCTTCGCAGCTATGTGCGGGCC
  CAGGACCCCCCACCAGAGCCGGGCCCCACGCCCCCTGCCGCGCCCGCGTCCCCGGCCTGCTGGAGCGACT
  CGCCCGGCCAGCACTACTTCTCCAGCCTCGCCGCGGCCGCCTGCCCGCCTGCCTCGCCCTCCGACGCCGC
  CGGCGCCTCCTCGTCTTCCGCCTCGTCGTCCTCTGCCGCGTCGGGGCCCGCCCCCCCGCGCCCCGTCGAG
  GGCCAGCTCAGCGCGCGGAGCCGCAGCAACAGCGCCGAGCGCCTGCTGGAGGCCGTGGCCGCCACCGCCG
  CCGAGGAGCCCCCGGAGGCCGCGCCCGGCCGCGCGCGCGCCGTGGAGAACCAGTACTCCTTCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229333 representing NM_020979
  Red=Cloning site Green=Tags(s)
  
  MDPSYCPAHGFPSQDPLWPLSSQQWSSAHYSEPAAGGCDGTEAMNGAGPGPAAAAPVPVPVPVPDWRQFC
  ELHAQAAAVDFAHKFCRFLRDNPAYDTPDAGASFSRHFAANFLDVFGEEVRRVLVAGPTTRGAAVSAEAM
  EPELADTSALKAAPYGHSRSSEDVSTHAATKARVRKGFSLRNMSLCVVDGVRDMWHRRASPEPDAAAAPR
  TAEPRDKWTRRLRLSRTLAAKVELVDIQREGALRFMVADDAAAGSGGSAQWQKCRLLLRRAVAEERFRLE
  FFVPPKASRPKVSIPLSAIIEVRTTMPLEMPEKDNTFVLKVENGAEYILETIDSLQKHSWVADIQGCVDP
  GDSEEDTELSCTRGGCLASRVASCSCELLTDAVDLPRPPETTAVGAVVTAPHSRGRDAVRESLIHVPLET
  FLQTLESPGGSGSDSNNTGEQGAETDPEAEPELELSDYPWFHGTLSRVKAAQLVLAGGPRNHGLFVIRQS
  ETRPGEYVLTFNFQGKAKHLRLSLNGHGQCHVQHLWFQSVLDMLRHFHTHPIPLESGGSADITLRSYVRA
  QDPPPEPGPTPPAAPASPACWSDSPGQHYFSSLAAAACPPASPSDAAGASSSSASSSSAASGPAPPRPVE
  GQLSARSRSNSAERLLEAVAATAAEEPPEAAPGRARAVENQYSFY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020979
• ORF Size: 2025 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020979.5
• RefSeq ORF: 2028 bp
• Locus ID: 10603
• UniProt ID: O14492
• Cytogenetics: 7q22.1
• Domains: SH2, PH
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, Neurotrophin signaling pathway
• MW: 72.1 kDa
• Gene Summary: The protein encoded by this gene is expressed in B lymphocytes and contains pleckstrin homology and src homology 2 (SH2) domains. In Burkitt's lymphoma cell lines, it is tyrosine-phosphorylated in response to B cell receptor stimulation. Because it binds Shc independent of stimulation and Grb2 after stimulation, it appears to play a role in signal transduction from the receptor to the Shc/Grb2 pathway. [provided by RefSeq, Jun 2009]