CCDC114 (NM_144577) Human Tagged ORF Clone

CAT#: RC229332

CCDC114 (Myc-DDK-tagged)-Human coiled-coil domain containing 114 (CCDC114), transcript variant 2

Product Images

Circular map for RC229332

• 

  Western blot validation of overexpression lysate (Cat# LY432347) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229332 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC114
• Synonyms: CILD20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229332 representing NM_144577
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGGAGAGGCGGGCCTACAGCAAGGAAGTCCACCAGCGCATCAACAAGCAACTTGAGGAGATCC
  GGCGCTTGGAGGAGGTACGGGGCGATCTCCAGGTGCAGATCAGCGCAGCCCAGAACCAGGTCAAGCGGCT
  TCGGGACAGTCAGCGGCTGGAGAACATGGACCGCCTGCTGAAGGGCCGGGCCCAGGTGCAGGCGGAGATC
  GAGGAGCTGCAGGAGCAGACCAGGGCCCTGGACAAGCAGATCCAGGAGTGGGAGACGCGGATCTTTACCC
  ACAGTAAGAATGTCAGGTCCCCGGGATTCATCCTGGATCAGAAGGTCAAGATCAGGCGAAGGATCAGGAT
  CCTAGAAAACCAGTTGGACAGGGTCACCTGTCACTTTGACAACCAGCTGGTACGGAATGCGGCCCTGCGG
  GAGGAGCTGGATCTGCTGCGGATCGACAGGAACCGCTATCTGAACGTGGACCGCAAGCTGAAGAAGGAGA
  TCCACCACCTGCATCACCTGGTCAGCACCCTTATCCTCTCCTCCACCTCTGCCTACGCCGTCAGGGAGGA
  GGCGAAGGCCAAGATGGGCTTGCTGCGGGAGCGCGCGGAGAAAGAGGAGGCCCAGAGCGAGATGGAGGCG
  CAGGTCCTGCAGCGGCAGATCTTGCACCTGGAGCAGCTGCACCACTTCCTCAAGCTCAAGAACAACGACC
  GGCAGCCGGATCCCGATGTCCTGGAGAAGCGTGAAAAGCAGGCCGGGGAGGTGGCCGAGGGCGTCTGGAA
  GACCTCCCAGGAGAGGCTGGTGCTTTGCTACGAGGACGCCCTGAATAAACTGTCCCAGCTGATGGGGGAG
  AGTGACCCTGACCTGTTGGTGCAGAAGTATCTGGAGATCGAGGAGCGCAACTTTGCTGAGTTCAACTTCA
  TCAACGAGCAGAACTTGGAGCTGGAGCATGTGCAGGAAGAGATCAAGGAGATGCAGGAGGCTTTGGTGAG
  CGCACGTGCCAGCAAGGATGACCAGCATTTGCTGCAGGAGCAGCAGCAGAAGGTGTTGCAGCAGCGCATG
  GACAAGGTGCACTCGGAGGCTGAGCGCCTTGAGGCCCGCTTCCAGGATGTGCGGGGACAGCTGGAGAAGC
  TCAAGGCTGATATCCAGCTCCTCTTCACCAAGGCCCATTGCGACAGCAGCATGATCGATGACCTCCTTGG
  GGTCAAGACCAGCATGGGAGACCGGGACATGGGCCTCTTCCTGAGCCTCATTGAGAAGCGGCTGGTGGAG
  CTCCTGACAGTGCAGGCCTTCCTACATGCCCAGAGCTTCACCTCCCTGGCCGACGCTGCCCTCCTAGTGC
  TGGGCCAGAGCCTGGAGGACCTTCCGAAGAAGATGGCCCCACTTCAGCCCCCTGACACTCTAGAAGACCC
  CCCGGGTTTTGAGGCCAGCGATGACTACCCCATGAGCAGGGAGGAGCTGCTGAGCCAAGTGGAGAAGCTG
  GTGGAGCTCCAGGAGCAGGCGGAGGCGCAGCGCCAGAAGGACCTGGCCGCCGCCGCCGCGAAGCTGGACG
  GCACCCTGAGCGTGGACCTGGCCAGCACCCAGAGGGCCGGCTCCAGTACCGTCCTGGTGCCCACCAGGCA
  CCCCCATGCCATCCCCGGGTCCATTTTGAGCCACAAGACTAGCAGAGACCGTGGCTCTCTTGGCCACGTC
  ACTTTTGGCGGCCTCAGCTCCAGCACTGGGCATTTGCCCAGCCACATCACGCACGGTGACCCCAACACTG
  GCCACGTGACCTTCGGCTCCACCAGTGCCTCGAGTGGGGGCCACGTGACCTTCAGACCCGTCAGCGCCAG
  CAGCTACCTGGGCTCCACTGGATACGTGGGGTCCAGCAGGGGCGGAGAAAACACAGAGGGTGGTGTGGAG
  AGCGGAGGCACAGCGTCTGATTCGAGCGGAGGCCTCGGGTCCAGCAGAGACCACGTCTCCAGCACCGGCC
  CTGCCTCCAGCACTGGCCCGGGCTCCTCCACCAGCAAAGACTCCCGGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229332 representing NM_144577
  Red=Cloning site Green=Tags(s)
  
  MEGERRAYSKEVHQRINKQLEEIRRLEEVRGDLQVQISAAQNQVKRLRDSQRLENMDRLLKGRAQVQAEI
  EELQEQTRALDKQIQEWETRIFTHSKNVRSPGFILDQKVKIRRRIRILENQLDRVTCHFDNQLVRNAALR
  EELDLLRIDRNRYLNVDRKLKKEIHHLHHLVSTLILSSTSAYAVREEAKAKMGLLRERAEKEEAQSEMEA
  QVLQRQILHLEQLHHFLKLKNNDRQPDPDVLEKREKQAGEVAEGVWKTSQERLVLCYEDALNKLSQLMGE
  SDPDLLVQKYLEIEERNFAEFNFINEQNLELEHVQEEIKEMQEALVSARASKDDQHLLQEQQQKVLQQRM
  DKVHSEAERLEARFQDVRGQLEKLKADIQLLFTKAHCDSSMIDDLLGVKTSMGDRDMGLFLSLIEKRLVE
  LLTVQAFLHAQSFTSLADAALLVLGQSLEDLPKKMAPLQPPDTLEDPPGFEASDDYPMSREELLSQVEKL
  VELQEQAEAQRQKDLAAAAAKLDGTLSVDLASTQRAGSSTVLVPTRHPHAIPGSILSHKTSRDRGSLGHV
  TFGGLSSSTGHLPSHITHGDPNTGHVTFGSTSASSGGHVTFRPVSASSYLGSTGYVGSSRGGENTEGGVE
  SGGTASDSSGGLGSSRDHVSSTGPASSTGPGSSTSKDSRG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144577
• ORF Size: 2010 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144577.4
• RefSeq ORF: 2013 bp
• Locus ID: 93233
• UniProt ID: Q96M63
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome
• MW: 74.9 kDa
• Gene Summary: This gene encodes a coiled-coil domain-containing protein that is a component of the outer dynein arm docking complex in cilia cells. Mutations in this gene may cause primary ciliary dyskinesia 20. [provided by RefSeq, May 2013]