GOLGA6L1 (NM_001001413) Human Tagged ORF Clone

CAT#: RC229330

GOLGA6L1 (Myc-DDK-tagged)-Human golgin A6 family-like 1 (GOLGA6L1). Note: ORF is codon optimized

Product Images

Circular map for RC229330

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GOLGA6L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229330 ORF sequence, codon optimized.
  Due to the complexity of NM_001001413, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGATGTGGCCTCAGCCACACCTGCCTACCCACCCTCATCTTCCTACACATCCTCATCTCCCGACAC
  ATCCTCATCTGCCCACCCACCCTCACCTTCCGACGCACCCTCACCTCCCCACTCACCCACACCTCCCAAC
  TCACCCAATGATGTCCAAGGAGACACGGCAGAGCAAGCTTGCGGAAGCTAAAGAACAGCTCACTGACCAC
  CATCCTCAGACCAATCCTAGCGTGGGTACCGCCGCGTCCGACACAAAAAAAAAGAAGATCAATAACGGCA
  CTAATCCCGAGACTACGACCAGTGGTGGGTGCCACTCCCCCGAGGATGAACAGAAGGCCTCCCATCAGCA
  TCAGGAAGCGTTGCGCCGGGAACTGGAGGCCCAGGTTCACACAATCCGGATTCTCACCTGCCAGAAGACA
  GAACTCCAGATGGCCTTGTATTACTCTCAGCACGCGGTGAAGCAGCTCGAGGGGGAGGCCCGCGACCTGA
  TTTCCAGGCTCCACGACAGTTGGAAGTTTGCCGGAGAGCTGGAGCAGGCCTTGTCTGCCGTCGCCACACA
  GAAGAAGAAAGCCGACCGCTACATAGAAGAACTGACCAAAGAGAGGGATGCACTGAGCCTGGAACTGTAT
  CGGAATACCATCACTGACGAAGAACTGAAAGAAAAGAATGCAAAACTTCAGGAGAAGTTGCAGCTGGTGG
  AGTCAGAAAAATCTGAAATTCAACTGAATGTGAAGGAACTCAAACGCAAACTCGAAAGGGCGAAACTGCT
  TTTGCCTCAGCAACAGCTCCAAGCGGAAGCCGATCATCTGGGAAAAGAACTGCAGAGCGTGAGCGCTAAG
  CTCCAGGCACAGGTTGAAGAAAATGAGCTCTGGAATCGGCTGAACCAGCAGCAGGAGGAAAAGATGTGGC
  GGCAGGAGGAGAAGATCCAGGAGTGGGAGGAAAAAATCCAGGAGCAGGAAGAGAAAATCCGCGAACAGGA
  GGAGAAGATCCGCGAGCAGGAGGAGAAGATGCGCCGCCAGGAGGAAATGATGTGGGAGAAGGAAGAGAAA
  ATGCGGAGACAGGAAGAAATGATGTGGGAAAAAGAAGAGAAAATGCGGAGACTCGAAGAGATGATGTGGG
  AGAAGGAGGAGAAGATTAGAGAGTTGGAAGAAAAAATGCATGAACAGGAGAAGATCCGGGAACAGGAGGA
  GAAGCGCCAGGAAGAAGAGAAAATCCGGGAACAGGAAAAACGGCAAGAACAAGAAGCCAAGATGTGGCGC
  CAGGAGGAAAAGATACGGGAGCAGGAGGAAAAGATCAGGGAACAGGAAAAAAAAATGTGGCGCCAGGAGG
  AGAAAATCCACGAGCAAGAAAAGATTAGGGAAGAAGAAAAACGCCAGGAGCAGGAGGAGATGTGGCGGCA
  GGAGGAGAAGATACGCGAGCAGGAAGAGATTTGGCGGCAAAAAGAGAAGATGCATGAACAGGAGAAAATT
  CGCAAACAGGAGGAAAAGGTGTGGCGGCAAGAGGAGAAGATGCATGACCAGGAAGAGAAGATCAGGGAAC
  AGGAAGAGAAAATGTGGAGACAGGAGGAAAAGATACGCGAACAGGAAGAGAAAATCCGGGAGCAGGAGGA
  AAAGATTCGCGAACAGGAGGAAATGATGCAGGAGCAGGAGGAGAAAATGGGGGAGCAGGAAGAAAAAATG
  CAGGAGCAAGAAAAAATGCGAAGGCAGGAAGAGAAAATCCGCGAACAGGAGGAAAAGATCCGGGAACAGA
  AAGAAAAGATCCGGGAGCAGGAAGAGAAAATATGGGAGCAGGAGGAAAAGATAAGAGAACAGGAAGAAAT
  GATGCAAGAGCAAGAAGAAAAAATGTGGGAACAAGAAGAAAAGATGTGTGAACAGGAGGAAAAGATGCAG
  GAGCAAGAAGAGAAAATGAGAAGGCAGGAGGAGAAAATGTGGGAGCAGGAAGTGAGACTCCGACAGCAGG
  AAGAGAAAATGCAGGAACACCAGGAACACCTGGAGGCCGCGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229330 representing NM_001001413
  Red=Cloning site Green=Tags(s)
  
  MLMWPQPHLPTHPHLPTHPHLPTHPHLPTHPHLPTHPHLPTHPHLPTHPMMSKETRQSKLAEAKEQLTDH
  HPQTNPSVGTAASDTKKKKINNGTNPETTTSGGCHSPEDEQKASHQHQEALRRELEAQVHTIRILTCQKT
  ELQMALYYSQHAVKQLEGEARDLISRLHDSWKFAGELEQALSAVATQKKKADRYIEELTKERDALSLELY
  RNTITDEELKEKNAKLQEKLQLVESEKSEIQLNVKELKRKLERAKLLLPQQQLQAEADHLGKELQSVSAK
  LQAQVEENELWNRLNQQQEEKMWRQEEKIQEWEEKIQEQEEKIREQEEKIREQEEKMRRQEEMMWEKEEK
  MRRQEEMMWEKEEKMRRLEEMMWEKEEKIRELEEKMHEQEKIREQEEKRQEEEKIREQEKRQEQEAKMWR
  QEEKIREQEEKIREQEKKMWRQEEKIHEQEKIREEEKRQEQEEMWRQEEKIREQEEIWRQKEKMHEQEKI
  RKQEEKVWRQEEKMHDQEEKIREQEEKMWRQEEKIREQEEKIREQEEKIREQEEMMQEQEEKMGEQEEKM
  QEQEKMRRQEEKIREQEEKIREQKEKIREQEEKIWEQEEKIREQEEMMQEQEEKMWEQEEKMCEQEEKMQ
  EQEEKMRRQEEKMWEQEVRLRQQEEKMQEHQEHLEAAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001413
• ORF Size: 2004 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001413.3, NP_001001413.3
• RefSeq Size: 3822 bp
• RefSeq ORF: 2007 bp
• Locus ID: 283767
• UniProt ID: Q8N7Z2
• Cytogenetics: 15q11.2
• MW: 83.1 kDa