DACT3 (NM_145056) Human Tagged ORF Clone

CAT#: RC229315

DACT3 (Myc-DDK-tagged)-Human dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3)

Product Images

Circular map for RC229315

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DACT3
• Synonyms: DAPPER3; RRR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229315 representing NM_145056.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATCCGGGCCTTCTCGTTCCCGGTGAGCCCTGAGCGGGGCCGGCTGCGGGGCTGGCTGGAGGGTAGC
  CTGGCCGGGCTCTGCGAGTTACATTGGCTCCGGGAGAGGCAGGAGTACCGCGTGCAGCAGGCGCTGCGG
  CTGGCCCAGCCCGGAATGGGGGGCGCCGAGGCCGAGGACGAGGAGGACGCCGATGAGGATGAAGATGCG
  GCGGCGGCGCGCCGGGCCGCAGCGGCCCTGGAGGAGCAGCTGGAGGCCCTGCCTGGTCTCGTCTGGGAC
  CTGGGACAGCAGCTGGGAGACCTGAGCCTGGAGTCTGGGGGCCTGGAACAGGAGAGCGGGCGTAGCTCG
  GGCTTCTATGAAGATCCCAGCTCTACAGGAGGTCCAGATTCACCACCCTCAACCTTCTGTGGGGACAGT
  GGCTTCTCTGGATCCAGCTCCTATGGTCGCCTGGGTCCCTCTGAGCCCCGGGGCATCTATGCCAGTGAG
  AGGCCCAAGTCCCTAGGAGACGCCAGTCCCAGCGCTCCGGAGGTGGTGGGCGCGCGGGCAGCGGTGCCG
  CGGTCCTTCTCAGCGCCCTACCCGACGGCAGGGGGGTCCGCCGGCCCGGAGGCCTGCTCCTCGGCGGAG
  CGGCGGGCCCGCGCCGGGCCCTTTCTGACGCCCAGCCCCCTGCACGCCGTGGCGATGCGCAGCCCGCGG
  CCCTGCGGCCGCCCTCCCACCGACTCGCCCGACGCGGGGGGCGCAGGGCGGCCCCTGGACGGCTACATC
  TCGGCGCTCCTGCGCAGGCGCCGCCGCCGGGGGGCGGGCCAGCCCCGGACCAGTCCCGGGGGCGCGGAC
  GGCGGCCCGCGGCGCCAGAACAGCGTGCGCCAGCGGCCGCCCGACGCGTCTCCGTCCCCCGGCAGCGCG
  CGACCCGCGCGGGAGCCCTCGTTGGAGCGCGTCGGGGGCCACCCCACCAGCCCTGCCGCCTTGAGCCGC
  GCCTGGGCGTCGTCGTGGGAGTCGGAGGCGGCACCCGAGCCCGCTGCGCCGCCCGCCGCCCCCTCACCC
  CCCGACAGCCCGGCTGAGGGCCGCTTGGTGAAGGCGCAGTACATCCCGGGCGCGCAGGCGGCCACCCGA
  GGCCTCCCTGGCCGCGCCGCCCGCCGCAAACCGCCGCCACTGACCCGCGGCCGCAGCGTGGAGCAGTCA
  CCACCCCGGGAGCGTCCCCGGGCCGCCGGCCGCCGTGGACGCATGGCCGAGGCTTCGGGCCGCCGCGGC
  TCGCCCAGGGCCCGCAAGGCCTCGCGCTCCCAGTCTGAGACCAGCCTGCTGGGCCGCGCCTCCGCGGTC
  CCTTCGGGGCCCCCTAAGTACCCCACGGCGGAGCGGGAAGAGCCTCGGCCTCCACGGCCACGCCGCGGC
  CCAGCGCCCACGCTGGCGGCCCAGGCCGCAGGGTCCTGCCGTCGCTGGCGCTCCACTGCGGAGATCGAC
  GCTGCCGATGGGCGCCGCGTGCGGCCCCGAGCCCCTGCGGCGCGTGTTCCCGGCCCCGGCCCGTCCCCG
  TCAGCTCCCCAGCGTCGTCTGCTTTACGGCTGCGCGGGCAGCGACTCCGAGTGCTCGGCTGGGCGCCTG
  GGGCCCCTGGGACGCCGGGGGCCTGCGGGAGGCGTCGGCGGGGGTTACGGGGAGAGCGAATCGAGCGCC
  AGCGAGGGAGAATCGCCTGCCTTCAGCTCTGCCTCCAGCGACTCAGACGGCAGCGGTGGCCTCGTGTGG
  CCGCAGCAGCTGGTGGCGGCCACCGCGGCCTCTGGGGGTGGAGCAGGTGCAGGGGCGCCCGCAGGCCCC
  GCCAAAGTCTTCGTGAAAATCAAAGCTTCCCACGCGCTCAAGAAAAAGATACTGCGTTTCCGTTCGGGT
  TCTCTCAAGGTCATGACTACAGTG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC229315
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MIRAFSFPVSPERGRLRGWLEGSLAGLCELHWLRERQEYRVQQALRLAQPGMGGAEAEDEEDADEDEDA
  AAARRAAAALEEQLEALPGLVWDLGQQLGDLSLESGGLEQESGRSSGFYEDPSSTGGPDSPPSTFCGDS
  GFSGSSSYGRLGPSEPRGIYASERPKSLGDASPSAPEVVGARAAVPRSFSAPYPTAGGSAGPEACSSAE
  RRARAGPFLTPSPLHAVAMRSPRPCGRPPTDSPDAGGAGRPLDGYISALLRRRRRRGAGQPRTSPGGAD
  GGPRRQNSVRQRPPDASPSPGSARPAREPSLERVGGHPTSPAALSRAWASSWESEAAPEPAAPPAAPSP
  PDSPAEGRLVKAQYIPGAQAATRGLPGRAARRKPPPLTRGRSVEQSPPRERPRAAGRRGRMAEASGRRG
  SPRARKASRSQSETSLLGRASAVPSGPPKYPTAEREEPRPPRPRRGPAPTLAAQAAGSCRRWRSTAEID
  AADGRRVRPRAPAARVPGPGPSPSAPQRRLLYGCAGSDSECSAGRLGPLGRRGPAGGVGGGYGESESSA
  SEGESPAFSSASSDSDGSGGLVWPQQLVAATAASGGGAGAGAPAGPAKVFVKIKASHALKKKILRFRSG
  SLKVMTTV
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145056
• ORF Size: 1887 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145056.3
• RefSeq ORF: 1890 bp
• Locus ID: 147906
• UniProt ID: Q96B18
• Cytogenetics: 19q13.32
• MW: 64.9 kDa
• Gene Summary: May be involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins.[UniProtKB/Swiss-Prot Function]