CEECAM1 (CERCAM) (NM_016174) Human Tagged ORF Clone

CAT#: RC229302

CERCAM (Myc-DDK-tagged)-Human cerebral endothelial cell adhesion molecule (CERCAM)

Product Images

Circular map for RC229302

• 

  Western blot validation of overexpression lysate (Cat# LY432319) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229302 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CEECAM1
• Synonyms: CEECAM1; GLT25D3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229302 representing NM_016174
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCTGCCCGCGCCGCGCCGCTGCTCCAGCTGCTGCTCCTGCTGGGGCCGTGGCTGGAGGCTGCGG
  GCGTTGCGGAGTCGCCGCTGCCCGCCGTGGTCCTTGCCATCCTGGCCCGCAATGCCGAACACTCGCTGCC
  CCACTACCTGGGCGCTCTGGAGCGGCTGGACTACCCCCGGGCCAGGATGGCCCTCTGGTGTGCCACGGAC
  CACAATGTGGACAACACCACAGAGATGCTGCAGGAGTGGCTGGCGGCTGTGGGCGATGACTATGCTGCTG
  TGGTCTGGAGGCCTGAGGGCGAGCCCAGGTTCTACCCAGATGAAGAGGGTCCCAAGCACTGGACCAAAGA
  AAGGCACCAGTTTCTGATGGAGCTGAAGCAGGAAGCCCTCACCTTTGCCAGGAACTGGGGGGCCGACTAT
  ATCCTGTTTGCAGACACAGACAACATTCTGACCAACAATCAGACTCTGCGGCTTCTCATGGGGCAGGGGC
  TTCCAGTGGTGGCCCCAATGCTGGACTCCCAGACCTACTACTCCAACTTCTGGTGTGGGATCACCCCCCA
  GGGCTACTACCGCCGCACAGCCGAGTACTTCCCCACCAAGAACCGCCAGCGCCGGGGCTGCTTCCGTGTC
  CCCATGGTCCACTCCACCTTCCTTGCATCCCTGCGGGCTGAAGGGGCAGACCAGCTTGCTTTCTACCCGC
  CACATCCCAACTACACTTGGCCTTTCGACGACATCATCGTCTTCGCCTATGCCTGCCAGGCTGCTGGGGT
  CTCCGTCCACGTGTGCAATGAGCACCGTTATGGGTACATGAATGTGCCGGTGAAATCCCACCAGGGGCTG
  GAAGACGAGAGGGTCAACTTCATCCACCTGATCTTAGAAGCACTAGTGGACGGCCCCCGCATGCAGGCCT
  CAGCTCATGTGACTCGGCCCTCTAAGAGGCCCAGCAAGATAGGGTTTGACGAGGTCTTTGTCATCAGCCT
  GGCTCGCAGGCCTGACCGTCGGGAACGCATGCTCGCCTCGCTCTGGGAGATGGAGATCTCTGGGAGGGTG
  GTGGACGCTGTGGATGGCTGGATGCTCAACAGCAGTGCCATCAGGAACCTCGGCGTAGACCTGCTCCCGG
  GCTACCAGGACCCTTACTCGGGCCGCACTCTGACCAAGGGCGAGGTGGGCTGCTTCCTCAGCCATTACTC
  CATCTGGGAAGAGGTGGTTGCCAGGGGCCTGGCCCGGGTCCTGGTGTTTGAGGATGACGTGCGCTTTGAG
  AGCAACTTCAGGGGGCGGCTGGAGCGGCTGATGGAGGATGTGGAGGCAGAGAAACTGTCTTGGGACCTGA
  TCTACCTCGGACGGAAGCAGGTGAACCCTGAGAAGGAGACGGCCGTGGAGGGGCTGCCGGGCCTGGTGGT
  GGCTGGGTACTCCTACTGGACGCTGGCCTATGCCCTGCGTCTGGCGGGTGCCCGCAAGCTGCTGGCCTCA
  CAGCCTCTGCGCCGCATGCTGCCCGTGGACGAGTTCCTGCCCATCATGTTCGACCAGCACCCCAACGAGC
  AGTACAAGGCACACTTCTGGCCACGGGACCTGGTGGCCTTCTCCGCCCAGCCCCTGCTCGCTGCCCCTAC
  CCACTATGCCGGGGACGCCGAGTGGCTCAGTGACACGGAGACATCCTCTCCATGGGATGATGACAGCGGC
  CGCCTCATCAGCTGGAGCGGCTCCCAAAAGACCCTGCGCAGCCCCCGCCTGGACCTGACTGGCAGCAGCG
  GGCACAGCCTCCAACCCCAGCCCCGAGATGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229302 representing NM_016174
  Red=Cloning site Green=Tags(s)
  
  MRAARAAPLLQLLLLLGPWLEAAGVAESPLPAVVLAILARNAEHSLPHYLGALERLDYPRARMALWCATD
  HNVDNTTEMLQEWLAAVGDDYAAVVWRPEGEPRFYPDEEGPKHWTKERHQFLMELKQEALTFARNWGADY
  ILFADTDNILTNNQTLRLLMGQGLPVVAPMLDSQTYYSNFWCGITPQGYYRRTAEYFPTKNRQRRGCFRV
  PMVHSTFLASLRAEGADQLAFYPPHPNYTWPFDDIIVFAYACQAAGVSVHVCNEHRYGYMNVPVKSHQGL
  EDERVNFIHLILEALVDGPRMQASAHVTRPSKRPSKIGFDEVFVISLARRPDRRERMLASLWEMEISGRV
  VDAVDGWMLNSSAIRNLGVDLLPGYQDPYSGRTLTKGEVGCFLSHYSIWEEVVARGLARVLVFEDDVRFE
  SNFRGRLERLMEDVEAEKLSWDLIYLGRKQVNPEKETAVEGLPGLVVAGYSYWTLAYALRLAGARKLLAS
  QPLRRMLPVDEFLPIMFDQHPNEQYKAHFWPRDLVAFSAQPLLAAPTHYAGDAEWLSDTETSSPWDDDSG
  RLISWSGSQKTLRSPRLDLTGSSGHSLQPQPRDEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016174
• ORF Size: 1785 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016174.5
• RefSeq ORF: 1788 bp
• Locus ID: 51148
• UniProt ID: Q5T4B2
• Cytogenetics: 9q34.11
• Domains: Glyco_transf_25
• MW: 67.4 kDa
• Gene Summary: Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Does not express any beta-galactosyltransferase activity in vitro.[UniProtKB/Swiss-Prot Function]