THUMPD2 (NM_025264) Human Tagged ORF Clone

CAT#: RC229254

THUMPD2 (Myc-DDK-tagged)-Human THUMP domain containing 2 (THUMPD2), transcript variant 1

Product Images

Circular map for RC229254

• 

  Western blot validation of overexpression lysate (Cat# LY432274) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229254 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: THUMPD2
• Synonyms: C2orf8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229254 representing NM_025264
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAGGCGCGTGGAGAGCCAGGGTCCGGGCCTGAGGCTGGCGCCCGATTCTTCTGCACTGCGGGTC
  GCGGCCTGGAGCCGTTCGTAATGCGAGAGGTGCGGGCGCGGCTGGCGGCCACGCAGGTTGAATATATTTC
  AGGAAAGGTTTTTTTCACCACCTGTTCTGATTTGAATATGTTGAAGAAATTAAAATCTGCAGAAAGATTA
  TTTTTGCTGATTAAAAAGCAGTTTCCACTTATTATTTCTTCTGTAAGTAAAGGAAAAATATTTAATGAAA
  TGCAAAGACTTATAAATGAAGATCCAGGAAGTTGGTTGAATGCCATTTCAATTTGGAAAAATCTTCTTGA
  ACTTGATGCAAAAAAGGAAAAACTTTCTCAGAGAGATGATAACCAACTAAAAAGAAAAGTGGGAGAAAAT
  GAAATCATTGCAAAGAAATTAAAAATAGAACAAATGCAAAAGATAGAAGAGAATAGGGACTGCCAGCTGG
  AAAAACAAATAAAAGAAGAAACTCTGGAGCAAAGAGATTTTACCACTAAAAGCGAAAAGTTTCAAGAAGA
  AGAATTTCAGAATGACATAGAGAAAGCAATTGATACTCATAATCAGAATGACTTGACTTTCAGAGTATCT
  TGTCGCTGCAGTGGAACTATTGGAAAGGCCTTCACTGCACAGGAGGTAGGAAAAGTAATTGGAATTGCTA
  TTATGAAACACTTTGGATGGAAAGCAGACTTGAGGAATCCACAATTAGAGATCTTTATACATCTAAATGA
  CATTTACTCTGTGGTGGGGATTCCTGTGTTCAGGGTTTCCCTAGCCAGCAGAGCTTACATCAAGACAGCT
  GGACTGCGATCTACAATAGCGTGGGCAATGGCATCTCTGGCTGACATTAAGGCTGGTGCATTTGTTTTAG
  ATCCAATGTGTGGACTTGGAACAATACTTTTGGAAGCTGCTAAAGAATGGCCAGATGTGTATTATGTAGG
  TGCTGATGTCAGCGACTCACAGTTACTAGGTACTTGGGACAATCTGAAAGCTGCAGGCCTTGAGGATAAA
  ATTGAATTACTTAAAATCTCTGTTATAGAATTGCCATTGCCTTCAGAAAGTGTTGATATTATTATTTCTG
  ACATTCCATTTGGGAAAAAGTTTAAGTTAGGAAAAGACATCAAAAGCATTCTACAAGAAATGGAAAGAGT
  GCTTCATGTTGGCGGAACCATTGTATTGTTGCTTAGTGAAGATCACCACAGGCGCCTTACAGATTGTAAA
  GAGAGCAACATCCCTTTCAATTCCAAGGACAGTCACACAGATGAACCTGGAATTAAAAAGTGCTTGAATC
  CTGAAGAAAAAACTGGTGCATTCAAGACAGCGTCAACTTCATTCGAAGCCAGTAACCACAAATTCTTAGA
  CAGAATGTCACCATTTGGCTCCTTGGTACCAGTGGAATGCTACAAAGTTAGCCTTGGAAAGACAGATGCG
  TTCATATGTAAATATAAGAAGTCGCACTCTTCTGGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229254 representing NM_025264
  Red=Cloning site Green=Tags(s)
  
  MSEARGEPGSGPEAGARFFCTAGRGLEPFVMREVRARLAATQVEYISGKVFFTTCSDLNMLKKLKSAERL
  FLLIKKQFPLIISSVSKGKIFNEMQRLINEDPGSWLNAISIWKNLLELDAKKEKLSQRDDNQLKRKVGEN
  EIIAKKLKIEQMQKIEENRDCQLEKQIKEETLEQRDFTTKSEKFQEEEFQNDIEKAIDTHNQNDLTFRVS
  CRCSGTIGKAFTAQEVGKVIGIAIMKHFGWKADLRNPQLEIFIHLNDIYSVVGIPVFRVSLASRAYIKTA
  GLRSTIAWAMASLADIKAGAFVLDPMCGLGTILLEAAKEWPDVYYVGADVSDSQLLGTWDNLKAAGLEDK
  IELLKISVIELPLPSESVDIIISDIPFGKKFKLGKDIKSILQEMERVLHVGGTIVLLLSEDHHRRLTDCK
  ESNIPFNSKDSHTDEPGIKKCLNPEEKTGAFKTASTSFEASNHKFLDRMSPFGSLVPVECYKVSLGKTDA
  FICKYKKSHSSGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025264
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025264.5
• RefSeq ORF: 1512 bp
• Locus ID: 80745
• UniProt ID: Q9BTF0
• Cytogenetics: 2p22-p21
• Protein Families: Druggable Genome
• MW: 56.3 kDa