PCBP3 (NM_020528) Human Tagged ORF Clone

CAT#: RC229176

PCBP3 (Myc-DDK-tagged)-Human poly(rC) binding protein 3 (PCBP3), transcript variant 1

Product Images

Circular map for RC229176

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  Western blot validation of overexpression lysate (Cat# LY432200) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229176 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PCBP3 protein (Cat# TP329176). The protein was produced from HEK293T cells transfected with PCBP3 cDNA clone (Cat# RC229176) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCBP3
• Synonyms: ALPHA-CP3; PCBP3-OT1; PCBP3OT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229176 representing NM_020528
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAAGGTGACGCCTTCTGGGCCCCATCTGTCCTTCCTCACAGCACCCTCAGCACCTTAAGCCACC
  ACCCTCAGCCACAATTTGGCAGAAGGATGGAGTCCAAGGTCTCAGAAGGTGGCCTGAATGTGACCCTCAC
  CATCCGCCTGCTGATGCATGGAAAGGAAGTTGGAAGCATCATCGGGAAGAAAGGAGAAACTGTGAAGAAG
  ATGCGTGAGGAGAGTGGTGCAAGGATCAACATCTCAGAGGGAAACTGCCCAGAGAGGATTGTGACCATCA
  CAGGCCCCACAGACGCCATCTTCAAGGCCTTTGCCATGATCGCATACAAGTTTGAGGAGGATATCATCAA
  CTCCATGAGCAACAGCCCTGCCACCAGCAAGCCCCCAGTGACGCTGAGGCTGGTGGTGCCTGCCAGCCAG
  TGTGGGTCCCTGATCGGCAAAGGAGGCTCCAAGATCAAGGAGATCAGGGAGTCCACAGGTGCCCAGGTGC
  AGGTGGCTGGGGACATGCTGCCCAACTCCACGGAGCGAGCGGTGACCATCTCGGGGACCCCAGATGCCAT
  CATCCAGTGCGTCAAGCAGATCTGTGTGGTCATGCTGGAGTCCCCACCGAAAGGTGCCACCATTCCCTAC
  CGCCCAAAGCCCGCCTCCACCCCTGTCATTTTTGCAGGTGGTCAGGCCTACACAATCCAGGGACAGTATG
  CCATCCCTCACCCGGATCAGTTGACCAAGCTCCACCAGTTGGCCATGCAGCAAACCCCCTTTCCTCCCCT
  CGGACAGACCAACCCCGCTTTCCCCGGAGAAAAGCTGCCTTTACACTCCTCCGAAGAAGCTCAAAATCTG
  ATGGGCCAGTCATCAGGTCTGGACGCCAGCCCACCGGCCAGCACTCATGAGCTCACCATTCCCAATGATC
  TAATAGGCTGCATAATTGGACGCCAAGGGACCAAAATCAATGAAATTCGACAGATGTCTGGAGCTCAGAT
  CAAAATCGCCAACGCCACGGAAGGGTCCTCAGAGCGTCAGATCACCATCACGGGGACCCCGGCCAACATC
  AGCCTTGCCCAGTATCTCATCAACGCCAGGCTGACGTCCGAGGTCACCGGGATGGGCACGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229176 representing NM_020528
  Red=Cloning site Green=Tags(s)
  
  MGEGDAFWAPSVLPHSTLSTLSHHPQPQFGRRMESKVSEGGLNVTLTIRLLMHGKEVGSIIGKKGETVKK
  MREESGARINISEGNCPERIVTITGPTDAIFKAFAMIAYKFEEDIINSMSNSPATSKPPVTLRLVVPASQ
  CGSLIGKGGSKIKEIRESTGAQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLESPPKGATIPY
  RPKPASTPVIFAGGQAYTIQGQYAIPHPDQLTKLHQLAMQQTPFPPLGQTNPAFPGEKLPLHSSEEAQNL
  MGQSSGLDASPPASTHELTIPNDLIGCIIGRQGTKINEIRQMSGAQIKIANATEGSSERQITITGTPANI
  SLAQYLINARLTSEVTGMGTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020528
• ORF Size: 1113 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020528.3
• RefSeq ORF: 1116 bp
• Locus ID: 54039
• UniProt ID: P57721
• Cytogenetics: 21q22.3
• Domains: KH
• MW: 39.3 kDa
• Gene Summary: This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. The protein encoded by this gene lacks the nuclear localization signals found in other subfamily members. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2017]