HIV TAT specific factor 1 (HTATSF1) (NM_001163280) Human Tagged ORF Clone

CAT#: RC229011

HTATSF1 (Myc-DDK-tagged)-Human HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 1. Note: ORF is codon optimized

Product Images

Circular map for RC229011

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HIV TAT specific factor 1
• Synonyms: dJ196E23.2; TAT-SF1; TATSF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229011 ORF sequence, codon optimized.
  Due to the complexity of NM_001163280, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGACAAACCTGGACGGGAACGACGAGTTTGACGAGCAACTGCGAATGCAGGAGCTGTATGGCG
  ATGGGAAAGACGGTGACACACAGACCGATGCAGGCGGCGAGCCAGATTCTCTGGGCCAACAGCCAACAGA
  CACCCCATACGAATGGGACCTCGACAAGAAGGCCTGGTTCCCAAAGATTACAGAGGACTTTATCGCCACA
  TATCAGGCCAATTATGGTTTCAGCAATGACGGGGCTTCCTCAAGTACAGCTAACGTAGAGGATGTCCATG
  CCCGAACCGCGGAAGAACCTCCACAGGAAAAGGCCCCTGAGCCGACCGACGCGAGAAAGAAAGGGGAAAA
  GAGAAAGGCAGAGTCAGGCTGGTTCCATGTCGAGGAGGACCGCAATACCAACGTCTACGTGTCAGGATTG
  CCGCCCGACATAACCGTAGACGAGTTCATTCAGTTGATGTCTAAATTTGGCATTATCATGCGCGATCCCC
  AGACAGAAGAGTTCAAGGTGAAGCTGTACAAGGACAATCAGGGAAATCTGAAGGGCGATGGCCTGTGTTG
  TTACCTGAAGAGGGAGTCCGTCGAGTTGGCCTTGAAGCTCCTCGACGAAGATGAGATCAGAGGATATAAG
  CTTCATGTGGAGGTGGCCAAGTTCCAACTCAAAGGAGAATATGATGCAAGCAAAAAGAAGAAAAAGTGCA
  AGGATTACAAGAAAAAGCTGTCTATGCAGCAGAAACAGCTCGATTGGCGACCGGAGCGGAGAGCCGGCCC
  ATCCCGGATGAGGCATGAACGGGTGGTGATCATAAAGAACATGTTCCATCCAATGGATTTCGAGGATGAC
  CCACTTGTGTTGAACGAGATCCGGGAAGACCTTAGGGTTGAGTGCTCTAAGTTCGGCCAGATTCGAAAGC
  TGCTGCTGTTCGATCGCCACCCAGACGGTGTCGCCTCAGTCAGTTTTAGAGATCCAGAAGAGGCGGACTA
  TTGCATCCAGACTCTCGACGGGCGGTGGTTTGGAGGGCGCCAGATTACAGCGCAGGCTTGGGACGGCACA
  ACAGACTATCAAGTCGAAGAGACATCCAGAGAGCGGGAAGAACGCCTGCGAGGCTGGGAGGCTTTTCTGA
  ACGCACCTGAAGCCAATCGGGGACTTAGACGGAGTGATTCAGTGAGCGCGTCCGAACGCGCTGGCCCTTC
  CCGGGCCAGGCATTTCAGCGAGCACCCTAGCACATCCAAGATGAATGCGCAGGAAACCGCTACCGGCATG
  GCCTTTGAAGAACCTATTGATGAAAAGAAGTTTGAGAAAACTGAAGACGGAGGCGAATTCGAGGAGGGGG
  CCTCAGAAAATAATGCCAAGGAATCAAGCCCAGAGAAAGAGGCGGAGGAGGGTTGCCCAGAAAAGGAATC
  TGAAGAAGGATGTCCTAAAAGGGGGTTTGAGGGCTCTTGCAGTCAGAAGGAAAGCGAGGAAGGCAACCCG
  GTGCGCGGCTCAGAGGAAGACAGCCCTAAGAAGGAAAGCAAGAAAAAGACCCTCAAAAACGATTGTGAGG
  AAAACGGACTGGCCAAAGAATCTGAAGACGATCTGAACAAGGAAAGTGAAGAAGAGGTTGGACCAACGAA
  AGAGTCTGAAGAAGATGACTCCGAGAAGGAAAGTGATGAGGACTGTTCTGAAAAACAATCCGAGGACGGG
  TCAGAGAGGGAATTCGAGGAGAATGGACTCGAGAAAGATCTGGACGAGGAGGGATCCGAAAAGGAGCTGC
  ACGAGAACGTGCTGGACAAAGAGCTCGAGGAGAACGATTCCGAAAACTCCGAATTCGAGGACGACGGCAG
  TGAAAAGGTATTGGATGAGGAGGGCTCTGAGAGGGAGTTTGACGAAGACTCCGATGAGAAAGAAGAGGAG
  GAAGATACCTATGAGAAAGTGTTCGACGACGAAAGTGATGAGAAGGAGGATGAGGAATATGCGGACGAAA
  AGGGTCTGGAGGCCGCTGATAAAAAGGCTGAGGAGGGGGATGCGGATGAGAAGTTGTTTGAAGAGTCTGA
  CGACAAAGAAGATGAAGATGCTGATGGGAAAGAGGTAGAGGATGCTGATGAAAAGCTCTTCGAAGATGAC
  GATTCAAACGAGAAACTGTTTGATGAAGAGGAAGACAGTAGCGAAAAACTGTTCGACGATAGCGATGAGA
  GAGGCACATTGGGCGGATTTGGGTCAGTAGAAGAGGGCCCCCTCTCTACTGGGTCTAGCTTCATTCTGAG
  CTCTGATGATGATGACGACGATATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229011 representing NM_001163280
  Red=Cloning site Green=Tags(s)
  
  MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIAT
  YQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGL
  PPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYK
  LHVEVAKFQLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKNMFHPMDFEDD
  PLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFRDPEEADYCIQTLDGRWFGGRQITAQAWDGT
  TDYQVEETSREREERLRGWEAFLNAPEANRGLRRSDSVSASERAGPSRARHFSEHPSTSKMNAQETATGM
  AFEEPIDEKKFEKTEDGGEFEEGASENNAKESSPEKEAEEGCPEKESEEGCPKRGFEGSCSQKESEEGNP
  VRGSEEDSPKKESKKKTLKNDCEENGLAKESEDDLNKESEEEVGPTKESEEDDSEKESDEDCSEKQSEDG
  SEREFEENGLEKDLDEEGSEKELHENVLDKELEENDSENSEFEDDGSEKVLDEEGSEREFDEDSDEKEEE
  EDTYEKVFDDESDEKEDEEYADEKGLEAADKKAEEGDADEKLFEESDDKEDEDADGKEVEDADEKLFEDD
  DSNEKLFDEEEDSSEKLFDDSDERGTLGGFGSVEEGPLSTGSSFILSSDDDDDDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163280
• ORF Size: 2265 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163280.1, NP_001156752.1
• RefSeq Size: 3037 bp
• RefSeq ORF: 2268 bp
• Locus ID: 27336
• UniProt ID: O43719
• Cytogenetics: Xq26.3
• Protein Families: Transcription Factors
• MW: 85.9 kDa
• Gene Summary: The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Sep 2009]